NSY7_CAEEL
ID NSY7_CAEEL Reviewed; 294 AA.
AC H2KYW5; C1P623;
DT 07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT 21-MAR-2012, sequence version 1.
DT 03-AUG-2022, entry version 59.
DE RecName: Full=Transcription factor nsy-7 {ECO:0000303|PubMed:19204119};
DE AltName: Full=Neuronal symmetry protein 7 {ECO:0000312|WormBase:C18F3.4a};
GN Name=nsy-7 {ECO:0000312|WormBase:C18F3.4a};
GN ORFNames=C18F3.4 {ECO:0000312|WormBase:C18F3.4a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP DISRUPTION PHENOTYPE, DNA-BINDING, AND MUTAGENESIS OF HIS-179.
RX PubMed=19204119; DOI=10.1101/gad.1763509;
RA Lesch B.J., Gehrke A.R., Bulyk M.L., Bargmann C.I.;
RT "Transcriptional regulation and stabilization of left-right neuronal
RT identity in C. elegans.";
RL Genes Dev. 23:345-358(2009).
RN [3] {ECO:0000305}
RP FUNCTION.
RX PubMed=20713521; DOI=10.1101/gad.1932610;
RA Lesch B.J., Bargmann C.I.;
RT "The homeodomain protein hmbx-1 maintains asymmetric gene expression in
RT adult C. elegans olfactory neurons.";
RL Genes Dev. 24:1802-1815(2010).
RN [4] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=31767767; DOI=10.1073/pnas.1908168116;
RA Alqadah A., Hsieh Y.W., Xiong R., Lesch B.J., Chang C., Chuang C.F.;
RT "A universal transportin protein drives stochastic choice of olfactory
RT neurons via specific nuclear import of a sox-2-activating factor.";
RL Proc. Natl. Acad. Sci. U.S.A. 116:25137-25146(2019).
CC -!- FUNCTION: Transcriptional regulator which binds DNA consensus sequence
CC 5'-CCTTAAC-3' (PubMed:19204119, PubMed:31767767). Plays a role in
CC establishing and maintaining asymmetric cell fates in chemosensory AWC
CC neurons during larval neuronal development (PubMed:19204119,
CC PubMed:20713521, PubMed:31767767). This is achieved by repressing the
CC expression of multiple AWC (OFF) genes, including srsx-3 and hlh-11 in
CC the AWC (ON) neuron (PubMed:19204119). Activates expression of sox-2 in
CC the AWC (ON) neuron (PubMed:31767767). {ECO:0000269|PubMed:19204119,
CC ECO:0000269|PubMed:20713521, ECO:0000269|PubMed:31767767}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305|PubMed:19204119,
CC ECO:0000305|PubMed:31767767}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=a {ECO:0000312|WormBase:C18F3.4a};
CC IsoId=H2KYW5-1; Sequence=Displayed;
CC Name=b {ECO:0000312|WormBase:C18F3.4b};
CC IsoId=H2KYW5-2; Sequence=VSP_060757;
CC -!- TISSUE SPECIFICITY: Expressed widely, including gut, the amphid sheath
CC glial cells, and head and tail neurons including AWC, ASE, and ASH
CC (PubMed:19204119). Expressed in AWC (ON) olfactory neuron but not AWC
CC (OFF) (PubMed:19204119, PubMed:31767767). {ECO:0000269|PubMed:19204119,
CC ECO:0000269|PubMed:31767767}.
CC -!- DEVELOPMENTAL STAGE: At the threefold embryonic stage, expressed
CC asymmetrically in one of the two AWC neurons (PubMed:19204119). A
CC subset of embryos show bilateral expression in AWC neurons, but this
CC declines after larval stage L1 (PubMed:19204119).
CC {ECO:0000269|PubMed:19204119}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown leads to misexpression of
CC markers of the asymmetric state of AWC neurons.
CC {ECO:0000269|PubMed:19204119}.
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DR EMBL; BX284604; CCD65188.1; -; Genomic_DNA.
DR EMBL; BX284604; CCD65189.2; -; Genomic_DNA.
DR RefSeq; NP_001255309.1; NM_001268380.1. [H2KYW5-1]
DR RefSeq; NP_001255310.2; NM_001268381.2. [H2KYW5-2]
DR AlphaFoldDB; H2KYW5; -.
DR SMR; H2KYW5; -.
DR IntAct; H2KYW5; 3.
DR STRING; 6239.C18F3.4a; -.
DR PaxDb; H2KYW5; -.
DR EnsemblMetazoa; C18F3.4a.1; C18F3.4a.1; WBGene00044508. [H2KYW5-1]
DR EnsemblMetazoa; C18F3.4b.1; C18F3.4b.1; WBGene00044508. [H2KYW5-2]
DR GeneID; 3896760; -.
DR KEGG; cel:CELE_C18F3.4; -.
DR CTD; 3896760; -.
DR WormBase; C18F3.4a; CE42065; WBGene00044508; nsy-7.
DR WormBase; C18F3.4b; CE49558; WBGene00044508; nsy-7.
DR eggNOG; ENOG502TG34; Eukaryota.
DR HOGENOM; CLU_947422_0_0_1; -.
DR InParanoid; H2KYW5; -.
DR OMA; QFVSNHS; -.
DR OrthoDB; 1366195at2759; -.
DR SignaLink; H2KYW5; -.
DR Proteomes; UP000001940; Chromosome IV.
DR Bgee; WBGene00044508; Expressed in embryo and 3 other tissues.
DR ExpressionAtlas; H2KYW5; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:WormBase.
DR GO; GO:0035545; P:determination of left/right asymmetry in nervous system; IMP:WormBase.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:WormBase.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR001356; Homeobox_dom.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; DNA-binding; Nucleus; Reference proteome;
KW Repressor; Transcription; Transcription regulation.
FT CHAIN 1..294
FT /note="Transcription factor nsy-7"
FT /id="PRO_0000451152"
FT DNA_BIND 192..232
FT /note="Homeobox; atypical"
FT /evidence="ECO:0000305|PubMed:19204119"
FT REGION 43..119
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 167..191
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 238..274
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 246..263
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 136..150
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_060757"
FT MUTAGEN 179
FT /note="H->Y: In ky630; initiates but fails to maintain AWC
FT asymmetry in L1 larvae. Adults fail to express the G
FT protein-coupled receptor gene str-2 in either AWC neuron.
FT Mild defect in butanone chemotaxis, normal for chemotaxis
FT to 2,3-pentanedione."
FT /evidence="ECO:0000269|PubMed:19204119"
SQ SEQUENCE 294 AA; 33257 MW; A655D0C8CE3F080A CRC64;
MSSDTKYKYA VVRIPETSVV DFFSLIVAKG YSDVSFVASL SQCNLRDDPN DDQPTTSSNS
VKESINDDES NSENNKLSPP RRQSNPHSSL PAISSSTVKN EPTDSWTPSA LSNDPTPDLL
SATVPAELLT NLFAKTKSTE PKPQQLFGFQ ASGVDFDLSN NEWHENLRLP NGNGTEKYHP
YGGNSKNDSP LQTRMKGWQR EYIKEVIKDS HYPTEEELRD IEQKCDLSRK QILRFIAKRL
TNPNRKPRVN HHDEKRKEQE ERDSLADPDD DMINDNEAVT NLHHILNSLQ ETTA
