ID   NSRF_ASPN1              Reviewed;         433 AA.
AC   A0A2I1C3U2;
DT   29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT   28-FEB-2018, sequence version 1.
DT   23-FEB-2022, entry version 11.
DE   RecName: Full=Baeyer-Villiger monooxygenase nsrF {ECO:0000303|PubMed:30394754};
DE            Short=BVMO nsrF {ECO:0000303|PubMed:30394754};
DE            EC=1.-.-.- {ECO:0000269|PubMed:32105084, ECO:0000269|PubMed:33891392};
DE   AltName: Full=Neosartorin biosynthesis cluster protein F {ECO:0000303|PubMed:30394754};
GN   Name=nsrF {ECO:0000303|PubMed:30394754}; ORFNames=P174DRAFT_512934;
OS   Aspergillus novofumigatus (strain IBT 16806).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus.
OX   NCBI_TaxID=1392255;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IBT 16806;
RX   PubMed=29317534; DOI=10.1073/pnas.1715954115;
RA   Kjaerboelling I., Vesth T.C., Frisvad J.C., Nybo J.L., Theobald S., Kuo A.,
RA   Bowyer P., Matsuda Y., Mondo S., Lyhne E.K., Kogle M.E., Clum A.,
RA   Lipzen A., Salamov A., Ngan C.Y., Daum C., Chiniquy J., Barry K.,
RA   LaButti K., Haridas S., Simmons B.A., Magnuson J.K., Mortensen U.H.,
RA   Larsen T.O., Grigoriev I.V., Baker S.E., Andersen M.R.;
RT   "Linking secondary metabolites to gene clusters through genome sequencing
RT   of six diverse Aspergillus species.";
RL   Proc. Natl. Acad. Sci. U.S.A. 115:E753-E761(2018).
RN   [2]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND PATHWAY.
RX   PubMed=30394754; DOI=10.1021/acs.orglett.8b03123;
RA   Matsuda Y., Gotfredsen C.H., Larsen T.O.;
RT   "Genetic characterization of neosartorin biosynthesis provides insight into
RT   heterodimeric natural product generation.";
RL   Org. Lett. 20:7197-7200(2018).
RN   [3]
RP   FUNCTION, CATALYTIC ACTIVITY, AND PATHWAY.
RX   PubMed=32105084; DOI=10.1021/acs.orglett.0c00285;
RA   Wei X., Matsuda Y.;
RT   "Unraveling the fungal strategy for tetrahydroxanthone biosynthesis and
RT   diversification.";
RL   Org. Lett. 22:1919-1923(2020).
RN   [4]
RP   FUNCTION, CATALYTIC ACTIVITY, AND PATHWAY.
RX   PubMed=33891392; DOI=10.1021/acs.jnatprod.1c00022;
RA   Wei X., Chen X., Chen L., Yan D., Wang W.G., Matsuda Y.;
RT   "Heterologous biosynthesis of tetrahydroxanthone dimers: determination of
RT   key factors for selective or divergent synthesis.";
RL   J. Nat. Prod. 84:1544-1549(2021).
CC   -!- FUNCTION: Baeyer-Villiger monooxygenase; part of the gene cluster that
CC       mediates the biosynthesis of the tetrahydroxanthone dimer neosartorin,
CC       which exhibits antibacterial activity (PubMed:30394754,
CC       PubMed:32105084, PubMed:33891392). The two different monomeric units
CC       appear to be synthesized by the same set of enzymes, among which the
CC       Baeyer-Villiger monooxygenase nsrF is the key enzyme for the divergence
CC       of the biosynthetic routes (PubMed:32105084). The pathway begins with
CC       the synthesis of atrochrysone thioester by the polyketide synthase nsrB
CC       (PubMed:32105084). The atrochrysone carboxyl ACP thioesterase nsrC then
CC       breaks the thioester bond and releases the atrochrysone carboxylic acid
CC       from AacuL (PubMed:32105084). Atrochrysone carboxylic acid is
CC       decarboxylated by the decarboxylase nsrE, and oxidized by the anthrone
CC       oxygenase nsrD to yield emodin (PubMed:32105084). Emodin is then
CC       reduced to emodin hydroquinone by the oxidoreductase nsrR
CC       (PubMed:32105084). A-ring reduction by the short chain dehydrogenase
CC       nsrJ, dehydration by the scytalone dehydratase-like protein nsrI and
CC       probable spontaneous re-oxidation, results in overall deoxygenation to
CC       chrysophanol (PubMed:32105084). The Baeyer-Villiger monooxygenase nsrF
CC       accepts chrysophanol as a substrate to insert one oxygen atom at two
CC       different positions to yield the precursors of both monomric units
CC       (PubMed:30394754, PubMed:32105084, PubMed:33891392). NsrF is
CC       promiscuous/flexible in interacting with the 2 (non methylated and
CC       methylated) aromatic rings of chrysophanol, thus diverging the
CC       biosynthetic pathway at this point (PubMed:30394754, PubMed:32105084,
CC       PubMed:33891392). After the hydrolysis of the lactones,
CC       methylesterification by the methyltransferase nsrG yields respectively
CC       moniliphenone and 2,2',6'-trihydroxy-4-methyl-6-methoxya-
CC       cyldiphenylmethanone (PubMed:30394754, PubMed:32105084). The next steps
CC       are the hydroxylation by the FAD-dependent monooxygenase nsrK, followed
CC       by isomerization by the monooxygenase nsrQ (PubMed:32105084). The short
CC       chain dehydrogenase/reductase nsrO then catalyzes the C-5 ketoreduction
CC       to give the xanthone skeleton of blennolide C and 5-acetylblennolide A
CC       (PubMed:32105084). The acetyltransferase nsrL has a strict substrate
CC       specificity and uses only blennolide A but not blennolide C to yield 5-
CC       acetylblennolide A as the single-acetylated product (PubMed:30394754).
CC       In the final step of the biosynthesis, the heterodimerization of the 2
CC       xanthones, blennolide C and 5-acetylblennolide A, is catalyzed by the
CC       cytochrome P450 monooxygenase nsrP (PubMed:30394754). NsrP can utilize
CC       at least three different xanthones as its substrates to perform the
CC       dimerization reaction (PubMed:30394754). {ECO:0000269|PubMed:30394754,
CC       ECO:0000269|PubMed:32105084, ECO:0000269|PubMed:33891392}.
CC   -!- PATHWAY: Secondary metabolite biosynthesis.
CC       {ECO:0000269|PubMed:30394754, ECO:0000269|PubMed:33891392}.
CC   -!- DISRUPTION PHENOTYPE: Does not completely abolish the neosartorin
CC       synthesis, but accumulates chrysophanol. {ECO:0000269|PubMed:30394754}.
CC   -!- SIMILARITY: Belongs to the AflY oxidoreductase family. {ECO:0000305}.
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DR   EMBL; MSZS01000005; PKX92304.1; -; Genomic_DNA.
DR   STRING; 1392255.A0A2I1C3U2; -.
DR   VEuPathDB; FungiDB:P174DRAFT_512934; -.
DR   OrthoDB; 614605at2759; -.
DR   Proteomes; UP000234474; Unassembled WGS sequence.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   InterPro; IPR025337; Questin_oxidase-like.
DR   PANTHER; PTHR35870; PTHR35870; 2.
DR   Pfam; PF14027; Questin_oxidase; 1.
PE   1: Evidence at protein level;
KW   Oxidoreductase; Reference proteome.
FT   CHAIN           1..433
FT                   /note="Baeyer-Villiger monooxygenase nsrF"
FT                   /id="PRO_0000453473"
SQ   SEQUENCE   433 AA;  48569 MW;  1D45546B31B8C070 CRC64;
     MTTTTIEAPA IAGFHLSPEK VLGQVEPYSP GATDAASTLL QQNHEKWHMY FRDVAGHNHI
     PHAILTALPP MEAPTVGKMR DPAVFRQHMQ RLDQYSNFLA FFEQELEQPG SSKEAVVSRY
     CFSRTPLADF MLAQLFEGLY HPIIHLGFGI EFHLPGLVAE GLAQAASHDP MKIDVFFQAA
     EALAGTGSLA SKPLVELYRQ VRASDTLRTA ARLSDGPWKV RDGVLGRAFD EIVNVAAQFQ
     VSSNEHDVQR ATVEMMSCAA WACGGPHRPG KERKIDFFLM HCVTSSIFFT VLGRATWISI
     ADKARLVEWK ARLDLVWYAA CAAPELRDEN FLNYEPTLSR GMDWRQLYHA LNRYHDDGHV
     AKFVRALKNA EEVALPFEQE HADILPVKGD AWFRIAQICY DSTASHTEAQ KKWVWGVGFA
     PMWDLVPDFV TSS