NSRG_ASPN1
ID NSRG_ASPN1 Reviewed; 261 AA.
AC A0A2I1C3X9;
DT 29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT 28-FEB-2018, sequence version 1.
DT 03-AUG-2022, entry version 12.
DE RecName: Full=Methyltransferase nsrG {ECO:0000303|PubMed:30394754};
DE EC=2.1.1.- {ECO:0000269|PubMed:32105084, ECO:0000269|PubMed:33891392};
DE AltName: Full=Neosartorin biosynthesis cluster protein G {ECO:0000303|PubMed:30394754};
GN Name=nsrG {ECO:0000303|PubMed:30394754}; ORFNames=P174DRAFT_512933;
OS Aspergillus novofumigatus (strain IBT 16806).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus.
OX NCBI_TaxID=1392255;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IBT 16806;
RX PubMed=29317534; DOI=10.1073/pnas.1715954115;
RA Kjaerboelling I., Vesth T.C., Frisvad J.C., Nybo J.L., Theobald S., Kuo A.,
RA Bowyer P., Matsuda Y., Mondo S., Lyhne E.K., Kogle M.E., Clum A.,
RA Lipzen A., Salamov A., Ngan C.Y., Daum C., Chiniquy J., Barry K.,
RA LaButti K., Haridas S., Simmons B.A., Magnuson J.K., Mortensen U.H.,
RA Larsen T.O., Grigoriev I.V., Baker S.E., Andersen M.R.;
RT "Linking secondary metabolites to gene clusters through genome sequencing
RT of six diverse Aspergillus species.";
RL Proc. Natl. Acad. Sci. U.S.A. 115:E753-E761(2018).
RN [2]
RP FUNCTION, DISRUPTION PHENOTYPE, AND PATHWAY.
RX PubMed=30394754; DOI=10.1021/acs.orglett.8b03123;
RA Matsuda Y., Gotfredsen C.H., Larsen T.O.;
RT "Genetic characterization of neosartorin biosynthesis provides insight into
RT heterodimeric natural product generation.";
RL Org. Lett. 20:7197-7200(2018).
RN [3]
RP FUNCTION, CATALYTIC ACTIVITY, AND PATHWAY.
RX PubMed=32105084; DOI=10.1021/acs.orglett.0c00285;
RA Wei X., Matsuda Y.;
RT "Unraveling the fungal strategy for tetrahydroxanthone biosynthesis and
RT diversification.";
RL Org. Lett. 22:1919-1923(2020).
RN [4]
RP FUNCTION, CATALYTIC ACTIVITY, AND PATHWAY.
RX PubMed=33891392; DOI=10.1021/acs.jnatprod.1c00022;
RA Wei X., Chen X., Chen L., Yan D., Wang W.G., Matsuda Y.;
RT "Heterologous biosynthesis of tetrahydroxanthone dimers: determination of
RT key factors for selective or divergent synthesis.";
RL J. Nat. Prod. 84:1544-1549(2021).
CC -!- FUNCTION: Methyltransferase; part of the gene cluster that mediates the
CC biosynthesis of the tetrahydroxanthone dimer neosartorin, which
CC exhibits antibacterial activity (PubMed:30394754, PubMed:32105084,
CC PubMed:33891392). The two different monomeric units appear to be
CC synthesized by the same set of enzymes, among which the Baeyer-Villiger
CC monooxygenase nsrF is the key enzyme for the divergence of the
CC biosynthetic routes (PubMed:32105084). The pathway begins with the
CC synthesis of atrochrysone thioester by the polyketide synthase nsrB
CC (PubMed:32105084). The atrochrysone carboxyl ACP thioesterase nsrC then
CC breaks the thioester bond and releases the atrochrysone carboxylic acid
CC from AacuL (PubMed:32105084). Atrochrysone carboxylic acid is
CC decarboxylated by the decarboxylase nsrE, and oxidized by the anthrone
CC oxygenase nsrD to yield emodin (PubMed:32105084). Emodin is then
CC reduced to emodin hydroquinone by the oxidoreductase nsrR
CC (PubMed:32105084). A-ring reduction by the short chain dehydrogenase
CC nsrJ, dehydration by the scytalone dehydratase-like protein nsrI and
CC probable spontaneous re-oxidation, results in overall deoxygenation to
CC chrysophanol (PubMed:32105084). The Baeyer-Villiger monooxygenase nsrF
CC accepts chrysophanol as a substrate to insert one oxygen atom at two
CC different positions to yield the precursors of both monomric units
CC (PubMed:30394754, PubMed:32105084, PubMed:33891392). NsrF is
CC promiscuous/flexible in interacting with the 2 (non methylated and
CC methylated) aromatic rings of chrysophanol, thus diverging the
CC biosynthetic pathway at this point (PubMed:30394754, PubMed:32105084,
CC PubMed:33891392). After the hydrolysis of the lactones,
CC methylesterification by the methyltransferase nsrG yields respectively
CC moniliphenone and 2,2',6'-trihydroxy-4-methyl-6-methoxya-
CC cyldiphenylmethanone (PubMed:30394754, PubMed:32105084). The next steps
CC are the hydroxylation by the FAD-dependent monooxygenase nsrK, followed
CC by isomerization by the monooxygenase nsrQ (PubMed:32105084). The short
CC chain dehydrogenase/reductase nsrO then catalyzes the C-5 ketoreduction
CC to give the xanthone skeleton of blennolide C and 5-acetylblennolide A
CC (PubMed:32105084). The acetyltransferase nsrL has a strict substrate
CC specificity and uses only blennolide A but not blennolide C to yield 5-
CC acetylblennolide A as the single-acetylated product (PubMed:30394754).
CC In the final step of the biosynthesis, the heterodimerization of the 2
CC xanthones, blennolide C and 5-acetylblennolide A, is catalyzed by the
CC cytochrome P450 monooxygenase nsrP (PubMed:30394754). NsrP can utilize
CC at least three different xanthones as its substrates to perform the
CC dimerization reaction (PubMed:30394754). {ECO:0000269|PubMed:30394754,
CC ECO:0000269|PubMed:32105084, ECO:0000269|PubMed:33891392}.
CC -!- PATHWAY: Secondary metabolite biosynthesis.
CC {ECO:0000269|PubMed:30394754, ECO:0000269|PubMed:32105084,
CC ECO:0000269|PubMed:33891392}.
CC -!- DISRUPTION PHENOTYPE: Impairs the production of neosartorin but
CC accumulates no metabolites related to the neosartorin pathway.
CC {ECO:0000269|PubMed:30394754}.
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily.
CC {ECO:0000305}.
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DR EMBL; MSZS01000005; PKX92303.1; -; Genomic_DNA.
DR SMR; A0A2I1C3X9; -.
DR STRING; 1392255.A0A2I1C3X9; -.
DR VEuPathDB; FungiDB:P174DRAFT_512933; -.
DR OrthoDB; 899118at2759; -.
DR Proteomes; UP000234474; Unassembled WGS sequence.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR013216; Methyltransf_11.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR Pfam; PF08241; Methyltransf_11; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
PE 1: Evidence at protein level;
KW Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW Transferase.
FT CHAIN 1..261
FT /note="Methyltransferase nsrG"
FT /id="PRO_0000453496"
FT REGION 49..141
FT /note="Methyltransferase domain"
FT /evidence="ECO:0000255"
SQ SEQUENCE 261 AA; 29410 MW; E2BE0BC000788BAA CRC64;
MEVNDKFFAQ DDIFWENYLK GRPRVPDSFF DRIFDYHKAK GGHFGTVHDV GAGNGPYALR
LRSRFDHVIV SDIVANNIEL ARRRLQGEEG FSFRTSRLQD ADDIAAGSVD MVFATNVMHF
ADPQDIAMAA IAHQLRPGAL FRQGGRQLLN KANDPTETAR VMLRTQGTRG IQSMLPLDDV
HQTTPEPNYT GPDDAEIYED DEGWSFETDL AGVKEHFESF PFVSQFPGAF TEMYRELDDL
LADGKPVQGY FPVKVILATR S
