NSRR_ECOLI
ID NSRR_ECOLI Reviewed; 141 AA.
AC P0AF63; P21498; P72442; Q2M6C7;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=HTH-type transcriptional repressor NsrR;
GN Name=nsrR; Synonyms=yjeB; OrderedLocusNames=b4178, JW4136;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=3058695; DOI=10.1016/s0021-9258(18)37402-7;
RA Wolfe S.A., Smith J.M.;
RT "Nucleotide sequence and analysis of the purA gene encoding
RT adenylosuccinate synthetase of Escherichia coli K12.";
RL J. Biol. Chem. 263:19147-19153(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=7610040; DOI=10.1093/nar/23.12.2105;
RA Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.;
RT "Analysis of the Escherichia coli genome VI: DNA sequence of the region
RT from 92.8 through 100 minutes.";
RL Nucleic Acids Res. 23:2105-2119(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP FUNCTION.
RC STRAIN=K12 / MC1000 / ATCC 39531;
RX PubMed=16428390; DOI=10.1128/jb.188.3.874-881.2006;
RA Bodenmiller D.M., Spiro S.;
RT "The yjeB (nsrR) gene of Escherichia coli encodes a nitric oxide-sensitive
RT transcriptional regulator.";
RL J. Bacteriol. 188:874-881(2006).
CC -!- FUNCTION: Nitric oxide-sensitive repressor of genes involved in
CC protecting the cell against nitrosative stress, such as ytfE, hmpA and
CC ygbA. May require iron for activity. Does not regulates its own
CC transcription. {ECO:0000269|PubMed:16428390}.
CC -!- COFACTOR:
CC Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135; Evidence={ECO:0000305};
CC Note=Binds 1 [2Fe-2S] cluster per subunit. {ECO:0000305};
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DR EMBL; J04199; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; U14003; AAA97074.1; -; Genomic_DNA.
DR EMBL; U00096; AAC77135.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE78179.1; -; Genomic_DNA.
DR PIR; S56403; S56403.
DR RefSeq; NP_418599.1; NC_000913.3.
DR RefSeq; WP_001177639.1; NZ_SSZK01000052.1.
DR AlphaFoldDB; P0AF63; -.
DR SMR; P0AF63; -.
DR BioGRID; 4262026; 16.
DR DIP; DIP-48211N; -.
DR IntAct; P0AF63; 1.
DR STRING; 511145.b4178; -.
DR jPOST; P0AF63; -.
DR PaxDb; P0AF63; -.
DR PRIDE; P0AF63; -.
DR DNASU; 948693; -.
DR EnsemblBacteria; AAC77135; AAC77135; b4178.
DR EnsemblBacteria; BAE78179; BAE78179; BAE78179.
DR GeneID; 67414796; -.
DR GeneID; 948693; -.
DR KEGG; ecj:JW4136; -.
DR KEGG; eco:b4178; -.
DR PATRIC; fig|1411691.4.peg.2523; -.
DR EchoBASE; EB1197; -.
DR eggNOG; COG1959; Bacteria.
DR HOGENOM; CLU_107144_2_1_6; -.
DR InParanoid; P0AF63; -.
DR OMA; AQEAFYA; -.
DR PhylomeDB; P0AF63; -.
DR BioCyc; EcoCyc:EG11212-MON; -.
DR PRO; PR:P0AF63; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:EcoCyc.
DR Gene3D; 1.10.10.10; -; 1.
DR HAMAP; MF_01177; HTH_type_NsrR; 1.
DR InterPro; IPR030489; TR_Rrf2-type_CS.
DR InterPro; IPR000944; Tscrpt_reg_Rrf2.
DR InterPro; IPR023761; Tscrpt_rep_HTH_NsrR.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR33221; PTHR33221; 1.
DR Pfam; PF02082; Rrf2; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR TIGRFAMs; TIGR00738; rrf2_super; 1.
DR PROSITE; PS01332; HTH_RRF2_1; 1.
DR PROSITE; PS51197; HTH_RRF2_2; 1.
PE 3: Inferred from homology;
KW 2Fe-2S; DNA-binding; Iron; Iron-sulfur; Metal-binding; Reference proteome;
KW Repressor; Transcription; Transcription regulation.
FT CHAIN 1..141
FT /note="HTH-type transcriptional repressor NsrR"
FT /id="PRO_0000109555"
FT DOMAIN 2..129
FT /note="HTH rrf2-type"
FT DNA_BIND 28..51
FT /note="H-T-H motif"
FT /evidence="ECO:0000250"
FT BINDING 91
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000255"
FT BINDING 96
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000255"
FT BINDING 102
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000255"
SQ SEQUENCE 141 AA; 15593 MW; 6A783840023134D7 CRC64;
MQLTSFTDYG LRALIYMASL PEGRMTSISE VTDVYGVSRN HMVKIINQLS RAGYVTAVRG
KNGGIRLGKP ASAIRIGDVV RELEPLSLVN CSSEFCHITP ACRLKQALSK AVQSFLTELD
NYTLADLVEE NQPLYKLLLV E
