NSUN7_MOUSE
ID NSUN7_MOUSE Reviewed; 724 AA.
AC Q14AW5; Q8BS48; Q9D5S9;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 29-MAY-2007, sequence version 2.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=Putative methyltransferase NSUN7;
DE EC=2.1.1.-;
DE AltName: Full=NOL1/NOP2/Sun domain family member 7;
GN Name=Nsun7;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 77-724 (ISOFORM 4).
RC STRAIN=C57BL/6J; TISSUE=Embryo, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=17442852; DOI=10.1095/biolreprod.106.058669;
RA Harris T., Marquez B., Suarez S., Schimenti J.;
RT "Sperm motility defects and infertility in male mice with a mutation in
RT Nsun7, a member of the Sun domain-containing family of putative RNA
RT methyltransferases.";
RL Biol. Reprod. 77:376-382(2007).
CC -!- FUNCTION: May have S-adenosyl-L-methionine-dependent methyl-transferase
CC activity. {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=Q14AW5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q14AW5-2; Sequence=VSP_025984;
CC Name=3;
CC IsoId=Q14AW5-3; Sequence=VSP_025982, VSP_025983;
CC Name=4;
CC IsoId=Q14AW5-4; Sequence=VSP_025985;
CC -!- TISSUE SPECIFICITY: Expressed in testis. {ECO:0000269|PubMed:17442852}.
CC -!- DEVELOPMENTAL STAGE: Low expression in 12-day-old testes when the
CC majority of advanced cells are in zygonema, but increased by 14 to 18
CC days of age when most spermatocytes are in pachynema.
CC {ECO:0000269|PubMed:17442852}.
CC -!- DISRUPTION PHENOTYPE: Male infertility. A mutation which results in the
CC conversion of codon 333, encoding glutamine, into a premature stop
CC codon is detected in chemically-induced mutant Ste5Jcs1 mice; these
CC mice are characterized by sperm motility defects and infertility.
CC {ECO:0000269|PubMed:17442852}.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. RsmB/NOP family. {ECO:0000255|PROSITE-ProRule:PRU01023}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB29644.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK014968; BAB29644.1; ALT_INIT; mRNA.
DR EMBL; AK035181; BAC28971.1; -; mRNA.
DR EMBL; BC115878; AAI15879.1; -; mRNA.
DR EMBL; BC116658; AAI16659.1; -; mRNA.
DR CCDS; CCDS80291.1; -. [Q14AW5-3]
DR RefSeq; NP_001289762.1; NM_001302833.1.
DR RefSeq; NP_001289763.1; NM_001302834.1. [Q14AW5-3]
DR RefSeq; NP_081878.1; NM_027602.2.
DR AlphaFoldDB; Q14AW5; -.
DR SMR; Q14AW5; -.
DR IntAct; Q14AW5; 2.
DR STRING; 10090.ENSMUSP00000109349; -.
DR PhosphoSitePlus; Q14AW5; -.
DR PaxDb; Q14AW5; -.
DR PRIDE; Q14AW5; -.
DR ProteomicsDB; 287827; -. [Q14AW5-1]
DR ProteomicsDB; 287828; -. [Q14AW5-2]
DR ProteomicsDB; 287829; -. [Q14AW5-3]
DR ProteomicsDB; 287830; -. [Q14AW5-4]
DR Antibodypedia; 11775; 40 antibodies from 12 providers.
DR DNASU; 70918; -.
DR Ensembl; ENSMUST00000202994; ENSMUSP00000144498; ENSMUSG00000029206. [Q14AW5-3]
DR GeneID; 70918; -.
DR KEGG; mmu:70918; -.
DR UCSC; uc008xos.2; mouse. [Q14AW5-3]
DR CTD; 79730; -.
DR MGI; MGI:1918168; Nsun7.
DR VEuPathDB; HostDB:ENSMUSG00000029206; -.
DR eggNOG; KOG2360; Eukaryota.
DR GeneTree; ENSGT00940000157352; -.
DR InParanoid; Q14AW5; -.
DR OrthoDB; 1128973at2759; -.
DR PhylomeDB; Q14AW5; -.
DR BioGRID-ORCS; 70918; 1 hit in 71 CRISPR screens.
DR ChiTaRS; Nsun7; mouse.
DR PRO; PR:Q14AW5; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q14AW5; protein.
DR Bgee; ENSMUSG00000029206; Expressed in ventricular system choroidal fissure and 78 other tissues.
DR ExpressionAtlas; Q14AW5; baseline and differential.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0030317; P:flagellated sperm motility; IMP:MGI.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0030382; P:sperm mitochondrion organization; IMP:MGI.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR001678; MeTrfase_RsmB/NOP2.
DR InterPro; IPR042620; NSUN7.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR14663; PTHR14663; 1.
DR Pfam; PF01189; Methyltr_RsmB-F; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
DR PROSITE; PS51686; SAM_MT_RSMB_NOP; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Methyltransferase; Reference proteome; RNA-binding;
KW S-adenosyl-L-methionine; Transferase.
FT CHAIN 1..724
FT /note="Putative methyltransferase NSUN7"
FT /id="PRO_0000289240"
FT REGION 542..574
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 595..629
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 698..724
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 558..572
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 595..628
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 700..716
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 444
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01023"
FT VAR_SEQ 36
FT /note="N -> NS (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_025982"
FT VAR_SEQ 399..433
FT /note="DKSLLQDLSQGGLPKDKLETLVQQQFEQLTHAMKF -> V (in isoform
FT 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_025983"
FT VAR_SEQ 691..724
FT /note="VGVPRYLTSSTSRRKEKVKESTTSSHVRHPRPWL -> DSSREDRINNFLIK
FT (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_025984"
FT VAR_SEQ 691..724
FT /note="VGVPRYLTSSTSRRKEKVKESTTSSHVRHPRPWL -> RFCSSLGLF (in
FT isoform 4)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_025985"
SQ SEQUENCE 724 AA; 81968 MW; 55AEF475776D8455 CRC64;
MLDPTSERDL FDQENMEEIS QLASLEMSGD VVANTNSTVV LEKPSYPDSV YVTAANIFQG
IRIQRSPDKV IINYGSEPLQ PSSSRSEDES FQRLSYELAF STLKYQDILE SILIDSYIFS
STTIASQLNS LIIVMLYDFQ DRKFQPRILS ENEETIPEVQ EVENLLNGFK TKLAAALARC
RIKHDALSIY HILPETVRKQ EQRASTLPLY AWINTSKISL EEVYNNLRRK GYSKVKSITS
VNEKVYAVDQ HCFNVLIFPA HLKTDLLNID LIKDYKLIFQ DKSRSLAVHS VKALINIDDD
VLMVNTGSWY TVAHMSILTS GHTSKIFVCG IQQEEKDFNA RKLFTRMGCQ NIEILHETFL
SIESKDHRLQ NVKVILLLPR CSSLGVSNPV EFILNEHEDK SLLQDLSQGG LPKDKLETLV
QQQFEQLTHA MKFTKVQAIV YCTCSVSKEE NEDVVEKALE YQSSGVKMQP YRLSPPVLPL
CTLKEIELSM DRFFRLEPSD MNNGCFLSIL TRERDPSETV SVKDVLARAA AKGLLEGVEV
GKTLKRDKKR KKSKALPSRA PHHGDPLRDH LAVDGNDTSN VQMKISELLH RESKISTSTK
MSAPAKTVSQ AGTSSQVRKP SKPLSTPLVR NFSRPVERPT NFVRARPEGK VIPLKPIEIV
LPPVIFPLSS QGPRVQMPAT HFYYRFIGSK VGVPRYLTSS TSRRKEKVKE STTSSHVRHP
RPWL
