NT56_DROME
ID NT56_DROME Reviewed; 510 AA.
AC Q27333; O02532; O02533; Q24419; Q24420; Q24421; Q24607; Q95R76; Q9W1K8;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Lethal(2)neighbour of tid protein;
DE AltName: Full=NOT45;
DE AltName: Full=NOT56;
GN Name=l(2)not; ORFNames=CG4084;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND CHARACTERIZATION.
RC STRAIN=Apxo, bIf, Harvich, and Oregon-R; TISSUE=Embryo;
RX PubMed=9373138; DOI=10.1016/s0378-1119(97)00373-9;
RA Kurzik-Dumke U., Kaymer M., Gundacker D., Debes A., Labitzke K.;
RT "Gene within gene configuration and expression of the Drosophila
RT melanogaster genes lethal(2) neighbour of tid [l(2)not] and lethal(2)
RT relative of tid.";
RL Gene 200:45-58(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Oregon-2;
RA Gundacker S., Neubhuer M., Kurzik-Dumke U.;
RT "Sequence of a 10291 nt genomic region of Drosophila melanogaster
RT harbouring the genes l(2)tid, l(2)not, l(2)rot, and l(2)dtl.";
RL Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 179-510.
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305};
CC Multi-pass membrane protein {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Expressed only in embryos.
CC -!- MISCELLANEOUS: In strains Harvich and Apxo, there are 2 genes which
CC code for l(2)not protein. They differ in their C-terminus.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 58 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL29130.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; X95241; CAA64529.1; -; Genomic_DNA.
DR EMBL; X77820; CAA54835.1; -; mRNA.
DR EMBL; X95248; CAA64537.1; -; Genomic_DNA.
DR EMBL; X95252; CAA64541.1; -; Genomic_DNA.
DR EMBL; X95243; CAA64532.1; -; Genomic_DNA.
DR EMBL; Y10074; CAA71167.1; -; Genomic_DNA.
DR EMBL; X98094; CAA66721.1; -; Genomic_DNA.
DR EMBL; AE013599; AAF47050.1; -; Genomic_DNA.
DR EMBL; AY061582; AAL29130.1; ALT_INIT; mRNA.
DR PIR; S42090; S42090.
DR RefSeq; NP_523829.2; NM_079105.4.
DR AlphaFoldDB; Q27333; -.
DR SMR; Q27333; -.
DR BioGRID; 63350; 7.
DR IntAct; Q27333; 1.
DR STRING; 7227.FBpp0072004; -.
DR CAZy; GT58; Glycosyltransferase Family 58.
DR PaxDb; Q27333; -.
DR PRIDE; Q27333; -.
DR DNASU; 37754; -.
DR GeneID; 37754; -.
DR KEGG; dme:Dmel_CG4084; -.
DR CTD; 10195; -.
DR FlyBase; FBgn0011297; l(2)not.
DR VEuPathDB; VectorBase:FBgn0011297; -.
DR eggNOG; KOG2762; Eukaryota.
DR HOGENOM; CLU_035382_3_0_1; -.
DR InParanoid; Q27333; -.
DR PhylomeDB; Q27333; -.
DR Reactome; R-DME-446193; Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.
DR SignaLink; Q27333; -.
DR BioGRID-ORCS; 37754; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 37754; -.
DR PRO; PR:Q27333; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR ExpressionAtlas; Q27333; baseline and differential.
DR Genevisible; Q27333; DM.
DR GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0052925; F:dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase activity; IBA:GO_Central.
DR GO; GO:0010804; P:negative regulation of tumor necrosis factor-mediated signaling pathway; IGI:FlyBase.
DR GO; GO:0006486; P:protein glycosylation; IBA:GO_Central.
DR InterPro; IPR007873; Glycosyltransferase_ALG3.
DR PANTHER; PTHR12646; PTHR12646; 1.
DR Pfam; PF05208; ALG3; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Endoplasmic reticulum; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..510
FT /note="Lethal(2)neighbour of tid protein"
FT /id="PRO_0000080568"
FT TRANSMEM 44..64
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 98..118
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 126..146
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 172..192
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 212..232
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 234..254
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 295..315
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 404..424
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 427..447
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 465..485
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 337..358
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 342..358
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 286
FT /note="V -> L (in strain: Berkeley and bIf)"
FT VARIANT 311
FT /note="I -> T (in strain: Berkeley, bIf and Apxo)"
FT VARIANT 333
FT /note="T -> A (in strain: Berkeley, bIf and Apxo)"
FT VARIANT 338
FT /note="Q -> E (in strain: Berkeley, bIf and Apxo)"
FT VARIANT 340
FT /note="K -> N (in strain: Berkeley and bIf)"
FT VARIANT 489
FT /note="I -> V (in strain: Berkeley, bIf and Apxo)"
FT MUTAGEN 433
FT /note="H->R: In tid4 mutant."
SQ SEQUENCE 510 AA; 58461 MW; E2F7415940465F25 CRC64;
MAPPKAASHR PAVRRKKSGT LVDSILDKYL NVRFFKYLLL EPAALPIVGL FVLLAELVIN
VVVIQRVPYT EIDWVAYMQE CEGFLNGTTN YSLLRGDTGP LVYPAAFVYI YSALYYVTSH
GTNVRLAQYI FAGIYLLQLA LVLRLYSKSR KVPPYVLVLS AFTSYRIHSI YVLRLFNDPV
AVLLLYAALN LFLDRRWTLG STFFSLAVGV KMNILLFAPA LLLFYLANLG LLRTILQLAV
CGVIQLLLGA PFLLTHPVEY LRGSFDLGRI FEHKWTVNYR FLSRDVFENR TFHVSLLGLH
LLLLLAFAKP IWTFFQSYVR LRRIEDQLQP QITQQNLQLK AQKRPKKVEK DKDKDQKKFT
TEQQSFLKAF EKSLQKASGG KATPAPAQAE PERYGIHFDR CTQLALLPFF LCNLVGVACS
RSLHYQFYVW YFHSLPYLAW STPYSLGVRC LILGLIEYCW NTYPSTNFSS AALHFTHIIL
LAGVAKQLIQ TMRINNAAKR EQQEQQKKLQ
