ID   NSP_TGMVY               Reviewed;         256 AA.
AC   P03564;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   23-FEB-2022, entry version 81.
DE   RecName: Full=Nuclear shuttle protein;
DE            Short=NSP;
DE   AltName: Full=Protein BR1;
DE   AltName: Full=Protein BV1;
GN   ORFNames=BR1, BV1;
OS   Tomato golden mosaic virus (strain Yellow vein) (TGMV).
OC   Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes;
OC   Geplafuvirales; Geminiviridae; Begomovirus.
OX   NCBI_TaxID=223341;
OH   NCBI_TaxID=4081; Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=16453557; DOI=10.1002/j.1460-2075.1984.tb02114.x;
RA   Hamilton W.D.O., Stein V.E., Coutts R.H.A., Buck K.W.;
RT   "Complete nucleotide sequence of the infectious cloned DNA components of
RT   tomato golden mosaic virus: potential coding regions and regulatory
RT   sequences.";
RL   EMBO J. 3:2197-2205(1984).
CC   -!- FUNCTION: Binds to the genomic viral ssDNA, shuttles it into and out of
CC       the cell nucleus. Begomoviruses use 2 proteins to transport their DNA
CC       from cell to cell. The nuclear shuttle protein (NSP) shuttles it
CC       between nucleus and cytoplasm and the movement protein (MP) probably
CC       transports the DNA-NSP complex to the cell periphery and facilitates
CC       movement across the cell wall (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Binds to single-stranded and double-stranded viral DNA.
CC       Interacts with the host nuclear shuttle interacting (NSI) protein. This
CC       interaction may allow NSP to recruit NSI monomers to the viral genome
CC       and thus regulate nuclear export of viral genome by NSP (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000250}. Host cytoplasm
CC       {ECO:0000250}. Host cell membrane {ECO:0000250}; Peripheral membrane
CC       protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}.
CC       Note=Translocated to the plasma membrane by the movement protein BC1.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the begomovirus nuclear shuttle protein family.
CC       {ECO:0000305}.
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DR   EMBL; K02030; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; A04167; QQCVRG.
DR   Proteomes; UP000007405; Genome.
DR   GO; GO:0044164; C:host cell cytosol; IDA:CAFA.
DR   GO; GO:0042025; C:host cell nucleus; IDA:CAFA.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019028; C:viral capsid; IEA:InterPro.
DR   GO; GO:0003697; F:single-stranded DNA binding; IEA:InterPro.
DR   GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR   GO; GO:0051027; P:DNA transport; IEA:InterPro.
DR   GO; GO:0046740; P:transport of virus in host, cell to cell; IEA:UniProtKB-KW.
DR   InterPro; IPR001530; Gemini_BR1.
DR   InterPro; IPR000263; GV_A/BR1_coat.
DR   Pfam; PF00844; Gemini_coat; 1.
DR   PRINTS; PR00223; GEMCOATARBR1.
DR   PRINTS; PR00225; GEMCOATBR1.
PE   3: Inferred from homology;
KW   DNA-binding; Host cell membrane; Host cytoplasm; Host membrane;
KW   Host nucleus; Host-virus interaction; Membrane; Reference proteome;
KW   Transport; Viral movement protein.
FT   CHAIN           1..256
FT                   /note="Nuclear shuttle protein"
FT                   /id="PRO_0000222268"
FT   REGION          150..187
FT                   /note="Interaction with Arabidopsis thaliana NSI protein"
FT                   /evidence="ECO:0000250"
FT   MOTIF           21..42
FT                   /note="Bipartite nuclear localization signal"
FT                   /evidence="ECO:0000250"
FT   MOTIF           81..96
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   256 AA;  29375 MW;  4DB486E0C396F949 CRC64;
     MYSTKYRRGF LANQGRGYPR HSTGKRSRNV SRIDFKRRSS KYVHGNDDSK MANQRIHENQ
     FGPEFVMVHN TAISTFITFP SLGKTEPSRS RSYIKLKRLR FKGTVKIERV HVDLSMDGPS
     PKIEGVFSLV VVVDRQPHLS PTGCLHTFDE LFGARIHSHG NLAVSSALKD RFYIRHVFKR
     VISVEKDSTM IDLEGMTSFT NRRFNCWSAF KDFDRQACNG VYGNISKNAI LVYYCWMSDI
     VSKASTFVSF DLDYVG