NSRR_STRCO
ID NSRR_STRCO Reviewed; 148 AA.
AC Q9L132;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=HTH-type transcriptional repressor NsrR;
GN Name=nsrR; OrderedLocusNames=SCO7427; ORFNames=SC6D11.23;
OS Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145).
OC Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC Streptomyces; Streptomyces albidoflavus group.
OX NCBI_TaxID=100226;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-471 / A3(2) / M145;
RX PubMed=12000953; DOI=10.1038/417141a;
RA Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L.,
RA Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D.,
RA Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A.,
RA Fraser A., Goble A., Hidalgo J., Hornsby T., Howarth S., Huang C.-H.,
RA Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E.,
RA Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D.,
RA Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A.,
RA Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A.;
RT "Complete genome sequence of the model actinomycete Streptomyces coelicolor
RT A3(2).";
RL Nature 417:141-147(2002).
RN [2]
RP DNA-BINDING, COFACTOR, AND SUBUNIT.
RC STRAIN=ATCC BAA-471 / A3(2) / M145;
RX PubMed=18989365; DOI=10.1371/journal.pone.0003623;
RA Tucker N.P., Hicks M.G., Clarke T.A., Crack J.C., Chandra G., Le Brun N.E.,
RA Dixon R., Hutchings M.I.;
RT "The transcriptional repressor protein NsrR senses nitric oxide directly
RT via a [2Fe-2S] cluster.";
RL PLoS ONE 3:E3623-E3623(2008).
CC -!- FUNCTION: Binds DNA; this binding is disrupted by nitrosylation upon
CC exposure to nitric oxide (NO) and also by EDTA and iron chelators. The
CC 2Fe-2S cluster is stable in the presence of O(2).
CC -!- COFACTOR:
CC Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135;
CC Evidence={ECO:0000269|PubMed:18989365};
CC Note=Binds 1 [2Fe-2S] cluster per subunit.
CC {ECO:0000269|PubMed:18989365};
CC -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:18989365}.
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DR EMBL; AL939131; CAB76346.1; -; Genomic_DNA.
DR RefSeq; NP_631476.1; NC_003888.3.
DR RefSeq; WP_011031657.1; NZ_VNID01000005.1.
DR PDB; 5N07; X-ray; 1.95 A; A=1-148.
DR PDB; 5N08; X-ray; 3.90 A; A/B/C/D/E=1-148.
DR PDBsum; 5N07; -.
DR PDBsum; 5N08; -.
DR AlphaFoldDB; Q9L132; -.
DR SMR; Q9L132; -.
DR STRING; 100226.SCO7427; -.
DR GeneID; 1102865; -.
DR KEGG; sco:SCO7427; -.
DR PATRIC; fig|100226.15.peg.7537; -.
DR eggNOG; COG1959; Bacteria.
DR HOGENOM; CLU_107144_2_0_11; -.
DR InParanoid; Q9L132; -.
DR OMA; AQEAFYA; -.
DR PhylomeDB; Q9L132; -.
DR Proteomes; UP000001973; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR000944; Tscrpt_reg_Rrf2.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR33221; PTHR33221; 1.
DR Pfam; PF02082; Rrf2; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR TIGRFAMs; TIGR00738; rrf2_super; 1.
DR PROSITE; PS51197; HTH_RRF2_2; 1.
PE 1: Evidence at protein level;
KW 2Fe-2S; 3D-structure; DNA-binding; Iron; Iron-sulfur; Metal-binding;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..148
FT /note="HTH-type transcriptional repressor NsrR"
FT /id="PRO_0000368278"
FT DOMAIN 2..132
FT /note="HTH rrf2-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00540"
FT DNA_BIND 29..52
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00540"
FT BINDING 93
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000305"
FT BINDING 99
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000305"
FT BINDING 105
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000305"
FT HELIX 5..18
FT /evidence="ECO:0007829|PDB:5N07"
FT HELIX 29..35
FT /evidence="ECO:0007829|PDB:5N07"
FT HELIX 40..52
FT /evidence="ECO:0007829|PDB:5N07"
FT STRAND 55..57
FT /evidence="ECO:0007829|PDB:5N07"
FT STRAND 66..68
FT /evidence="ECO:0007829|PDB:5N07"
FT HELIX 70..74
FT /evidence="ECO:0007829|PDB:5N07"
FT HELIX 77..85
FT /evidence="ECO:0007829|PDB:5N07"
FT STRAND 93..97
FT /evidence="ECO:0007829|PDB:5N07"
FT TURN 100..103
FT /evidence="ECO:0007829|PDB:5N07"
FT HELIX 106..122
FT /evidence="ECO:0007829|PDB:5N07"
FT HELIX 127..131
FT /evidence="ECO:0007829|PDB:5N07"
FT HELIX 135..141
FT /evidence="ECO:0007829|PDB:5N07"
SQ SEQUENCE 148 AA; 15954 MW; 75FD46017BF13729 CRC64;
MRLTKFTDLA LRSLMRLAVV RDGDEPLATR EVAEVVGVPY THAAKAITRL QHLGVVEARR
GRGGGLTLTD LGRRVSVGWL VRELEGEAEV VDCEGDNPCP LRGACRLRRA LRDAQEAFYA
ALDPLTVTDL VAAPTGPVLL GLTDRPSG
