ID   NSS_BUNGE               Reviewed;         109 AA.
AC   P16992;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   02-DEC-2020, entry version 48.
DE   RecName: Full=Non-structural protein NS-S;
GN   Name=N;
OS   Bunyavirus germiston.
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC   Ellioviricetes; Bunyavirales; Peribunyaviridae; Orthobunyavirus.
OX   NCBI_TaxID=11574;
OH   NCBI_TaxID=53527; Culex.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=3660942; DOI=10.1016/0168-1702(87)90035-9;
RA   Gerbaud S., Vialat P., Pardigon N., Wychowski C., Girard M., Bouloy M.;
RT   "The S segment of the Germiston virus RNA genome can code for three
RT   proteins.";
RL   Virus Res. 8:1-13(1987).
CC   -!- FUNCTION: Inhibits host transcriptional machinery, by producing
CC       modifications to the phosphorylation state of the C-terminal domain
CC       (CTD) of RNA polymerase II. Inhibits phosphorylation at serine 2 in the
CC       heptapeptide repeat (YSPTSPS) of the CTD of RNA polymerase II,
CC       suggesting that the elongation step of transcription and/or 3'-end
CC       processing is prevented. Inhibition of host transcription machinery
CC       leads to shut off of host cell protein synthesis and inhibition of the
CC       host innate immune response. NSs also seems to be involved in the
CC       nuclear relocalization of host PABP1 (By similarity). {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative initiation; Named isoforms=2;
CC       Name=NSS;
CC         IsoId=P16992-1; Sequence=Displayed;
CC       Name=N;
CC         IsoId=P16993-1; Sequence=External;
CC   -!- MISCELLANEOUS: [Isoform NSS]: Produced by alternative initiation in the
CC       N gene, but encoded on another frame.
CC   -!- SIMILARITY: Belongs to the orthobunyavirus NS-S protein family.
CC       {ECO:0000305}.
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DR   EMBL; M19420; AAA87604.1; -; Genomic_RNA.
DR   GO; GO:0039657; P:suppression by virus of host gene expression; IEA:UniProtKB-KW.
DR   InterPro; IPR000797; Bunya_NSs.
DR   Pfam; PF01104; Bunya_NS-S; 1.
DR   PIRSF; PIRSF003954; NS-S_OrthobunV; 1.
PE   3: Inferred from homology;
KW   Alternative initiation; Eukaryotic host gene expression shutoff by virus;
KW   Eukaryotic host transcription shutoff by virus;
KW   Host gene expression shutoff by virus; Host-virus interaction;
KW   Inhibition of eukaryotic host transcription initiation by virus.
FT   CHAIN           1..109
FT                   /note="Non-structural protein NS-S"
FT                   /id="PRO_0000221972"
SQ   SEQUENCE   109 AA;  11891 MW;  1A49188EC6818BC0 CRC64;
     MSLITSGVLL TQSQDTLTFS VTTCQGLRLT KFASSTLKDA RLKIVSQKEV NGKLRLTLGA
     GRYLYSIRIS LETGTMQCLT TVLPSTVSVD TLPGTYLEST LQRQNQKSS