ID   NSS_BUNYW               Reviewed;         101 AA.
AC   P16494;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   23-FEB-2022, entry version 69.
DE   RecName: Full=Non-structural protein NS-S;
GN   Name=N;
OS   Bunyamwera virus (BUNV).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC   Ellioviricetes; Bunyavirales; Peribunyaviridae; Orthobunyavirus.
OX   NCBI_TaxID=35304;
OH   NCBI_TaxID=7159; Aedes aegypti (Yellowfever mosquito) (Culex aegypti).
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=2732714; DOI=10.1099/0022-1317-70-5-1281;
RA   Elliott R.M.;
RT   "Nucleotide sequence analysis of the small (S) RNA segment of Bunyamwera
RT   virus, the prototype of the family Bunyaviridae.";
RL   J. Gen. Virol. 70:1281-1285(1989).
RN   [2]
RP   FUNCTION.
RX   PubMed=15150262; DOI=10.1074/jbc.m400938200;
RA   Thomas D., Blakqori G., Wagner V., Banholzer M., Kessler N., Elliott R.M.,
RA   Haller O., Weber F.;
RT   "Inhibition of RNA polymerase II phosphorylation by a viral interferon
RT   antagonist.";
RL   J. Biol. Chem. 279:31471-31477(2004).
RN   [3]
RP   FUNCTION.
RX   PubMed=19193790; DOI=10.1128/jvi.02201-08;
RA   Blakqori G., van Knippenberg I., Elliott R.M.;
RT   "Bunyamwera orthobunyavirus S-segment untranslated regions mediate poly(A)
RT   tail-independent translation.";
RL   J. Virol. 83:3637-3646(2009).
CC   -!- FUNCTION: Inhibits host transcriptional machinery, by producing
CC       modifications to the phosphorylation state of the C-terminal domain
CC       (CTD) of RNA polymerase II. Inhibits phosphorylation at serine 2 in the
CC       heptapeptide repeat (YSPTSPS) of the CTD of RNA polymerase II,
CC       suggesting that the elongation step of transcription and/or 3'-end
CC       processing is prevented. Inhibition of host transcription machinery
CC       leads to shut off of host cell protein synthesis and inhibition of the
CC       host innate immune response. NSs also seems to be involved in the
CC       nuclear relocalization of host PABP1. {ECO:0000269|PubMed:15150262,
CC       ECO:0000269|PubMed:19193790}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative initiation; Named isoforms=2;
CC       Name=NSS;
CC         IsoId=P16494-1; Sequence=Displayed;
CC       Name=N;
CC         IsoId=P16495-1; Sequence=External;
CC   -!- MISCELLANEOUS: [Isoform NSS]: Produced by alternative initiation in the
CC       N gene, but encoded on another frame.
CC   -!- SIMILARITY: Belongs to the orthobunyavirus NS-S protein family.
CC       {ECO:0000305}.
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DR   EMBL; D00353; BAA00262.1; -; Genomic_RNA.
DR   PIR; B31365; MNVUBV.
DR   RefSeq; NP_047214.1; NC_001927.1. [P16494-1]
DR   GeneID; 2648218; -.
DR   KEGG; vg:2648218; -.
DR   Proteomes; UP000002476; Genome.
DR   GO; GO:0046725; P:negative regulation by virus of viral protein levels in host cell; IMP:CACAO.
DR   GO; GO:0039657; P:suppression by virus of host gene expression; IMP:CACAO.
DR   GO; GO:0039523; P:suppression by virus of host mRNA transcription via inhibition of RNA polymerase II activity; IMP:CACAO.
DR   GO; GO:0039501; P:suppression by virus of host type I interferon production; IDA:CACAO.
DR   GO; GO:0019080; P:viral gene expression; IMP:CACAO.
DR   InterPro; IPR000797; Bunya_NSs.
DR   Pfam; PF01104; Bunya_NS-S; 1.
DR   PIRSF; PIRSF003954; NS-S_OrthobunV; 1.
PE   3: Inferred from homology;
KW   Alternative initiation; Eukaryotic host gene expression shutoff by virus;
KW   Eukaryotic host transcription shutoff by virus;
KW   Host gene expression shutoff by virus; Host-virus interaction;
KW   Inhibition of host RNA polymerase II by virus; Reference proteome.
FT   CHAIN           1..101
FT                   /note="Non-structural protein NS-S"
FT                   /id="PRO_0000221976"
SQ   SEQUENCE   101 AA;  11024 MW;  823C9F75448C6368 CRC64;
     MMSLLTPAVL LTQRSHTLTL SVSTPLGLVM TTYESSTLKD ARLKLVSQKE VNGKLHLTLG
     AGRLLYIIRI FLATGTTQFL TMVLPSTASV DSLPGTYLRR C