ID   NST1_YEAS7              Reviewed;        1240 AA.
AC   A6ZRZ0;
DT   18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 1.
DT   03-AUG-2022, entry version 36.
DE   RecName: Full=Stress response protein NST1;
DE   AltName: Full=Negatively-affecting salt tolerance protein 1;
GN   Name=NST1; ORFNames=SCY_4701;
OS   Saccharomyces cerevisiae (strain YJM789) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=307796;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=YJM789;
RX   PubMed=17652520; DOI=10.1073/pnas.0701291104;
RA   Wei W., McCusker J.H., Hyman R.W., Jones T., Ning Y., Cao Z., Gu Z.,
RA   Bruno D., Miranda M., Nguyen M., Wilhelmy J., Komp C., Tamse R., Wang X.,
RA   Jia P., Luedi P., Oefner P.J., David L., Dietrich F.S., Li Y., Davis R.W.,
RA   Steinmetz L.M.;
RT   "Genome sequencing and comparative analysis of Saccharomyces cerevisiae
RT   strain YJM789.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:12825-12830(2007).
CC   -!- FUNCTION: With MSL1, acts as a negative regulator of salt tolerance.
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with MSL1. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the NST1 family. {ECO:0000305}.
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DR   EMBL; AAFW02000067; EDN62722.1; -; Genomic_DNA.
DR   AlphaFoldDB; A6ZRZ0; -.
DR   SMR; A6ZRZ0; -.
DR   PRIDE; A6ZRZ0; -.
DR   EnsemblFungi; EDN62722; EDN62722; SCY_4701.
DR   HOGENOM; CLU_267374_0_0_1; -.
DR   Proteomes; UP000007060; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   InterPro; IPR025279; NST1.
DR   Pfam; PF13945; NST1; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Cytoplasm; Phosphoprotein; Stress response.
FT   CHAIN           1..1240
FT                   /note="Stress response protein NST1"
FT                   /id="PRO_0000324463"
FT   REGION          1..76
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          446..593
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          634..744
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          953..980
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          998..1029
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1214..1240
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          616..777
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        1..17
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        456..483
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        484..514
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        524..559
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        560..587
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        953..969
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         266
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P53935"
SQ   SEQUENCE   1240 AA;  141404 MW;  ED1169104DCEA164 CRC64;
     MPPNSKSKRR KNKSKQHNKK NGNSDPEQSI NPTQLVPRME PELYHTESDY PTSRVIKRAP
     NGDVIVEPIN TDDDKKERTA NLTHNKDSMD SASSLAFTLD SHWESLSPEE KKTILRIEKE
     EVFNVIRNYQ DDHSCSCSVC GRRHLAMDQE MERIYNTLYA MDKDKDPETN PIKFHLGIIK
     ELQISKNQQQ NDLSSTKGEV VKNFLSSSTV GSLKEEVLHF KQKQLSKQEQ AHNETADNTS
     LLEENLNNIH INKTSSEISA NFNSVSDEEL QQKYSNFTKT FISSHPKIAE EYVQKMMMYP
     NIRALTDDLM NSNGQGFLNA IEDFVRDGQI QASKKDDSIT EDEASSTDLT DPKEFTTMLH
     SGKPLTEDEY ADLQRNIAER MTNAYDTASK KFKDVSQLEK ELFTRFMSGR DKKSFRELII
     QSFKNKFDGE LGPSVLAATL SSCFSSQSKD TSLDTDSIYE DEDEEDYDDY SEYAEDSEEV
     SEYEGIEAVE KPEHDEKSNG IRETLHLSYD HDHKRQNHPH HHYHSTSTHS EDELSEEEYI
     SDIELPHDPH KHFHRDDDIL DGDEDEPEEE DENEGDDEED TYDSGLDETD RLEEGRKLIQ
     IAITKLLQSR IMASYHEKQA DNNRLKLLQE LEEEKRKKRE KEEKKQKKRE KEKEKKRLQQ
     LAKEEEKRKR EEEKERLKKE LEEREMRRRE AQRKKVEEAK RKKDEERKRR LEEQQRREEM
     QEKQRKQKEE LKRKREEEKK RIREQKRLEQ EKLQKEKEEE ERQRLIAEDA LRKQKLNEEQ
     TSANILSAKP FTENGVGNPV SSQSHPNMTN YQEDNSCSIN DEILKMVNSV AASKPVSPTG
     FNVHDLLLPS TNNQMPAMEQ SHLSQPGNQN NHFGTTTIPN ALDLATKSSL QTENNYLMNS
     QTLENTSLLM HNNSSPTKLL PNDFGLSSWG GLTNTMSINP TCKPPVIQTS EMKSQAHKSS
     PQATMPSFGL PNGGTHRKSF TDELNTLTSM LSSSGFADTS LSSSGFPPSQ RSVWNDQKSS
     FSGPSTAGNF NNSSIQSGML LAPTLGSVES FPNRTSIWDS STTPMMNKSE LSGRNITSTA
     QDSPAFMASN IWSSNSQYNS PYLTSNVLQS PQISSGVDES HILDSIYNTY LAISPQDSLN
     PYIAIGTLFQ NLVGLNLDYS TFINKLISMQ GAYNCEFFTD NNGSITHVRF ARQTPAGHSK
     GLLNQLFSGL NDPTATPFTS RPHTSTGASF PIASSTTQTS