ID   NSP_TMOV                Reviewed;         257 AA.
AC   Q06661;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   02-JUN-2021, entry version 75.
DE   RecName: Full=Nuclear shuttle protein;
DE            Short=NSP;
DE   AltName: Full=Protein BR1;
DE   AltName: Full=Protein BV1;
GN   ORFNames=BR1, BV1;
OS   Tomato mottle virus (isolate Florida) (ToMoV).
OC   Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes;
OC   Geplafuvirales; Geminiviridae; Begomovirus.
OX   NCBI_TaxID=223359;
OH   NCBI_TaxID=4097; Nicotiana tabacum (Common tobacco).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1469361; DOI=10.1099/0022-1317-73-12-3225;
RA   Abouzid A.M., Polston J.E., Hiebert E.;
RT   "The nucleotide sequence of tomato mottle virus, a new geminivirus isolated
RT   from tomatoes in Florida.";
RL   J. Gen. Virol. 73:3225-3229(1992).
CC   -!- FUNCTION: Binds to the genomic viral ssDNA, shuttles it into and out of
CC       the cell nucleus. Begomoviruses use 2 proteins to transport their DNA
CC       from cell to cell. The nuclear shuttle protein (NSP) shuttles it
CC       between nucleus and cytoplasm and the movement protein (MP) probably
CC       transports the DNA-NSP complex to the cell periphery and facilitates
CC       movement across the cell wall (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Binds to single-stranded and double-stranded viral DNA.
CC       Interacts with the host nuclear shuttle interacting (NSI) protein. This
CC       interaction may allow NSP to recruit NSI monomers to the viral genome
CC       and thus regulate nuclear export of viral genome by NSP (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000250}. Host cytoplasm
CC       {ECO:0000250}. Host cell membrane {ECO:0000250}; Peripheral membrane
CC       protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}.
CC       Note=Translocated to the plasma membrane by the movement protein BC1.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the begomovirus nuclear shuttle protein family.
CC       {ECO:0000305}.
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DR   EMBL; L14461; AAC32418.1; -; Genomic_DNA.
DR   PIR; JQ1873; JQ1873.
DR   RefSeq; NP_047253.1; NC_001939.1.
DR   GeneID; 956413; -.
DR   KEGG; vg:956413; -.
DR   Proteomes; UP000008249; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019028; C:viral capsid; IEA:InterPro.
DR   GO; GO:0003697; F:single-stranded DNA binding; IEA:InterPro.
DR   GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR   GO; GO:0051027; P:DNA transport; IEA:InterPro.
DR   GO; GO:0046740; P:transport of virus in host, cell to cell; IEA:UniProtKB-KW.
DR   InterPro; IPR001530; Gemini_BR1.
DR   InterPro; IPR000263; GV_A/BR1_coat.
DR   Pfam; PF00844; Gemini_coat; 1.
DR   PRINTS; PR00223; GEMCOATARBR1.
DR   PRINTS; PR00225; GEMCOATBR1.
PE   3: Inferred from homology;
KW   DNA-binding; Host cell membrane; Host cytoplasm; Host membrane;
KW   Host nucleus; Host-virus interaction; Membrane; Reference proteome;
KW   Transport; Viral movement protein.
FT   CHAIN           1..257
FT                   /note="Nuclear shuttle protein"
FT                   /id="PRO_0000222269"
FT   REGION          26..47
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          151..188
FT                   /note="Interaction with Arabidopsis thaliana NSI protein"
FT                   /evidence="ECO:0000250"
FT   MOTIF           21..42
FT                   /note="Bipartite nuclear localization signal"
FT                   /evidence="ECO:0000250"
FT   MOTIF           81..97
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000250"
FT   COMPBIAS        27..47
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   257 AA;  29508 MW;  0C297D2BB04D1566 CRC64;
     MYPLKSKRGF SYSNRRFNSR NIVFNRPVSG KRHDGKRRGG NFVKPNDEPK MLAQRIHENQ
     YGPEFVLAHN SAISTFIPVI HLGQVPKPSR SRSYIKLKRL RFKGTVKIER VQSDLNMDGF
     MPKVEGVFSM VVVVDRKPHL GPSGCLHTFD ELFGARINSH GNLTIVPSLK DRFYIRHVFK
     RVLSVEKDTL MVDVEGSTTL SNRRYNCWST FKDLDRESCK GVYDNISKNA LLVYYCWMSD
     TPANASSFVS FDLDYIG