NSY_SOLLC
ID NSY_SOLLC Reviewed; 498 AA.
AC K4C9E2; Q9FV32; Q9LWA6;
DT 13-APR-2016, integrated into UniProtKB/Swiss-Prot.
DT 28-NOV-2012, sequence version 1.
DT 03-AUG-2022, entry version 53.
DE RecName: Full=Neoxanthin synthase, chloroplastic {ECO:0000303|PubMed:11029576};
DE EC=5.3.99.9 {ECO:0000269|PubMed:11029576};
DE AltName: Full=Lycopene beta-cyclase {ECO:0000303|PubMed:10995464};
DE EC=5.5.1.19 {ECO:0000269|PubMed:10995464};
DE Flags: Precursor;
GN Name=NSY {ECO:0000303|PubMed:11029576};
GN Synonyms=B {ECO:0000303|PubMed:10995464};
GN OrderedLocusNames=Solyc06g074240.1;
OS Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC Solanum subgen. Lycopersicon.
OX NCBI_TaxID=4081;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, AND SUBCELLULAR
RP LOCATION.
RX PubMed=11029576; DOI=10.1046/j.1432-1327.2000.01722.x;
RA Bouvier F., D'harlingue A., Backhaus R.A., Kumagai M.H., Camara B.;
RT "Identification of neoxanthin synthase as a carotenoid cyclase paralog.";
RL Eur. J. Biochem. 267:6346-6352(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, TISSUE
RP SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=10995464; DOI=10.1073/pnas.190177497;
RA Ronen G., Carmel-Goren L., Zamir D., Hirschberg J.;
RT "An alternative pathway to beta -carotene formation in plant chromoplasts
RT discovered by map-based cloning of beta and old-gold color mutations in
RT tomato.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:11102-11107(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Heinz 1706;
RX PubMed=22660326; DOI=10.1038/nature11119;
RG Tomato Genome Consortium;
RT "The tomato genome sequence provides insights into fleshy fruit
RT evolution.";
RL Nature 485:635-641(2012).
CC -!- FUNCTION: Involved in the synthesis of neoxanthin, the last product of
CC carotenoid synthesis and a precursor of abscisic acid
CC (PubMed:11029576). Involved in the beta-carotene biosynthesis
CC (PubMed:10995464). {ECO:0000269|PubMed:10995464,
CC ECO:0000269|PubMed:11029576}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=all-trans-violaxanthin = all-trans-neoxanthin;
CC Xref=Rhea:RHEA:10128, ChEBI:CHEBI:32446, ChEBI:CHEBI:35288;
CC EC=5.3.99.9; Evidence={ECO:0000269|PubMed:11029576};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a carotenoid psi-end group = a carotenoid beta-end derivative;
CC Xref=Rhea:RHEA:55620, ChEBI:CHEBI:139114, ChEBI:CHEBI:139120;
CC EC=5.5.1.19; Evidence={ECO:0000269|PubMed:10995464};
CC -!- PATHWAY: Carotenoid biosynthesis; neoxanthin biosynthesis.
CC {ECO:0000305}.
CC -!- PATHWAY: Carotenoid biosynthesis; beta-carotene biosynthesis.
CC {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255,
CC ECO:0000269|PubMed:11029576}.
CC -!- TISSUE SPECIFICITY: Expressed exclusively in chromoplast-containing
CC tissues of flowers and fruits (PubMed:10995464). Expressed in
CC preanthesis flowers (PubMed:10995464). {ECO:0000269|PubMed:10995464}.
CC -!- DEVELOPMENTAL STAGE: Expressed at the breaker stage during fruit
CC ripening. {ECO:0000269|PubMed:10995464}.
CC -!- SIMILARITY: Belongs to the lycopene cyclase family. {ECO:0000305}.
CC -!- CAUTION: A unique gene in the tomato genome is described to encode for
CC a protein with either a lycopene beta-cyclase activity or an exclusive
CC neoxanthin synthase activity with no apparent lycopene beta-cyclase
CC activity. According to a report, has lycopene beta-cyclase activity
CC (PubMed:10995464). According to another report, displays exclusive
CC neoxanthin synthase activity with no apparent lycopene beta-cyclase
CC activity (PubMed:11029576). This discrepancy might be due to the
CC differences in the cultivars used for the gene cloning and/or in the
CC methods used to determine the enzymatic activities, in vitro assay or
CC molecular analysis of mutants. {ECO:0000305|PubMed:10995464,
CC ECO:0000305|PubMed:11029576}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; Y18297; CAB93342.1; -; mRNA.
DR EMBL; AF254793; AAG21133.1; -; mRNA.
DR RefSeq; NP_001234445.2; NM_001247516.2.
DR AlphaFoldDB; K4C9E2; -.
DR SMR; K4C9E2; -.
DR STRING; 4081.Solyc06g074240.1.1; -.
DR PaxDb; K4C9E2; -.
DR PRIDE; K4C9E2; -.
DR GeneID; 543649; -.
DR KEGG; sly:543649; -.
DR eggNOG; ENOG502QQD7; Eukaryota.
DR HOGENOM; CLU_032956_1_0_1; -.
DR InParanoid; K4C9E2; -.
DR OrthoDB; 815606at2759; -.
DR PhylomeDB; K4C9E2; -.
DR BioCyc; MetaCyc:MON-12165; -.
DR BRENDA; 5.3.99.9; 3101.
DR UniPathway; UPA00388; -.
DR UniPathway; UPA00802; -.
DR Proteomes; UP000004994; Unplaced.
DR ExpressionAtlas; K4C9E2; baseline and differential.
DR GO; GO:0009507; C:chloroplast; IDA:UniProtKB.
DR GO; GO:0034020; F:neoxanthin synthase activity; IDA:UniProtKB.
DR GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR GO; GO:0016120; P:carotene biosynthetic process; IBA:GO_Central.
DR GO; GO:0006744; P:ubiquinone biosynthetic process; IBA:GO_Central.
DR GO; GO:0016123; P:xanthophyll biosynthetic process; IDA:UniProtKB.
DR Gene3D; 3.50.50.60; -; 1.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR010108; Lycopene_cyclase_b/e.
DR SUPFAM; SSF51905; SSF51905; 1.
DR TIGRFAMs; TIGR01790; carotene-cycl; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Isomerase; Plastid; Reference proteome; Transit peptide.
FT TRANSIT 1..42
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 43..498
FT /note="Neoxanthin synthase, chloroplastic"
FT /evidence="ECO:0000255"
FT /id="PRO_0000435898"
FT CONFLICT 3
FT /note="T -> A (in Ref. 2; AAG21133)"
FT /evidence="ECO:0000305"
FT CONFLICT 19
FT /note="Y -> H (in Ref. 2; AAG21133)"
FT /evidence="ECO:0000305"
FT CONFLICT 23
FT /note="V -> F (in Ref. 2; AAG21133)"
FT /evidence="ECO:0000305"
FT CONFLICT 136
FT /note="D -> N (in Ref. 2; AAG21133)"
FT /evidence="ECO:0000305"
FT CONFLICT 219
FT /note="G -> D (in Ref. 1; CAB93342)"
FT /evidence="ECO:0000305"
FT CONFLICT 490
FT /note="I -> M (in Ref. 1; CAB93342)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 498 AA; 56458 MW; 1C4D3B5DAD9B6B40 CRC64;
METLLKPFPS LLLSSPTPYR SIVQQNPSFL SPTTKKKSRK CLLRNKSSKL FCSFLDLAPT
SKPESLDVNI SWVDPNSNRA QFDVIIIGAG PAGLRLAEQV SKYGIKVCCV DPSPLSMWPN
NYGVWVDEFE NLGLEDCLDH KWPMTCVHIN DNKTKYLGRP YGRVSRKKLK LKLLNSCVEN
RVKFYKAKVW KVEHEEFESS IVCDDGKKIR GSLVVDASGF ASDFIEYDRP RNHGYQIAHG
VLVEVDNHPF DLDKMVLMDW RDSHLGNEPY LRVNNAKEPT FLYAMPFDRD LVFLEETSLV
SRPVLSYMEV KRRMVARLRH LGIKVKSVIE EEKCVIPMGG PLPRIPQNVM AIGGNSGIVH
PSTGYMVARS MALAPVLAEA IVEGLGSTRM IRGSQLYHRV WNGLWPLDRR CVRECYSFGM
ETLLKLDLKG TRRLFDAFFD LDPKYWQGFL SSRLSVKELG LLSLCLFGHG SNMTRLDIVT
KCPLPLVRLI GNLAIESL
