ID   NT5D3_XENTR             Reviewed;         534 AA.
AC   A4IHT9; A9UMH4;
DT   18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2007, sequence version 1.
DT   03-AUG-2022, entry version 66.
DE   RecName: Full=5'-nucleotidase domain-containing protein 3;
DE            EC=3.1.3.-;
GN   Name=nt5dc3;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND NUCLEOTIDE SEQUENCE
RP   [LARGE SCALE MRNA] OF 13-534 (ISOFORM 2).
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000250};
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=A4IHT9-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=A4IHT9-2; Sequence=VSP_032645, VSP_032646;
CC   -!- SIMILARITY: Belongs to the 5'(3')-deoxyribonucleotidase family.
CC       {ECO:0000305}.
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DR   EMBL; BC135684; AAI35685.1; -; mRNA.
DR   EMBL; BC157658; AAI57659.1; -; mRNA.
DR   RefSeq; NP_001096314.1; NM_001102844.1. [A4IHT9-1]
DR   AlphaFoldDB; A4IHT9; -.
DR   SMR; A4IHT9; -.
DR   STRING; 8364.ENSXETP00000011758; -.
DR   PaxDb; A4IHT9; -.
DR   DNASU; 100124894; -.
DR   GeneID; 100124894; -.
DR   KEGG; xtr:100124894; -.
DR   CTD; 51559; -.
DR   Xenbase; XB-GENE-940463; nt5dc3.
DR   eggNOG; KOG2470; Eukaryota.
DR   InParanoid; A4IHT9; -.
DR   OrthoDB; 858908at2759; -.
DR   Proteomes; UP000008143; Chromosome 3.
DR   Proteomes; UP000790000; Unplaced.
DR   GO; GO:0008253; F:5'-nucleotidase activity; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1000; -; 1.
DR   InterPro; IPR036412; HAD-like_sf.
DR   InterPro; IPR008380; HAD-SF_hydro_IG_5-nucl.
DR   InterPro; IPR023214; HAD_sf.
DR   InterPro; IPR016695; Pur_nucleotidase.
DR   PANTHER; PTHR12103; PTHR12103; 1.
DR   Pfam; PF05761; 5_nucleotid; 1.
DR   PIRSF; PIRSF017434; Purine_5'-nucleotidase; 1.
DR   SUPFAM; SSF56784; SSF56784; 1.
DR   TIGRFAMs; TIGR02244; HAD-IG-Ncltidse; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Hydrolase; Magnesium; Metal-binding;
KW   Reference proteome.
FT   CHAIN           1..534
FT                   /note="5'-nucleotidase domain-containing protein 3"
FT                   /id="PRO_0000326250"
FT   ACT_SITE        104
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        106
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   BINDING         104
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         106
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         234..242
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255"
FT   BINDING         372
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   VAR_SEQ         33..70
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|Ref.1"
FT                   /id="VSP_032645"
FT   VAR_SEQ         184
FT                   /note="D -> DSEVIEMYEGSHVPLEQMSD (in isoform 2)"
FT                   /evidence="ECO:0000303|Ref.1"
FT                   /id="VSP_032646"
SQ   SEQUENCE   534 AA;  62356 MW;  E2ECF6F6670CEAEE CRC64;
     MTSAVPLLSR WCRATLAARK KLTHKAEPAF CKGIWWQCVR SLAALSNGPE TGDMRGYLTS
     NYKEVKRSIN EELVPSVSSS LLNPDAIFAN NEMSLEDIEV YGFDYDYTLA FYSKDLHTLI
     FNTARDLLIN EHRYPKEIRS YEYDPNFAIR GLHYDVHKAL LMKIDSFHYI QLGTVYRGLN
     VVPDFYGKSS EGNTLKQFMD IFSLPEMTLL SCVNEYFLKN NIDYEPVHLY KDVKEAIRDV
     HVKGILYRAV EADIERYICY GERTQAVLSK LANLGKKMFL ITNSPSSFVN KGMNFIVGKD
     WRDLFDVVIV QADKPNFFND KRRPFRKVAE RGILLWDKIH HLEKGQTYKQ GNLFEFLKLT
     GWRGSKVLYF GDHIYSDLAD LTLKHGWRTG AIIPELKSEI EIMNTSHYIT TVTWLQGLTG
     LLERMQVYRD AESQMVLQDW IKERQEMRDL ARKMFNRQFG SIFRTDHNPT YFLRRLSRFA
     DIYMESLSCL LNYDLHHTFY PKRTPLQHEL PVCSEQQNTG TFRIPLGKEM AQIK