NT5D3_HUMAN
ID NT5D3_HUMAN Reviewed; 548 AA.
AC Q86UY8; Q9NUM7; Q9P2T2; Q9P2T3;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=5'-nucleotidase domain-containing protein 3;
DE EC=3.1.3.-;
DE AltName: Full=GRP94-neighboring nucleotidase;
GN Name=NT5DC3; Synonyms=GNN, TU12B1-TY;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [MRNA]
RP OF 51-548 (ISOFORM 1), AND TISSUE SPECIFICITY.
RX PubMed=15547748;
RA Yatsuoka T., Furukawa T., Sunamura M., Matsuno S., Horii A.;
RT "TU12B1-TY, a novel gene in the region at 12q22-q23.1 frequently deleted in
RT pancreatic cancer, shows reduced expression in pancreatic cancer cells.";
RL Oncol. Rep. 12:1263-1268(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Skin;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 428-548 (ISOFORM 1).
RC TISSUE=Placenta;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=25944712; DOI=10.1002/pmic.201400617;
RA Vaca Jacome A.S., Rabilloud T., Schaeffer-Reiss C., Rompais M., Ayoub D.,
RA Lane L., Bairoch A., Van Dorsselaer A., Carapito C.;
RT "N-terminome analysis of the human mitochondrial proteome.";
RL Proteomics 15:2519-2524(2015).
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000250};
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q86UY8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q86UY8-2; Sequence=VSP_032643, VSP_032644;
CC -!- TISSUE SPECIFICITY: Expressed in brain, placenta, skeletal muscle,
CC pancreas, testis, uterus, and small intestine. Reduced expression in
CC pancreatic cancer cells. {ECO:0000269|PubMed:15547748}.
CC -!- SIMILARITY: Belongs to the 5'(3')-deoxyribonucleotidase family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA92095.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAA93053.1; Type=Frameshift; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB032773; BAA93053.1; ALT_FRAME; mRNA.
DR EMBL; AB032786; BAA93086.1; -; Genomic_DNA.
DR EMBL; CH471054; EAW97716.1; -; Genomic_DNA.
DR EMBL; BC052293; AAH52293.1; -; mRNA.
DR EMBL; AK002128; BAA92095.1; ALT_INIT; mRNA.
DR CCDS; CCDS41824.1; -. [Q86UY8-1]
DR RefSeq; NP_001026871.1; NM_001031701.2. [Q86UY8-1]
DR AlphaFoldDB; Q86UY8; -.
DR SMR; Q86UY8; -.
DR BioGRID; 119609; 22.
DR IntAct; Q86UY8; 12.
DR STRING; 9606.ENSP00000376615; -.
DR DEPOD; NT5DC3; -.
DR iPTMnet; Q86UY8; -.
DR PhosphoSitePlus; Q86UY8; -.
DR BioMuta; NT5DC3; -.
DR DMDM; 74727508; -.
DR EPD; Q86UY8; -.
DR jPOST; Q86UY8; -.
DR MassIVE; Q86UY8; -.
DR MaxQB; Q86UY8; -.
DR PaxDb; Q86UY8; -.
DR PeptideAtlas; Q86UY8; -.
DR PRIDE; Q86UY8; -.
DR ProteomicsDB; 69939; -. [Q86UY8-1]
DR ProteomicsDB; 69940; -. [Q86UY8-2]
DR Antibodypedia; 48099; 28 antibodies from 9 providers.
DR DNASU; 51559; -.
DR Ensembl; ENST00000392876.8; ENSP00000376615.3; ENSG00000111696.12. [Q86UY8-1]
DR GeneID; 51559; -.
DR KEGG; hsa:51559; -.
DR MANE-Select; ENST00000392876.8; ENSP00000376615.3; NM_001031701.3; NP_001026871.1.
DR UCSC; uc010swe.2; human. [Q86UY8-1]
DR CTD; 51559; -.
DR DisGeNET; 51559; -.
DR GeneCards; NT5DC3; -.
DR HGNC; HGNC:30826; NT5DC3.
DR HPA; ENSG00000111696; Tissue enhanced (intestine).
DR MIM; 611076; gene.
DR neXtProt; NX_Q86UY8; -.
DR OpenTargets; ENSG00000111696; -.
DR PharmGKB; PA143485562; -.
DR VEuPathDB; HostDB:ENSG00000111696; -.
DR eggNOG; KOG2470; Eukaryota.
DR GeneTree; ENSGT00940000158637; -.
DR HOGENOM; CLU_017845_5_1_1; -.
DR OMA; SCMLNYD; -.
DR OrthoDB; 858908at2759; -.
DR PhylomeDB; Q86UY8; -.
DR TreeFam; TF323990; -.
DR PathwayCommons; Q86UY8; -.
DR SignaLink; Q86UY8; -.
DR BioGRID-ORCS; 51559; 15 hits in 1075 CRISPR screens.
DR ChiTaRS; NT5DC3; human.
DR GenomeRNAi; 51559; -.
DR Pharos; Q86UY8; Tdark.
DR PRO; PR:Q86UY8; -.
DR Proteomes; UP000005640; Chromosome 12.
DR RNAct; Q86UY8; protein.
DR Bgee; ENSG00000111696; Expressed in muscle layer of sigmoid colon and 126 other tissues.
DR ExpressionAtlas; Q86UY8; baseline and differential.
DR Genevisible; Q86UY8; HS.
DR GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR GO; GO:0043235; C:receptor complex; IDA:MGI.
DR GO; GO:0008253; F:5'-nucleotidase activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.1000; -; 1.
DR InterPro; IPR036412; HAD-like_sf.
DR InterPro; IPR008380; HAD-SF_hydro_IG_5-nucl.
DR InterPro; IPR023214; HAD_sf.
DR InterPro; IPR016695; Pur_nucleotidase.
DR PANTHER; PTHR12103; PTHR12103; 1.
DR Pfam; PF05761; 5_nucleotid; 1.
DR PIRSF; PIRSF017434; Purine_5'-nucleotidase; 1.
DR SUPFAM; SSF56784; SSF56784; 1.
DR TIGRFAMs; TIGR02244; HAD-IG-Ncltidse; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Hydrolase; Magnesium; Metal-binding;
KW Reference proteome.
FT CHAIN 1..548
FT /note="5'-nucleotidase domain-containing protein 3"
FT /id="PRO_0000326247"
FT REGION 528..548
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 102
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 104
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT BINDING 102
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 104
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 251..259
FT /ligand="substrate"
FT /evidence="ECO:0000255"
FT BINDING 389
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT VAR_SEQ 1..75
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_032643"
FT VAR_SEQ 466..474
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_032644"
FT VARIANT 266
FT /note="A -> E (in dbSNP:rs12184494)"
FT /id="VAR_040043"
SQ SEQUENCE 548 AA; 63420 MW; 93B1C8CD7C2E0434 CRC64;
MTMAAAAVVA RGAGARAATA AALRGGCGTA ARGRPCAGPA RPLCTAPGTA PDMKRYLWER
YREAKRSTEE LVPSIMSNLL NPDAIFSNNE MSLSDIEIYG FDYDYTLVFY SKHLHTLIFN
AARDLLINEH RYPAEIRKYE YDPNFAIRGL HYDVQRAVLM KIDAFHYIQL GTVYRGLSVV
PDEEVIEMYE GSHVPLEQMS DFYGKSSHGN TMKQFMDIFS LPEMTLLSCV NEYFLKNNID
YEPVHLYKDV KDSIRDVHIK GIMYRAIEAD IEKYICYAEQ TRAVLAKLAD HGKKMFLITN
SPSSFVDKGM SYIVGKDWRD LFDVVIVQAE KPNFFNDKRR PFRKMNEKGV LLWDKIHKLQ
KGQIYKQGNL YEFLKLTGWR GSRVLYFGDH IYSDLADLTL KHGWRTGAII PELRSELKIM
NTEQYIQTMT WLQTLTGLLE QMQVHRDAES QLVLQEWKKE RKEMREMTKS FFNAQFGSLF
RTDQNPTYFL RRLSRFADIY MASLSCLLNY DVSHTFYPRR TPLQHELPAW SERPPTFGTP
LLQEAQAK
