ID   NT5D_DICDI              Reviewed;         591 AA.
AC   Q75K12; Q552N5;
DT   16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=5'-nucleotidase domain-containing protein DDB_G0275467;
DE            EC=3.1.3.-;
GN   ORFNames=DDB_G0275467;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=12097910; DOI=10.1038/nature00847;
RA   Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA   Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA   Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA   Noegel A.A.;
RT   "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL   Nature 418:79-85(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000305};
CC       Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000305};
CC   -!- SIMILARITY: Belongs to the 5'(3')-deoxyribonucleotidase family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AAFI02000013; EAL69484.1; -; Genomic_DNA.
DR   RefSeq; XP_643646.1; XM_638554.1.
DR   AlphaFoldDB; Q75K12; -.
DR   SMR; Q75K12; -.
DR   STRING; 44689.DDB0230203; -.
DR   PaxDb; Q75K12; -.
DR   EnsemblProtists; EAL69484; EAL69484; DDB_G0275467.
DR   GeneID; 8620233; -.
DR   KEGG; ddi:DDB_G0275467; -.
DR   dictyBase; DDB_G0275467; -.
DR   eggNOG; KOG2470; Eukaryota.
DR   HOGENOM; CLU_017845_5_1_1; -.
DR   InParanoid; Q75K12; -.
DR   OMA; SCMLNYD; -.
DR   PhylomeDB; Q75K12; -.
DR   PRO; PR:Q75K12; -.
DR   Proteomes; UP000002195; Chromosome 2.
DR   GO; GO:0008253; F:5'-nucleotidase activity; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1000; -; 1.
DR   InterPro; IPR036412; HAD-like_sf.
DR   InterPro; IPR008380; HAD-SF_hydro_IG_5-nucl.
DR   InterPro; IPR023214; HAD_sf.
DR   PANTHER; PTHR12103; PTHR12103; 1.
DR   Pfam; PF05761; 5_nucleotid; 1.
DR   SUPFAM; SSF56784; SSF56784; 1.
DR   TIGRFAMs; TIGR02244; HAD-IG-Ncltidse; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Magnesium; Metal-binding; Reference proteome.
FT   CHAIN           1..591
FT                   /note="5'-nucleotidase domain-containing protein
FT                   DDB_G0275467"
FT                   /id="PRO_0000356850"
FT   REGION          38..88
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        165
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        167
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255"
FT   BINDING         165
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255"
FT   BINDING         167
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255"
FT   BINDING         305..313
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255"
FT   BINDING         450
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   591 AA;  68462 MW;  2830D28A444A3D8E CRC64;
     MLSNKRISLI KVLPIPSNKK NVFKINCSSN TTIITTSSTT TTSGIKSYST HNRSNNDTHT
     SKSNTIDQHQ QQQPQQHQNN DNKHLFTPTP FQSLVKNVNK PFRNDKDPDY LPTHHSVLNM
     SLDQLIEMYK NRKVVDLSHV PTLDPQDVFI NSELKLEEID VFGFDYDYTL ANYGDQVQHL
     IYDLAMSHLV DEQKYPMALK EIKYDPTFAI RGLHFDVNHG LLMKLDYLNN IQAGAIYHGR
     RPLTKEEVIQ IYGSMQLKRL YCDSFLKPMS DIFCLPEACL IANTIQYLTD HNLAFEPRII
     HEDVTAAVGK VHLGGGLHNK IISDFPLYLN KHPLLGEFLL KLKSHGKKLF LLTNNSYFYA
     NHGMKYLLND QLNGKYEDWT DVFDVIITKC DKPSFFGKGR PFRMYHPDSD RYDWNEVNHF
     EPKKVYVGGS LKQFTNVSKW RGRSVMYFGD HLYSDLVEPS QKEGWKTGVI IKELEVEVGI
     QNSPKYREQL AELLQLEDVI RKCQFFSGEK KELFLEQLKT ERYKKRLALK EPFNANFGSL
     FRTHTNATIF ASSLQRHADI YTSKIENLIS YPLTASLIPS RNYLPHEFKL N