NSRB_ARATH
ID NSRB_ARATH Reviewed; 421 AA.
AC F4HWF9; F4HWF8; Q9LMP3;
DT 14-OCT-2015, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 77.
DE RecName: Full=Nuclear speckle RNA-binding protein B {ECO:0000303|PubMed:25073154};
DE Short=AtNSRB {ECO:0000303|PubMed:25073154};
GN Name=NSRB {ECO:0000303|PubMed:25073154};
GN OrderedLocusNames=At1g21320 {ECO:0000312|Araport:AT1G21320};
GN ORFNames=F16F4.3 {ECO:0000312|EMBL:AAF81354.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP FUNCTION, ALTERNATIVE SPLICING, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP INDUCTION BY AUXIN, AND DISRUPTION PHENOTYPE.
RX PubMed=25073154; DOI=10.1016/j.devcel.2014.06.017;
RA Bardou F., Ariel F., Simpson C.G., Romero-Barrios N., Laporte P.,
RA Balzergue S., Brown J.W., Crespi M.;
RT "Long noncoding RNA modulates alternative splicing regulators in
RT Arabidopsis.";
RL Dev. Cell 30:166-176(2014).
CC -!- FUNCTION: Alternative splicing (AS) regulator that binds to specific
CC mRNAs and modulates auxin effects on the transcriptome. Displaced from
CC its targets upon binding to AS competitor long non-coding RNA (ASCO-
CC RNA). {ECO:0000269|PubMed:25073154}.
CC -!- SUBCELLULAR LOCATION: Nucleus speckle {ECO:0000269|PubMed:25073154}.
CC Note=Relocalizes to cytoplasmic bodies in trichoblast cells containing
CC root hairs or when coexpressed with Medicago ENOD40 RNA.
CC {ECO:0000269|PubMed:25073154}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative promoter usage; Named isoforms=2;
CC Name=1;
CC IsoId=F4HWF9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F4HWF9-2; Sequence=VSP_057908;
CC -!- TISSUE SPECIFICITY: Isoform 1: Expressed in root meristems, lateral
CC root primordia, root vascular tissues and cotyledon vascular tissues.
CC Isoform 2: Expressed in root meristems, lateral root primordia and root
CC vascular tissues. {ECO:0000269|PubMed:25073154}.
CC -!- INDUCTION: Up-regulated by auxin treatment.
CC {ECO:0000269|PubMed:25073154}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype. Nsra and nsrb double
CC mutants are less sensitive to auxin. {ECO:0000269|PubMed:25073154}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF81354.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC036104; AAF81354.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE30084.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30085.1; -; Genomic_DNA.
DR PIR; C86346; C86346.
DR RefSeq; NP_001154354.1; NM_001160882.2. [F4HWF9-2]
DR RefSeq; NP_173554.3; NM_101984.4. [F4HWF9-1]
DR AlphaFoldDB; F4HWF9; -.
DR SMR; F4HWF9; -.
DR STRING; 3702.AT1G21320.1; -.
DR iPTMnet; F4HWF9; -.
DR PaxDb; F4HWF9; -.
DR PRIDE; F4HWF9; -.
DR EnsemblPlants; AT1G21320.1; AT1G21320.1; AT1G21320. [F4HWF9-1]
DR EnsemblPlants; AT1G21320.2; AT1G21320.2; AT1G21320. [F4HWF9-2]
DR GeneID; 838729; -.
DR Gramene; AT1G21320.1; AT1G21320.1; AT1G21320. [F4HWF9-1]
DR Gramene; AT1G21320.2; AT1G21320.2; AT1G21320. [F4HWF9-2]
DR KEGG; ath:AT1G21320; -.
DR Araport; AT1G21320; -.
DR TAIR; locus:2014937; AT1G21320.
DR HOGENOM; CLU_652744_0_0_1; -.
DR InParanoid; F4HWF9; -.
DR OMA; PMSSIPH; -.
DR OrthoDB; 1252072at2759; -.
DR PRO; PR:F4HWF9; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4HWF9; baseline and differential.
DR Genevisible; F4HWF9; AT.
DR GO; GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR InterPro; IPR008889; VQ.
DR Pfam; PF00076; RRM_1; 1.
DR Pfam; PF05678; VQ; 1.
DR SMART; SM00360; RRM; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 1.
PE 2: Evidence at transcript level;
KW Alternative promoter usage; Nucleus; Reference proteome; RNA-binding.
FT CHAIN 1..421
FT /note="Nuclear speckle RNA-binding protein B"
FT /id="PRO_0000434147"
FT DOMAIN 323..409
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 1..64
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 86..114
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 197..226
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 32..63
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..201
FT /note="MDNRSPRSRGNMGPKPTPLKVRGDSHKIIKKPPLAPPHPQPQPPQTHQQEPS
FT QSRPPPGPVNIYTVTPRIIHTHPNNFMTLVQRLTGQTSTSTTSSSSSSSTSEPKDTSTM
FT VDTSHGLISPAARFAVTEKANISNELGTFVGGEGTMDQYYHHHHQEQPHQNRGFERPSF
FT HHAGILSPGPYSLPSVSPDFFSTIGPTDPQE -> MADEYWNQQRQYQLPISSNPHVLP
FT PKRPRSDFQ (in isoform 2)"
FT /id="VSP_057908"
SQ SEQUENCE 421 AA; 46158 MW; FB48F86CD1F1F0AC CRC64;
MDNRSPRSRG NMGPKPTPLK VRGDSHKIIK KPPLAPPHPQ PQPPQTHQQE PSQSRPPPGP
VNIYTVTPRI IHTHPNNFMT LVQRLTGQTS TSTTSSSSSS STSEPKDTST MVDTSHGLIS
PAARFAVTEK ANISNELGTF VGGEGTMDQY YHHHHQEQPH QNRGFERPSF HHAGILSPGP
YSLPSVSPDF FSTIGPTDPQ EGTPYLIPSG DMHSYLSQDE DRGIPHSVKD TRSIGSAYDR
YLQSMQTFFV PSEEAGPFNG VGMVRQGGSN MMPGPSMGEL MAGCGGSLPS DFRPNGRDMG
FGQLDSVGRP GREPHPLPPD VSNTLYVEGL PSNCSRREVS HIFRPFVGYR EVRLVTQDSK
HRSGDPTVLC FVDFENSACA ATALSALQDY RMDEDEPDSK ILRLQFFRNP GPRPGQRGGR
R
