ID   NTAQ1_CAEEL             Reviewed;         184 AA.
AC   Q21775;
DT   01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Protein N-terminal glutamine amidohydrolase;
DE            EC=3.5.1.122 {ECO:0000250|UniProtKB:Q80WB5};
DE   AltName: Full=Protein NH2-terminal glutamine deamidase;
DE            Short=N-terminal Gln amidase;
DE            Short=Nt(Q)-amidase;
GN   ORFNames=R06C7.6;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
CC   -!- FUNCTION: Mediates the side-chain deamidation of N-terminal glutamine
CC       residues to glutamate, an important step in N-end rule pathway of
CC       protein degradation. Conversion of the resulting N-terminal glutamine
CC       to glutamate renders the protein susceptible to arginylation,
CC       polyubiquitination and degradation as specified by the N-end rule. Does
CC       not act on substrates with internal or C-terminal glutamine and does
CC       not act on non-glutamine residues in any position.
CC       {ECO:0000250|UniProtKB:Q80WB5}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + N-terminal L-glutaminyl-[protein] = N-terminal L-
CC         glutamyl-[protein] + NH4(+); Xref=Rhea:RHEA:50680, Rhea:RHEA-
CC         COMP:12668, Rhea:RHEA-COMP:12777, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:28938, ChEBI:CHEBI:64721, ChEBI:CHEBI:64722;
CC         EC=3.5.1.122; Evidence={ECO:0000250|UniProtKB:Q80WB5};
CC   -!- SUBUNIT: Monomer. {ECO:0000250|UniProtKB:Q96HA8}.
CC   -!- SIMILARITY: Belongs to the NTAQ1 family. {ECO:0000305}.
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DR   EMBL; Z71266; CAA95844.1; -; Genomic_DNA.
DR   PIR; T23970; T23970.
DR   RefSeq; NP_492048.1; NM_059647.1.
DR   AlphaFoldDB; Q21775; -.
DR   SMR; Q21775; -.
DR   STRING; 6239.R06C7.6; -.
DR   PaxDb; Q21775; -.
DR   EnsemblMetazoa; R06C7.6.1; R06C7.6.1; WBGene00011065.
DR   GeneID; 187641; -.
DR   KEGG; cel:CELE_R06C7.6; -.
DR   UCSC; R06C7.6; c. elegans.
DR   CTD; 187641; -.
DR   WormBase; R06C7.6; CE06249; WBGene00011065; -.
DR   eggNOG; KOG3261; Eukaryota.
DR   GeneTree; ENSGT00390000014398; -.
DR   HOGENOM; CLU_091083_1_0_1; -.
DR   InParanoid; Q21775; -.
DR   OMA; GWGTVYS; -.
DR   OrthoDB; 1337506at2759; -.
DR   PhylomeDB; Q21775; -.
DR   PRO; PR:Q21775; -.
DR   Proteomes; UP000001940; Chromosome I.
DR   Bgee; WBGene00011065; Expressed in germ line (C elegans) and 4 other tissues.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0008418; F:protein-N-terminal asparagine amidohydrolase activity; IEA:InterPro.
DR   GO; GO:0070773; F:protein-N-terminal glutamine amidohydrolase activity; IBA:GO_Central.
DR   GO; GO:0036211; P:protein modification process; IBA:GO_Central.
DR   Gene3D; 3.10.620.10; -; 1.
DR   InterPro; IPR037132; N_Gln_amidohydro_ab_roll_sf.
DR   InterPro; IPR039733; NTAQ1.
DR   InterPro; IPR023128; Prot_N_Gln_amidohydro_ab_roll.
DR   PANTHER; PTHR13035; PTHR13035; 1.
DR   Pfam; PF09764; Nt_Gln_amidase; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Reference proteome.
FT   CHAIN           1..184
FT                   /note="Protein N-terminal glutamine amidohydrolase"
FT                   /id="PRO_0000381821"
FT   ACT_SITE        14
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        63
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        78
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   184 AA;  21593 MW;  9265C2E74C1DBAA1 CRC64;
     MISAEEALYQ SCYCEENVYK LIEKIDDKNG IFAIIISNDC KMIPLWKQKA AKSINGHVLW
     DYHVIAIEKE KNGSKVYDLD STLEWSCDFL EYWEKTMNLA EMAQRDTKFR RKFRVIPGEN
     YISLLSSDRS HMLSKNGVYL KPPPEWPLIN SHKPSNLMEL IRMSEQIENT TVMDEPELFQ
     LFSK