ID   NSRR_ECO5E              Reviewed;         141 AA.
AC   B5Z2I4;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   25-NOV-2008, sequence version 1.
DT   03-AUG-2022, entry version 72.
DE   RecName: Full=HTH-type transcriptional repressor NsrR {ECO:0000255|HAMAP-Rule:MF_01177};
GN   Name=nsrR {ECO:0000255|HAMAP-Rule:MF_01177};
GN   OrderedLocusNames=ECH74115_5694;
OS   Escherichia coli O157:H7 (strain EC4115 / EHEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=444450;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=EC4115 / EHEC;
RX   PubMed=22135463; DOI=10.1073/pnas.1107176108;
RA   Eppinger M., Mammel M.K., Leclerc J.E., Ravel J., Cebula T.A.;
RT   "Genomic anatomy of Escherichia coli O157:H7 outbreaks.";
RL   Proc. Natl. Acad. Sci. U.S.A. 108:20142-20147(2011).
CC   -!- FUNCTION: Nitric oxide-sensitive repressor of genes involved in
CC       protecting the cell against nitrosative stress. May require iron for
CC       activity. {ECO:0000255|HAMAP-Rule:MF_01177}.
CC   -!- COFACTOR:
CC       Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01177};
CC       Note=Binds 1 [2Fe-2S] cluster per subunit. {ECO:0000255|HAMAP-
CC       Rule:MF_01177};
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DR   EMBL; CP001164; ACI36234.1; -; Genomic_DNA.
DR   RefSeq; WP_001177633.1; NC_011353.1.
DR   AlphaFoldDB; B5Z2I4; -.
DR   SMR; B5Z2I4; -.
DR   KEGG; ecf:ECH74115_5694; -.
DR   HOGENOM; CLU_107144_2_1_6; -.
DR   OMA; AQEAFYA; -.
DR   GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 1.10.10.10; -; 1.
DR   HAMAP; MF_01177; HTH_type_NsrR; 1.
DR   InterPro; IPR030489; TR_Rrf2-type_CS.
DR   InterPro; IPR000944; Tscrpt_reg_Rrf2.
DR   InterPro; IPR023761; Tscrpt_rep_HTH_NsrR.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR33221; PTHR33221; 1.
DR   Pfam; PF02082; Rrf2; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   TIGRFAMs; TIGR00738; rrf2_super; 1.
DR   PROSITE; PS01332; HTH_RRF2_1; 1.
DR   PROSITE; PS51197; HTH_RRF2_2; 1.
PE   3: Inferred from homology;
KW   2Fe-2S; DNA-binding; Iron; Iron-sulfur; Metal-binding; Repressor;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..141
FT                   /note="HTH-type transcriptional repressor NsrR"
FT                   /id="PRO_1000138116"
FT   DOMAIN          2..129
FT                   /note="HTH rrf2-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01177"
FT   DNA_BIND        28..51
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01177"
FT   BINDING         91
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01177"
FT   BINDING         96
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01177"
FT   BINDING         102
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01177"
SQ   SEQUENCE   141 AA;  15550 MW;  3A78385E0239EFAC CRC64;
     MQLTSFTDYG LRALIYMASL PEGLMTSISE VTDVYGVSRN HMVKIINQLS RAGYVTAVRG
     KNGGIRLGKP ASAIRIGDVV RELEPLSLVN CSSEFCHITP ACRLKQALSK AVQSFLTELD
     NYTLADLVEE NQPLYKLLLV E