NSRA_ARATH
ID NSRA_ARATH Reviewed; 233 AA.
AC A1A6K6; F4I435; O49280; Q8GYL1;
DT 14-OCT-2015, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Nuclear speckle RNA-binding protein A {ECO:0000303|PubMed:25073154};
DE Short=AtNSRA {ECO:0000303|PubMed:25073154};
GN Name=NSRA {ECO:0000303|PubMed:25073154};
GN OrderedLocusNames=At1g76940 {ECO:0000312|Araport:AT1G76940};
GN ORFNames=F22K20.4 {ECO:0000312|EMBL:AAC00617.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:ABL66782.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Bautista V.R., Kim C.J., Chen H., Quinitio C., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 61-233.
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=25073154; DOI=10.1016/j.devcel.2014.06.017;
RA Bardou F., Ariel F., Simpson C.G., Romero-Barrios N., Laporte P.,
RA Balzergue S., Brown J.W., Crespi M.;
RT "Long noncoding RNA modulates alternative splicing regulators in
RT Arabidopsis.";
RL Dev. Cell 30:166-176(2014).
CC -!- FUNCTION: Alternative splicing (AS) regulator that binds to specific
CC mRNAs and modulates auxin effects on the transcriptome. Displaced from
CC its targets upon binding to AS competitor long non-coding RNA (ASCO-
CC RNA). {ECO:0000269|PubMed:25073154}.
CC -!- SUBCELLULAR LOCATION: Nucleus speckle {ECO:0000269|PubMed:25073154}.
CC Note=Relocalizes to cytoplasmic bodies in trichoblast cells containing
CC root hairs or when coexpressed with Medicago enod40 RNA.
CC {ECO:0000269|PubMed:25073154}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=A1A6K6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A1A6K6-2; Sequence=VSP_057907;
CC -!- TISSUE SPECIFICITY: Expressed in root meristems, lateral root primordia
CC and root vascular tissues. {ECO:0000269|PubMed:25073154}.
CC -!- INDUCTION: Constitutively expressed. {ECO:0000269|PubMed:25073154}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype. Nsra and nsrb double
CC mutants are less sensitive to auxin. {ECO:0000269|PubMed:25073154}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC00617.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAC42194.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC002291; AAC00617.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE35906.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35907.1; -; Genomic_DNA.
DR EMBL; BT029526; ABL66782.1; -; mRNA.
DR EMBL; AK117533; BAC42194.1; ALT_INIT; mRNA.
DR PIR; C96798; C96798.
DR RefSeq; NP_001185414.1; NM_001198485.1. [A1A6K6-2]
DR RefSeq; NP_177820.2; NM_106345.4. [A1A6K6-1]
DR AlphaFoldDB; A1A6K6; -.
DR SMR; A1A6K6; -.
DR STRING; 3702.AT1G76940.1; -.
DR PaxDb; A1A6K6; -.
DR PRIDE; A1A6K6; -.
DR ProteomicsDB; 249072; -. [A1A6K6-1]
DR EnsemblPlants; AT1G76940.1; AT1G76940.1; AT1G76940. [A1A6K6-1]
DR EnsemblPlants; AT1G76940.2; AT1G76940.2; AT1G76940. [A1A6K6-2]
DR GeneID; 844029; -.
DR Gramene; AT1G76940.1; AT1G76940.1; AT1G76940. [A1A6K6-1]
DR Gramene; AT1G76940.2; AT1G76940.2; AT1G76940. [A1A6K6-2]
DR KEGG; ath:AT1G76940; -.
DR Araport; AT1G76940; -.
DR TAIR; locus:2025232; AT1G76940.
DR eggNOG; ENOG502S9VX; Eukaryota.
DR HOGENOM; CLU_078642_1_1_1; -.
DR InParanoid; A1A6K6; -.
DR OMA; AMDEFMM; -.
DR PhylomeDB; A1A6K6; -.
DR PRO; PR:A1A6K6; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; A1A6K6; baseline and differential.
DR Genevisible; A1A6K6; AT.
DR GO; GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF00076; RRM_1; 1.
DR SMART; SM00360; RRM; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Nucleus; Reference proteome; RNA-binding.
FT CHAIN 1..233
FT /note="Nuclear speckle RNA-binding protein A"
FT /id="PRO_0000434146"
FT DOMAIN 136..222
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 1..54
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 68..92
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 214..233
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 28..81
FT /note="Missing (in isoform 2)"
FT /id="VSP_057907"
SQ SEQUENCE 233 AA; 25420 MW; A742C497E76E1FEC CRC64;
MADGYWNQQR QQHHPPGGPM KRPRSDFEAP SSTMTIGHGG GYYPRDEDLD VPDTRTIGSA
YDRYLQSVQS GEGGSVSMGR SGGGGGGGGG NVQTIDDFML RRGGVLPLDH GPNGHTIGFD
PPEPVGRRNL PSDASNTLYV EGLPSNCSRR EVAHIFRPFV GYREVRLVTK DSKHRNGDPI
VLCFVDFTNP ACAATALSAL QGYRMDENES DSKFLRLQFS RKPGSRPGQR GRR
