NSRR_BACSU
ID NSRR_BACSU Reviewed; 146 AA.
AC O07573;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=HTH-type transcriptional regulator NsrR;
GN Name=nsrR; Synonyms=yhdE; OrderedLocusNames=BSU09380;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9579061; DOI=10.1099/00221287-144-4-859;
RA Noback M.A., Holsappel S., Kiewiet R., Terpstra P., Wambutt R., Wedler H.,
RA Venema G., Bron S.;
RT "The 172 kb prkA-addAB region from 83 degrees to 97 degrees of the Bacillus
RT subtilis chromosome contains several dysfunctional genes, the glyB marker,
RT many genes encoding transporter proteins, and the ubiquitous hit gene.";
RL Microbiology 144:859-875(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP FUNCTION.
RC STRAIN=168 / JH642;
RX PubMed=16885456; DOI=10.1128/jb.00486-06;
RA Nakano M.M., Geng H., Nakano S., Kobayashi K.;
RT "The nitric oxide-responsive regulator NsrR controls ResDE-dependent gene
RT expression.";
RL J. Bacteriol. 188:5878-5887(2006).
CC -!- FUNCTION: Nitric oxide-responsive transcriptional regulator. It
CC represses the expression of flavohemoprotein hmp and the nitrite
CC reductase nasD. Probably plays a role in the up-regulation of the resDE
CC regulon in the presence of nitric oxide. {ECO:0000269|PubMed:16885456}.
CC -!- COFACTOR:
CC Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135; Evidence={ECO:0000305};
CC Note=Binds 1 [2Fe-2S] cluster per subunit. {ECO:0000305};
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DR EMBL; Y14082; CAA74483.1; -; Genomic_DNA.
DR EMBL; Y14079; CAA74438.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB12777.1; -; Genomic_DNA.
DR PIR; C69825; C69825.
DR RefSeq; NP_388819.1; NC_000964.3.
DR RefSeq; WP_003245378.1; NZ_JNCM01000035.1.
DR AlphaFoldDB; O07573; -.
DR SMR; O07573; -.
DR IntAct; O07573; 1.
DR STRING; 224308.BSU09380; -.
DR PaxDb; O07573; -.
DR PRIDE; O07573; -.
DR DNASU; 939744; -.
DR EnsemblBacteria; CAB12777; CAB12777; BSU_09380.
DR GeneID; 939744; -.
DR KEGG; bsu:BSU09380; -.
DR PATRIC; fig|224308.179.peg.1011; -.
DR eggNOG; COG1959; Bacteria.
DR InParanoid; O07573; -.
DR OMA; AQEAFYA; -.
DR PhylomeDB; O07573; -.
DR BioCyc; BSUB:BSU09380-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR030489; TR_Rrf2-type_CS.
DR InterPro; IPR000944; Tscrpt_reg_Rrf2.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR33221; PTHR33221; 1.
DR Pfam; PF02082; Rrf2; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR TIGRFAMs; TIGR00738; rrf2_super; 1.
DR PROSITE; PS01332; HTH_RRF2_1; 1.
DR PROSITE; PS51197; HTH_RRF2_2; 1.
PE 4: Predicted;
KW 2Fe-2S; Activator; DNA-binding; Iron; Iron-sulfur; Metal-binding;
KW Reference proteome; Repressor; Transcription; Transcription regulation.
FT CHAIN 1..146
FT /note="HTH-type transcriptional regulator NsrR"
FT /id="PRO_0000109567"
FT DOMAIN 2..133
FT /note="HTH rrf2-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00540"
FT DNA_BIND 28..51
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00540"
FT BINDING 92
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00540"
FT BINDING 100
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00540"
FT BINDING 106
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00540"
SQ SEQUENCE 146 AA; 16609 MW; 02C519057F1A3A9C CRC64;
MKLTNYTDYS LRVLIFLAAE RPGELSNIKQ IAETYSISKN HLMKVIYRLG QLGYVETIRG
RGGGIRLGMD PEDINIGEVV RKTEDDFNIV ECFDANKNLC VISPVCGLKH VLNEALLAYL
AVLDKYTLRD LVKNKEDIMK LLKMKE
