ID   NSS_TULV                Reviewed;          90 AA.
AC   P0DTK1;
DT   25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT   25-MAY-2022, sequence version 1.
DT   03-AUG-2022, entry version 2.
DE   RecName: Full=Non-structural protein NS-S;
DE            Short=NSs;
GN   Name=N;
OS   Tula orthohantavirus (TULV) (Tula virus).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC   Ellioviricetes; Bunyavirales; Hantaviridae; Mammantavirinae;
OC   Orthohantavirus.
OX   NCBI_TaxID=1980494;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
OH   NCBI_TaxID=47230; Microtus arvalis (Common vole) (Field vole).
OH   NCBI_TaxID=523745; Microtus obscurus.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate Tula/Moravia/5302Ma/94;
RX   PubMed=8837887; DOI=10.1016/0168-1702(95)00086-0;
RA   Plyusnin A., Cheng Y., Vapalahti O., Pejcoch M., Unar J., Jelinkova Z.,
RA   Lehvaeslaiho H., Lundkvist A., Vaheri A.;
RT   "Genetic variation in Tula hantaviruses: sequence analysis of the S and M
RT   segments of strains from Central Europe.";
RL   Virus Res. 39:237-250(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate Tula/Moravia/5302v/95;
RX   PubMed=9000098; DOI=10.1099/0022-1317-77-12-3063;
RA   Vapalahti O., Lundkvist A., Kukkonen S.K., Cheng Y., Gilljam M.,
RA   Kanerva M., Manni T., Pejcoch M., Niemimaa J., Kaikusalo A., Henttonen H.,
RA   Vaheri A., Plyusnin A.;
RT   "Isolation and characterization of Tula virus, a distinct serotype in the
RT   genus Hantavirus, family Bunyaviridae.";
RL   J. Gen. Virol. 77:3063-3067(1996).
RN   [3]
RP   FUNCTION.
RX   PubMed=17705180; DOI=10.1002/jmv.20948;
RA   Jaaskelainen K.M., Kaukinen P., Minskaya E.S., Plyusnina A., Vapalahti O.,
RA   Elliott R.M., Weber F., Vaheri A., Plyusnin A.;
RT   "Tula and Puumala hantavirus NSs ORFs are functional and the products
RT   inhibit activation of the interferon-beta promoter.";
RL   J. Med. Virol. 79:1527-1536(2007).
RN   [4]
RP   CHARACTERIZATION.
RX   PubMed=18190677; DOI=10.1186/1743-422x-5-3;
RA   Jaeaeskelaeinen K.M., Plyusnina A., Lundkvist A., Vaheri A., Plyusnin A.;
RT   "Tula hantavirus isolate with the full-length ORF for nonstructural protein
RT   NSs survives for more consequent passages in interferon-competent cells
RT   than the isolate having truncated NSs ORF.";
RL   Virol. J. 5:3-3(2008).
RN   [5]
RP   SUBCELLULAR LOCATION.
RX   PubMed=19956987; DOI=10.1007/s00705-009-0546-y;
RA   Virtanen J.O., Jaeaeskelaeinen K.M., Djupsjoebacka J., Vaheri A.,
RA   Plyusnin A.;
RT   "Tula hantavirus NSs protein accumulates in the perinuclear area in
RT   infected and transfected cells.";
RL   Arch. Virol. 155:117-121(2010).
RN   [6]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=33478127; DOI=10.3390/v13010140;
RA   Gallo G., Caignard G., Badonnel K., Chevreux G., Terrier S., Szemiel A.,
RA   Roman-Sosa G., Binder F., Gu Q., Da Silva Filipe A., Ulrich R.G., Kohl A.,
RA   Vitour D., Tordo N., Ermonval M.;
RT   "Interactions of Viral Proteins from Pathogenic and Low or Non-Pathogenic
RT   Orthohantaviruses with Human Type I Interferon Signaling.";
RL   Viruses 13:0-0(2021).
CC   -!- FUNCTION: Antagonizes host type-I IFN signaling pathway.
CC       {ECO:0000269|PubMed:17705180, ECO:0000269|PubMed:33478127}.
CC   -!- SUBUNIT: Interacts with host MAVS; this interaction may reduce MAVS
CC       ubiquitination. {ECO:0000250|UniProtKB:Q80DP8}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm, host perinuclear region
CC       {ECO:0000269|PubMed:19956987}. Host cytoplasm
CC       {ECO:0000269|PubMed:33478127}. Host nucleus
CC       {ECO:0000269|PubMed:33478127}.
CC   -!- MISCELLANEOUS: Expressed from the S segment by a leaky scanning
CC       mechanism (By similarity). The sequence shown is that of isolate
CC       Tula/Moravia/5302Ma/94 (Probable). The isolate Tula/Moravia/5302Ma/94
CC       that contains the full length NSs survives for more consequent passages
CC       than the isolate Tula/Moravia/5302v/95, which contains a truncated NSs
CC       protein (PubMed:18190677). {ECO:0000250|UniProtKB:Q80DP8,
CC       ECO:0000269|PubMed:18190677, ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the hantavirus NS-S protein family.
CC       {ECO:0000305}.
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DR   EMBL; Z69991; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   EMBL; Z49915; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   Proteomes; UP000243699; Genome.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0044220; C:host cell perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0039503; P:suppression by virus of host innate immune response; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Host cytoplasm; Host nucleus; Host-virus interaction;
KW   Inhibition of host innate immune response by virus;
KW   Inhibition of host RLR pathway by virus; Viral immunoevasion.
FT   CHAIN           1..90
FT                   /note="Non-structural protein NS-S"
FT                   /id="PRO_0000455183"
FT   REGION          4..29
FT                   /note="Nucleolar signal"
FT                   /evidence="ECO:0000269|PubMed:33478127"
FT   VARIANT         1..23
FT                   /note="Missing (in strain:Tula/Moravia/5302v/95)"
SQ   SEQUENCE   90 AA;  10883 MW;  DF4B28D6A9AC8632 CRC64;
     MNSKLSLPGK NLKMQKRRWK PTRMMLTRAH YRVDGQLCQH WRTNWQTSRG SLQIWCQVKK
     WVKSLLTRLG LSRMITSRRD QAFDMEMSLM