NSRR_SHIFL
ID NSRR_SHIFL Reviewed; 141 AA.
AC P0AF66; P21498; P72442;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=HTH-type transcriptional repressor NsrR {ECO:0000255|HAMAP-Rule:MF_01177};
GN Name=nsrR {ECO:0000255|HAMAP-Rule:MF_01177};
GN OrderedLocusNames=SF4333, S4601;
OS Shigella flexneri.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=623;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=YSH6000 / Serotype 2a;
RX PubMed=1400189; DOI=10.1128/jb.174.20.6359-6367.1992;
RA Tobe T., Sasakawa C., Okada N., Honma Y., Yoshikawa M.;
RT "vacB, a novel chromosomal gene required for expression of virulence genes
RT on the large plasmid of Shigella flexneri.";
RL J. Bacteriol. 174:6359-6367(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=301 / Serotype 2a;
RX PubMed=12384590; DOI=10.1093/nar/gkf566;
RA Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT through comparison with genomes of Escherichia coli K12 and O157.";
RL Nucleic Acids Res. 30:4432-4441(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT "Complete genome sequence and comparative genomics of Shigella flexneri
RT serotype 2a strain 2457T.";
RL Infect. Immun. 71:2775-2786(2003).
CC -!- FUNCTION: Nitric oxide-sensitive repressor of genes involved in
CC protecting the cell against nitrosative stress. May require iron for
CC activity. {ECO:0000255|HAMAP-Rule:MF_01177}.
CC -!- COFACTOR:
CC Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01177};
CC Note=Binds 1 [2Fe-2S] cluster per subunit. {ECO:0000255|HAMAP-
CC Rule:MF_01177};
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DR EMBL; D11024; BAA01776.1; -; Genomic_DNA.
DR EMBL; AE005674; AAN45750.1; -; Genomic_DNA.
DR EMBL; AE014073; AAP19533.1; -; Genomic_DNA.
DR RefSeq; NP_710043.1; NC_004337.2.
DR RefSeq; WP_001177639.1; NZ_UIQL01000058.1.
DR AlphaFoldDB; P0AF66; -.
DR SMR; P0AF66; -.
DR STRING; 198214.SF4333; -.
DR EnsemblBacteria; AAN45750; AAN45750; SF4333.
DR EnsemblBacteria; AAP19533; AAP19533; S4601.
DR GeneID; 1024386; -.
DR GeneID; 67414796; -.
DR KEGG; sfl:SF4333; -.
DR KEGG; sfx:S4601; -.
DR PATRIC; fig|198214.7.peg.5107; -.
DR HOGENOM; CLU_107144_2_1_6; -.
DR OMA; AQEAFYA; -.
DR OrthoDB; 1765806at2; -.
DR Proteomes; UP000001006; Chromosome.
DR Proteomes; UP000002673; Chromosome.
DR GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEA:InterPro.
DR Gene3D; 1.10.10.10; -; 1.
DR HAMAP; MF_01177; HTH_type_NsrR; 1.
DR InterPro; IPR030489; TR_Rrf2-type_CS.
DR InterPro; IPR000944; Tscrpt_reg_Rrf2.
DR InterPro; IPR023761; Tscrpt_rep_HTH_NsrR.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR33221; PTHR33221; 1.
DR Pfam; PF02082; Rrf2; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR TIGRFAMs; TIGR00738; rrf2_super; 1.
DR PROSITE; PS01332; HTH_RRF2_1; 1.
DR PROSITE; PS51197; HTH_RRF2_2; 1.
PE 3: Inferred from homology;
KW 2Fe-2S; DNA-binding; Iron; Iron-sulfur; Metal-binding; Reference proteome;
KW Repressor; Transcription; Transcription regulation.
FT CHAIN 1..141
FT /note="HTH-type transcriptional repressor NsrR"
FT /id="PRO_0000109558"
FT DOMAIN 2..129
FT /note="HTH rrf2-type"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01177"
FT DNA_BIND 28..51
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01177"
FT BINDING 91
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01177"
FT BINDING 96
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01177"
FT BINDING 102
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01177"
SQ SEQUENCE 141 AA; 15593 MW; 6A783840023134D7 CRC64;
MQLTSFTDYG LRALIYMASL PEGRMTSISE VTDVYGVSRN HMVKIINQLS RAGYVTAVRG
KNGGIRLGKP ASAIRIGDVV RELEPLSLVN CSSEFCHITP ACRLKQALSK AVQSFLTELD
NYTLADLVEE NQPLYKLLLV E
