NSRR_ECOLC
ID NSRR_ECOLC Reviewed; 141 AA.
AC B1IT28;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 29-APR-2008, sequence version 1.
DT 03-AUG-2022, entry version 80.
DE RecName: Full=HTH-type transcriptional repressor NsrR {ECO:0000255|HAMAP-Rule:MF_01177};
GN Name=nsrR {ECO:0000255|HAMAP-Rule:MF_01177}; OrderedLocusNames=EcolC_3835;
OS Escherichia coli (strain ATCC 8739 / DSM 1576 / NBRC 3972 / NCIMB 8545 /
OS WDCM 00012 / Crooks).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=481805;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 8739 / DSM 1576 / NBRC 3972 / NCIMB 8545 / WDCM 00012 / Crooks;
RA Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C.,
RA Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA Mikhailova N., Ingram L., Richardson P.;
RT "Complete sequence of Escherichia coli C str. ATCC 8739.";
RL Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Nitric oxide-sensitive repressor of genes involved in
CC protecting the cell against nitrosative stress. May require iron for
CC activity. {ECO:0000255|HAMAP-Rule:MF_01177}.
CC -!- COFACTOR:
CC Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01177};
CC Note=Binds 1 [2Fe-2S] cluster per subunit. {ECO:0000255|HAMAP-
CC Rule:MF_01177};
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DR EMBL; CP000946; ACA79439.1; -; Genomic_DNA.
DR RefSeq; WP_001177644.1; NZ_CP022959.1.
DR AlphaFoldDB; B1IT28; -.
DR SMR; B1IT28; -.
DR KEGG; ecl:EcolC_3835; -.
DR HOGENOM; CLU_107144_2_1_6; -.
DR OMA; AQEAFYA; -.
DR GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEA:InterPro.
DR Gene3D; 1.10.10.10; -; 1.
DR HAMAP; MF_01177; HTH_type_NsrR; 1.
DR InterPro; IPR030489; TR_Rrf2-type_CS.
DR InterPro; IPR000944; Tscrpt_reg_Rrf2.
DR InterPro; IPR023761; Tscrpt_rep_HTH_NsrR.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR33221; PTHR33221; 1.
DR Pfam; PF02082; Rrf2; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR TIGRFAMs; TIGR00738; rrf2_super; 1.
DR PROSITE; PS01332; HTH_RRF2_1; 1.
DR PROSITE; PS51197; HTH_RRF2_2; 1.
PE 3: Inferred from homology;
KW 2Fe-2S; DNA-binding; Iron; Iron-sulfur; Metal-binding; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..141
FT /note="HTH-type transcriptional repressor NsrR"
FT /id="PRO_1000085433"
FT DOMAIN 2..129
FT /note="HTH rrf2-type"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01177"
FT DNA_BIND 28..51
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01177"
FT BINDING 91
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01177"
FT BINDING 96
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01177"
FT BINDING 102
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01177"
SQ SEQUENCE 141 AA; 15583 MW; C6B73B8CCD3134D5 CRC64;
MQLTSFTDYG LRALIYMASL PEGRMTSISE VTDVYGVSRN HMVKIINQLS RAGYVTAVRG
KNGGIRLGKS ASAIRIGDVV RELEPLSLVN CSSEFCHITP ACRLKQALSK AVQSFLTELD
NYTLADLVEE NQPLYKLLLV E
