NT5D3_MOUSE
ID NT5D3_MOUSE Reviewed; 546 AA.
AC Q3UHB1; Q3T9W5;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=5'-nucleotidase domain-containing protein 3;
DE EC=3.1.3.-;
DE AltName: Full=GRP94-neighboring nucleotidase;
GN Name=Nt5dc3; Synonyms=Gnn;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=NOD; TISSUE=Spleen;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Heart, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000250};
CC -!- SIMILARITY: Belongs to the 5'(3')-deoxyribonucleotidase family.
CC {ECO:0000305}.
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DR EMBL; AK147488; BAE27946.1; -; mRNA.
DR EMBL; AK172248; BAE42905.1; -; mRNA.
DR CCDS; CCDS36020.1; -.
DR RefSeq; NP_780540.2; NM_175331.3.
DR AlphaFoldDB; Q3UHB1; -.
DR SMR; Q3UHB1; -.
DR BioGRID; 222087; 3.
DR IntAct; Q3UHB1; 1.
DR MINT; Q3UHB1; -.
DR STRING; 10090.ENSMUSP00000096994; -.
DR iPTMnet; Q3UHB1; -.
DR PhosphoSitePlus; Q3UHB1; -.
DR SwissPalm; Q3UHB1; -.
DR EPD; Q3UHB1; -.
DR MaxQB; Q3UHB1; -.
DR PaxDb; Q3UHB1; -.
DR PeptideAtlas; Q3UHB1; -.
DR PRIDE; Q3UHB1; -.
DR ProteomicsDB; 253029; -.
DR DNASU; 103466; -.
DR Ensembl; ENSMUST00000099396; ENSMUSP00000096994; ENSMUSG00000054027.
DR GeneID; 103466; -.
DR KEGG; mmu:103466; -.
DR UCSC; uc007gqm.1; mouse.
DR CTD; 51559; -.
DR MGI; MGI:3513266; Nt5dc3.
DR VEuPathDB; HostDB:ENSMUSG00000054027; -.
DR eggNOG; KOG2470; Eukaryota.
DR GeneTree; ENSGT00940000158637; -.
DR HOGENOM; CLU_017845_5_1_1; -.
DR InParanoid; Q3UHB1; -.
DR OMA; NEAMAPW; -.
DR OrthoDB; 858908at2759; -.
DR PhylomeDB; Q3UHB1; -.
DR TreeFam; TF323990; -.
DR BioGRID-ORCS; 103466; 2 hits in 72 CRISPR screens.
DR ChiTaRS; Nt5dc3; mouse.
DR PRO; PR:Q3UHB1; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; Q3UHB1; protein.
DR Bgee; ENSMUSG00000054027; Expressed in medial dorsal nucleus of thalamus and 231 other tissues.
DR Genevisible; Q3UHB1; MM.
DR GO; GO:0005829; C:cytosol; IDA:MGI.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR GO; GO:0043235; C:receptor complex; ISO:MGI.
DR GO; GO:0008253; F:5'-nucleotidase activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.1000; -; 1.
DR InterPro; IPR036412; HAD-like_sf.
DR InterPro; IPR008380; HAD-SF_hydro_IG_5-nucl.
DR InterPro; IPR023214; HAD_sf.
DR InterPro; IPR016695; Pur_nucleotidase.
DR PANTHER; PTHR12103; PTHR12103; 1.
DR Pfam; PF05761; 5_nucleotid; 1.
DR PIRSF; PIRSF017434; Purine_5'-nucleotidase; 1.
DR SUPFAM; SSF56784; SSF56784; 1.
DR TIGRFAMs; TIGR02244; HAD-IG-Ncltidse; 1.
PE 1: Evidence at protein level;
KW Hydrolase; Magnesium; Metal-binding; Reference proteome.
FT CHAIN 1..546
FT /note="5'-nucleotidase domain-containing protein 3"
FT /id="PRO_0000326248"
FT ACT_SITE 100
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 102
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT BINDING 100
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 102
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 249..257
FT /ligand="substrate"
FT /evidence="ECO:0000255"
FT BINDING 387
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT CONFLICT 75
FT /note="N -> D (in Ref. 1; BAE42905)"
FT /evidence="ECO:0000305"
FT CONFLICT 157
FT /note="L -> W (in Ref. 1; BAE42905)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 546 AA; 63170 MW; 4D3C8BAA1D61161C CRC64;
MATVAAAARG AGARAAAGLR SCGGAVARER PRSGCARRLC SAPAAPAAVD MKSYLWARYH
EAKRSTDELV PSIMNNLLNP DAIFSNNEMS LSDIEIYGFD YDYTLVFYSK HLHTLIFNAA
RDLLINEHRY PVEIRKYEYD PSFAIRGLHY DVQRAVLMKI DAFHYIQMGT VYRGLSVVPD
EEVIDMYEGS HVPLEQMSDF YGKSSHGNTM KQFMDIFSLP EMTLLSCVNE HFLKNNIDYE
PVHLYKDVKD SIRDVHIKGI MYRAIEADIE KYICYADQTR AVLAKLAAHG KKMFLITNSP
SSFVDKGMRY IVGKDWRDLF DVVIVQAEKP NFFNDKRRPF RKVNEKGVLL WDKIHKLQKG
QIYKQGNLYE FLKLTGWRGS KVLYFGDHIY SDLADLTLKH GWRTGAIIPE LRSELRIMNT
EQYIQTMTWL QTLTGLLEQM QVHRDAESQL VLQEWKKERK EMREMTKSFF NAQFGSLFRT
DQNPTYFLRR LSRFADIYMA SLSCLLNYDV HHTFYPRRTP LQHELPAWSD SPSAFKAPLL
QEAQAK
