ID   NSS_BUNLC               Reviewed;          92 AA.
AC   P04874;
DT   13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT   13-AUG-1987, sequence version 1.
DT   02-DEC-2020, entry version 59.
DE   RecName: Full=Non-structural protein NS-S;
GN   Name=N;
OS   Bunyavirus La Crosse.
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC   Ellioviricetes; Bunyavirales; Peribunyaviridae; Orthobunyavirus;
OC   La Crosse orthobunyavirus.
OX   NCBI_TaxID=11577;
OH   NCBI_TaxID=9850; Cervidae (deer).
OH   NCBI_TaxID=9606; Homo sapiens (Human).
OH   NCBI_TaxID=7162; Ochlerotatus triseriatus (Eastern treehole mosquito) (Aedes triseriatus).
OH   NCBI_TaxID=13712; Tamias.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=6834480; DOI=10.1128/jvi.45.3.1155-1158.1983;
RA   Akashi H., Bishop D.H.L.;
RT   "Comparison of the sequences and coding of La Crosse and snowshoe hare
RT   bunyavirus S RNA species.";
RL   J. Virol. 45:1155-1158(1983).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=6684362; DOI=10.1016/0042-6822(83)90271-4;
RA   Cabradilla C.D. Jr., Holloway B.P., Obijeski J.F.;
RT   "Molecular cloning and sequencing of the La Crosse virus S RNA.";
RL   Virology 128:463-468(1983).
CC   -!- FUNCTION: Inhibits host transcriptional machinery, by producing
CC       modifications to the phosphorylation state of the C-terminal domain
CC       (CTD) of RNA polymerase II. Inhibits phosphorylation at serine 2 in the
CC       heptapeptide repeat (YSPTSPS) of the CTD of RNA polymerase II,
CC       suggesting that the elongation step of transcription and/or 3'-end
CC       processing is prevented. Inhibition of host transcription machinery
CC       leads to shut off of host cell protein synthesis and inhibition of the
CC       host innate immune response. NSs also seems to be involved in the
CC       nuclear relocalization of host PABP1 (By similarity). {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative initiation; Named isoforms=2;
CC       Name=NSS;
CC         IsoId=P04874-1; Sequence=Displayed;
CC       Name=N;
CC         IsoId=P04873-1; Sequence=External;
CC   -!- MISCELLANEOUS: [Isoform NSS]: Produced by alternative initiation in the
CC       N gene, but encoded on another frame.
CC   -!- SIMILARITY: Belongs to the orthobunyavirus NS-S protein family.
CC       {ECO:0000305}.
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DR   EMBL; K00610; AAA42783.1; -; Genomic_RNA.
DR   EMBL; K00108; AAA42780.1; -; Genomic_RNA.
DR   PIR; A04106; MNVULV.
DR   Proteomes; UP000232774; Genome.
DR   GO; GO:0001934; P:positive regulation of protein phosphorylation; IDA:CACAO.
DR   GO; GO:0039657; P:suppression by virus of host gene expression; IEA:UniProtKB-KW.
DR   InterPro; IPR000797; Bunya_NSs.
DR   Pfam; PF01104; Bunya_NS-S; 1.
DR   PIRSF; PIRSF003954; NS-S_OrthobunV; 1.
PE   3: Inferred from homology;
KW   Alternative initiation; Eukaryotic host gene expression shutoff by virus;
KW   Eukaryotic host transcription shutoff by virus;
KW   Host gene expression shutoff by virus; Host-virus interaction;
KW   Inhibition of eukaryotic host transcription initiation by virus;
KW   Reference proteome.
FT   CHAIN           1..92
FT                   /note="Non-structural protein NS-S"
FT                   /id="PRO_0000221973"
FT   CONFLICT        40
FT                   /note="P -> L (in Ref. 2; AAA42780)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   92 AA;  10441 MW;  03572181F26C6348 CRC64;
     MMSHQQVQMD LILMQGIWTS VLKMQNHSTL LQLGSSSSMP QRPRLLSRVS QRGRLTLNLE
     SGRWRLSIII FLETGTTQLV TTILPSTDYL GI