ID   NSS_BUNL7               Reviewed;          47 AA.
AC   P09614;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   02-DEC-2020, entry version 51.
DE   RecName: Full=Non-structural protein NS-S;
DE   Flags: Fragment;
GN   Name=N;
OS   Bunyavirus La Crosse (isolate Aedes triseriatus/United States/L74/1974).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC   Ellioviricetes; Bunyavirales; Peribunyaviridae; Orthobunyavirus;
OC   La Crosse orthobunyavirus.
OX   NCBI_TaxID=11578;
OH   NCBI_TaxID=9850; Cervidae (deer).
OH   NCBI_TaxID=9606; Homo sapiens (Human).
OH   NCBI_TaxID=7162; Ochlerotatus triseriatus (Eastern treehole mosquito) (Aedes triseriatus).
OH   NCBI_TaxID=13712; Tamias.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=7086954; DOI=10.1128/jvi.41.1.119-128.1982;
RA   Clerx-Van Haaster C.M., Akashi H., Auperin D.D., Bishop D.H.L.;
RT   "Nucleotide sequence analyses and predicted coding of bunyavirus genome RNA
RT   species.";
RL   J. Virol. 41:119-128(1982).
CC   -!- FUNCTION: Inhibits host transcriptional machinery, by producing
CC       modifications to the phosphorylation state of the C-terminal domain
CC       (CTD) of RNA polymerase II. Inhibits phosphorylation at serine 2 in the
CC       heptapeptide repeat (YSPTSPS) of the CTD of RNA polymerase II,
CC       suggesting that the elongation step of transcription and/or 3'-end
CC       processing is prevented. Inhibition of host transcription machinery
CC       leads to shut off of host cell protein synthesis and inhibition of the
CC       host innate immune response. NSs also seems to be involved in the
CC       nuclear relocalization of host PABP1 (By similarity). {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative initiation; Named isoforms=2;
CC       Name=NSS;
CC         IsoId=P09614-1; Sequence=Displayed;
CC       Name=N;
CC         IsoId=P09614-2; Sequence=Not described;
CC   -!- MISCELLANEOUS: [Isoform NSS]: Produced by alternative initiation in the
CC       N gene, but encoded on another frame.
CC   -!- SIMILARITY: Belongs to the orthobunyavirus NS-S protein family.
CC       {ECO:0000305}.
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DR   EMBL; J02232; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   GO; GO:0039657; P:suppression by virus of host gene expression; IEA:UniProtKB-KW.
DR   InterPro; IPR000797; Bunya_NSs.
DR   Pfam; PF01104; Bunya_NS-S; 1.
DR   PIRSF; PIRSF003954; NS-S_OrthobunV; 1.
PE   3: Inferred from homology;
KW   Alternative initiation; Eukaryotic host gene expression shutoff by virus;
KW   Eukaryotic host transcription shutoff by virus;
KW   Host gene expression shutoff by virus; Host-virus interaction;
KW   Inhibition of eukaryotic host transcription initiation by virus.
FT   CHAIN           1..>47
FT                   /note="Non-structural protein NS-S"
FT                   /id="PRO_0000221974"
FT   NON_TER         47
SQ   SEQUENCE   47 AA;  5364 MW;  027351A518944E9F CRC64;
     MMSHQQVQIN LILMQGIWTS VLKMQNHSTL LQLGSSSTMP QRPRLLS