NSP_SLCV
ID NSP_SLCV Reviewed; 256 AA.
AC P21935;
DT 01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT 18-MAR-2008, sequence version 2.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=Nuclear shuttle protein;
DE Short=NSP;
DE AltName: Full=Protein BR1;
DE AltName: Full=Protein BV1;
GN ORFNames=BR1, BV1;
OS Squash leaf curl virus (SLCV).
OC Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes;
OC Geplafuvirales; Geminiviridae; Begomovirus.
OX NCBI_TaxID=10829;
OH NCBI_TaxID=3662; Cucurbita moschata (Winter crookneck squash) (Cucurbita pepo var. moschata).
OH NCBI_TaxID=3663; Cucurbita pepo (Vegetable marrow) (Summer squash).
OH NCBI_TaxID=3885; Phaseolus vulgaris (Kidney bean) (French bean).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1984668; DOI=10.1016/0042-6822(91)90009-z;
RA Lazarowitz S.G., Lazdins I.B.;
RT "Infectivity and complete nucleotide sequence of the cloned genomic
RT components of a bipartite squash leaf curl geminivirus with a broad host
RT range phenotype.";
RL Virology 180:58-69(1991).
RN [2]
RP SUBCELLULAR LOCATION.
RX PubMed=12242403; DOI=10.2307/3870094;
RA Sanderfoot A.A., Lazarowitz S.G.;
RT "Cooperation in viral movement: the geminivirus BL1 movement protein
RT interacts with BR1 and redirects it from the nucleus to the eell
RT periphery.";
RL Plant Cell 7:1185-1194(1995).
RN [3]
RP FUNCTION, NUCLEAR LOCALIZATION SIGNAL, AND MUTAGENESIS OF 25-LYS-ARG-26;
RP 36-ARG--ARG-38; 89-ARG--ARG-91; 97-LYS--ARG-100; 148-PHE-ASP-149;
RP 170-ASP-ARG-171; 201-ASN--ARG-203; ASP-212; 215-ARG-ASP-216; ASN-224 AND
RP 227-LYS-ASN-228.
RX PubMed=8587985; DOI=10.1104/pp.110.1.23;
RA Sanderfoot A.A., Ingham D.J., Lazarowitz S.G.;
RT "A viral movement protein as a nuclear shuttle. The geminivirus BR1
RT movement protein contains domains essential for interaction with BL1 and
RT nuclear localization.";
RL Plant Physiol. 110:23-33(1996).
RN [4]
RP NUCLEAR EXPORT SIGNAL, AND MUTAGENESIS OF 189-LEU--LEU-193.
RX PubMed=10402428; DOI=10.2307/3870748;
RA Ward B.M., Lazarowitz S.G.;
RT "Nuclear export in plants. Use of geminivirus movement proteins for a cell-
RT based export assay.";
RL Plant Cell 11:1267-1276(1999).
CC -!- FUNCTION: Binds to the genomic viral ssDNA, shuttles it into and out of
CC the cell nucleus. Begomoviruses use 2 proteins to transport their DNA
CC from cell to cell. The nuclear shuttle protein (NSP) shuttles it
CC between nucleus and cytoplasm and the movement protein (MP) probably
CC transports the DNA-NSP complex to the cell periphery and facilitates
CC movement across the cell wall. {ECO:0000269|PubMed:8587985}.
CC -!- SUBUNIT: Binds to single-stranded and double-stranded viral DNA.
CC Interacts with the host nuclear shuttle interacting (NSI) protein. This
CC interaction may allow NSP to recruit NSI monomers to the viral genome
CC and thus regulate nuclear export of viral genome by NSP (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000269|PubMed:12242403}. Host
CC cytoplasm {ECO:0000269|PubMed:12242403}. Host cell membrane
CC {ECO:0000269|PubMed:12242403}; Peripheral membrane protein
CC {ECO:0000269|PubMed:12242403}; Cytoplasmic side
CC {ECO:0000269|PubMed:12242403}. Note=Translocated to the plasma membrane
CC by the movement protein BC1.
CC -!- SIMILARITY: Belongs to the begomovirus nuclear shuttle protein family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC32408.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; M38182; AAC32408.1; ALT_INIT; Genomic_DNA.
DR PIR; A36785; QQCVSV.
DR RefSeq; NP_047247.2; NC_001937.1.
DR GeneID; 956398; -.
DR KEGG; vg:956398; -.
DR Proteomes; UP000009151; Genome.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0019028; C:viral capsid; IEA:InterPro.
DR GO; GO:0003697; F:single-stranded DNA binding; IEA:InterPro.
DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR GO; GO:0051027; P:DNA transport; IEA:InterPro.
DR GO; GO:0046740; P:transport of virus in host, cell to cell; IEA:UniProtKB-KW.
DR InterPro; IPR001530; Gemini_BR1.
DR InterPro; IPR000263; GV_A/BR1_coat.
DR Pfam; PF00844; Gemini_coat; 1.
DR PRINTS; PR00223; GEMCOATARBR1.
DR PRINTS; PR00225; GEMCOATBR1.
PE 1: Evidence at protein level;
KW DNA-binding; Host cell membrane; Host cytoplasm; Host membrane;
KW Host nucleus; Host-virus interaction; Membrane; Reference proteome;
KW Transport; Viral movement protein.
FT CHAIN 1..256
FT /note="Nuclear shuttle protein"
FT /id="PRO_0000222267"
FT REGION 1..46
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 150..187
FT /note="Interaction with Arabidopsis thaliana NSI protein"
FT /evidence="ECO:0000250"
FT MOTIF 21..42
FT /note="Bipartite nuclear localization signal"
FT MOTIF 81..96
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000269|PubMed:8587985"
FT MOTIF 177..198
FT /note="Nuclear export signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 27..43
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 25..26
FT /note="KR->AA: Delayed nuclear targeting. No effect on
FT infectivity."
FT /evidence="ECO:0000269|PubMed:8587985"
FT MUTAGEN 36..38
FT /note="RRR->AAA: No effect on subcellular location."
FT /evidence="ECO:0000269|PubMed:8587985"
FT MUTAGEN 89..91
FT /note="RTR->ATA: Mislocalizes to the cytoplasm. Complete
FT loss of infectivity."
FT /evidence="ECO:0000269|PubMed:8587985"
FT MUTAGEN 97..100
FT /note="KRVR->AAVA: No effect on subcellular location."
FT /evidence="ECO:0000269|PubMed:8587985"
FT MUTAGEN 148..149
FT /note="FD->AA: No effect on subcellular location."
FT /evidence="ECO:0000269|PubMed:8587985"
FT MUTAGEN 170..171
FT /note="DR->AA: No effect on subcellular location."
FT /evidence="ECO:0000269|PubMed:8587985"
FT MUTAGEN 189..193
FT /note="LLIDL->AAIDA: Complete loss of infectivity."
FT /evidence="ECO:0000269|PubMed:10402428"
FT MUTAGEN 201..203
FT /note="NKR->AAA: Delayed nuclear targeting. No effect on
FT infectivity."
FT /evidence="ECO:0000269|PubMed:8587985"
FT MUTAGEN 212
FT /note="D->A: Delayed nuclear targeting."
FT /evidence="ECO:0000269|PubMed:8587985"
FT MUTAGEN 215..216
FT /note="RD->AA: Delayed nuclear targeting."
FT /evidence="ECO:0000269|PubMed:8587985"
FT MUTAGEN 224
FT /note="N->A: Mislocalizes to the cytoplasm. Complete loss
FT of infectivity."
FT /evidence="ECO:0000269|PubMed:8587985"
FT MUTAGEN 227..228
FT /note="KN->AA: Mislocalizes to the cytoplasm. Complete loss
FT of infectivity."
FT /evidence="ECO:0000269|PubMed:8587985"
SQ SEQUENCE 256 AA; 29228 MW; 0EC879D1983DEFAF CRC64;
MYSTSNRRGR SQTQRGSHVR RTGVKRSYGA ARGDDRRRPN VVSKTQVEPR MTIQRVQENQ
FGPEFVLSQN SALSTFVTYP SYVKTVPNRT RTYIKLKRVR FKGTLKIERG QGDTIMDGPS
SNIEGVFSMV IVVDRKPHVS QSGRLHTFDE LFGARIHCHG NLSVVPALKD RYYIRHVTKR
VVSLEKDTLL IDLHGTTQLS NKRYNCWASF SDLERDSCNG VYGNITKNAL LVYYCWLSDA
QSKASTYVSF ELDYLG
