NT5D1_MOUSE
ID NT5D1_MOUSE Reviewed; 467 AA.
AC Q8C5P5; Q3TAL3; Q3TPT8; Q3TTI3; Q3UL73; Q4G0D1;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 25-JUL-2006, sequence version 2.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=5'-nucleotidase domain-containing protein 1;
DE EC=3.1.3.-;
DE AltName: Full=Cytosolic 5'-nucleotidase II-like protein 1;
GN Name=Nt5dc1; Synonyms=Nt5c2l1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
RC STRAIN=C57BL/6J, and NOD; TISSUE=Hippocampus, Spleen, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Egg;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8C5P5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8C5P5-2; Sequence=VSP_019948;
CC Name=3;
CC IsoId=Q8C5P5-3; Sequence=VSP_019949, VSP_019950;
CC -!- SIMILARITY: Belongs to the 5'(3')-deoxyribonucleotidase family.
CC {ECO:0000305}.
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DR EMBL; AK077876; BAC37046.1; -; mRNA.
DR EMBL; AK145666; BAE26577.1; -; mRNA.
DR EMBL; AK161350; BAE36342.1; -; mRNA.
DR EMBL; AK164144; BAE37647.1; -; mRNA.
DR EMBL; AK171761; BAE42655.1; -; mRNA.
DR EMBL; BC098476; AAH98476.1; -; mRNA.
DR CCDS; CCDS23779.1; -. [Q8C5P5-1]
DR RefSeq; NP_001334392.1; NM_001347463.1.
DR RefSeq; NP_795942.2; NM_176968.5. [Q8C5P5-1]
DR AlphaFoldDB; Q8C5P5; -.
DR SMR; Q8C5P5; -.
DR BioGRID; 235414; 1.
DR STRING; 10090.ENSMUSP00000047126; -.
DR iPTMnet; Q8C5P5; -.
DR PhosphoSitePlus; Q8C5P5; -.
DR EPD; Q8C5P5; -.
DR MaxQB; Q8C5P5; -.
DR PaxDb; Q8C5P5; -.
DR PRIDE; Q8C5P5; -.
DR ProteomicsDB; 295533; -. [Q8C5P5-1]
DR ProteomicsDB; 295534; -. [Q8C5P5-2]
DR ProteomicsDB; 295535; -. [Q8C5P5-3]
DR Antibodypedia; 32478; 207 antibodies from 17 providers.
DR DNASU; 319638; -.
DR Ensembl; ENSMUST00000047885; ENSMUSP00000047126; ENSMUSG00000039480. [Q8C5P5-1]
DR Ensembl; ENSMUST00000105512; ENSMUSP00000101151; ENSMUSG00000039480. [Q8C5P5-2]
DR GeneID; 319638; -.
DR KEGG; mmu:319638; -.
DR UCSC; uc007euv.1; mouse. [Q8C5P5-1]
DR UCSC; uc007euw.1; mouse. [Q8C5P5-2]
DR CTD; 221294; -.
DR MGI; MGI:2442446; Nt5dc1.
DR VEuPathDB; HostDB:ENSMUSG00000039480; -.
DR eggNOG; KOG2469; Eukaryota.
DR GeneTree; ENSGT00940000155676; -.
DR HOGENOM; CLU_029966_0_0_1; -.
DR InParanoid; Q8C5P5; -.
DR OMA; NWHQLHA; -.
DR PhylomeDB; Q8C5P5; -.
DR TreeFam; TF325912; -.
DR BioGRID-ORCS; 319638; 2 hits in 72 CRISPR screens.
DR PRO; PR:Q8C5P5; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; Q8C5P5; protein.
DR Bgee; ENSMUSG00000039480; Expressed in choroid plexus epithelium and 200 other tissues.
DR ExpressionAtlas; Q8C5P5; baseline and differential.
DR Genevisible; Q8C5P5; MM.
DR GO; GO:0008253; F:5'-nucleotidase activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.1000; -; 1.
DR InterPro; IPR036412; HAD-like_sf.
DR InterPro; IPR008380; HAD-SF_hydro_IG_5-nucl.
DR InterPro; IPR023214; HAD_sf.
DR PANTHER; PTHR12103; PTHR12103; 1.
DR Pfam; PF05761; 5_nucleotid; 1.
DR SUPFAM; SSF56784; SSF56784; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Hydrolase; Magnesium; Metal-binding;
KW Reference proteome.
FT CHAIN 1..467
FT /note="5'-nucleotidase domain-containing protein 1"
FT /id="PRO_0000247223"
FT ACT_SITE 16
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 18
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT BINDING 16
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 18
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 323
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT MOD_RES 181
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q5TFE4"
FT VAR_SEQ 159
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_019948"
FT VAR_SEQ 188..201
FT /note="NCGFFFPEIKRNLG -> PGMCPSPHDRETSP (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_019949"
FT VAR_SEQ 202..467
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_019950"
FT CONFLICT 8
FT /note="G -> A (in Ref. 1; BAE42655)"
FT /evidence="ECO:0000305"
FT CONFLICT 26
FT /note="L -> P (in Ref. 2; AAH98476)"
FT /evidence="ECO:0000305"
FT CONFLICT 42
FT /note="V -> I (in Ref. 1; BAE37647)"
FT /evidence="ECO:0000305"
FT CONFLICT 178
FT /note="H -> P (in Ref. 1; BAE36342)"
FT /evidence="ECO:0000305"
FT CONFLICT 197
FT /note="K -> N (in Ref. 2; AAH98476)"
FT /evidence="ECO:0000305"
FT CONFLICT 266
FT /note="S -> P (in Ref. 2; AAH98476)"
FT /evidence="ECO:0000305"
FT CONFLICT 300
FT /note="A -> V (in Ref. 1; BAE42655)"
FT /evidence="ECO:0000305"
FT CONFLICT 364
FT /note="R -> K (in Ref. 1; BAC37046)"
FT /evidence="ECO:0000305"
FT CONFLICT 371
FT /note="M -> T (in Ref. 1; BAE42655)"
FT /evidence="ECO:0000305"
FT CONFLICT 377
FT /note="T -> S (in Ref. 1; BAE42655)"
FT /evidence="ECO:0000305"
FT CONFLICT 400
FT /note="V -> L (in Ref. 1; BAE42655)"
FT /evidence="ECO:0000305"
FT CONFLICT 442
FT /note="M -> V (in Ref. 1; BAE42655)"
FT /evidence="ECO:0000305"
FT CONFLICT 451
FT /note="H -> Y (in Ref. 1; BAE42655)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 467 AA; 53094 MW; B9A4F2307BA4B32F CRC64;
MAQHFSLGAC DVVGFDLDHT LCRYNLPESA RLIYNSFAQF LVKEKGYDEG LLTLTPEDWD
FCCKGLALDL EDGTFIKLAA DGTVLRASHG TKMMTPEALT EAFGKKEWRH CVSDKRCTSD
KPGVSDIPCC SGKCYFYDNY FDLPGALLCA RVVDSLTKQN RGQKTFDFWK DVVAGIQHNF
KMSAFKENCG FFFPEIKRNL GKYVHRCPES VRKWLRQLKD AGKITMLITS SHSDYCKLLG
SYILGEDFAD LFDIVITNAL KPGFFSHFPS QRPFYTLEND EEKDELPSLD KPGWYSQGNA
AHLYELLKKM TSKPEPKVVY FGDSMHSDIF PAHHYTNWET VLILEELQGP EMEKPEEAEP
LEKRGKYEAP MVKPLNTLSN KWGSYFIDSV SGRGRAEDSV VYTWSSKRIS TYSTIAIPNI
ESIAELPLDY KFTRFSTNNS KMAGYYPLVH HTLGSQDTDS KIISTEK
