ID   NTA_MACGS               Reviewed;          81 AA.
AC   Q75WH5;
DT   01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 42.
DE   RecName: Full=U9-hexatoxin-Mg1a;
DE            Short=U9-HXTX-Mg1a;
DE   AltName: Full=Neurotoxin magi-8 {ECO:0000303|PubMed:15246762};
DE   Flags: Precursor;
OS   Macrothele gigas (Japanese funnel web spider).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Mygalomorphae; Macrothelidae; Macrothele.
OX   NCBI_TaxID=223896;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AMIDATION AT PRO-79, AND MASS
RP   SPECTROMETRY.
RC   TISSUE=Venom, and Venom gland;
RX   PubMed=15246762; DOI=10.1016/j.toxicon.2004.05.012;
RA   Satake H., Villegas E., Oshiro N., Terada K., Shinada T., Corzo G.;
RT   "Rapid and efficient identification of cysteine-rich peptides by random
RT   screening of a venom gland cDNA library from the hexathelid spider
RT   Macrothele gigas.";
RL   Toxicon 44:149-156(2004).
CC   -!- FUNCTION: No toxicity is observed upon intracranial injection into mice
CC       and intrathorax injection into crickets. {ECO:0000269|PubMed:15246762}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000250}.
CC   -!- MASS SPECTROMETRY: Mass=3694.2; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:15246762};
CC   -!- SIMILARITY: Belongs to the neurotoxin 10 (Hwtx-1) family.
CC       {ECO:0000305}.
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DR   EMBL; AB121196; BAD13403.1; -; mRNA.
DR   AlphaFoldDB; Q75WH5; -.
DR   SMR; Q75WH5; -.
DR   ArachnoServer; AS000388; U9-hexatoxin-Mg1a.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   InterPro; IPR011696; Huwentoxin-1.
DR   Pfam; PF07740; Toxin_12; 1.
PE   1: Evidence at protein level;
KW   Amidation; Disulfide bond; Knottin; Secreted; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   PROPEP          20..45
FT                   /id="PRO_0000285703"
FT   CHAIN           46..79
FT                   /note="U9-hexatoxin-Mg1a"
FT                   /id="PRO_0000285704"
FT   MOD_RES         79
FT                   /note="Proline amide"
FT                   /evidence="ECO:0000269|PubMed:15246762"
FT   DISULFID        47..61
FT                   /evidence="ECO:0000250"
FT   DISULFID        54..66
FT                   /evidence="ECO:0000250"
FT   DISULFID        60..74
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   81 AA;  9043 MW;  534E7162AC41ECFB CRC64;
     MKYVMVILGL LVLAAICCAS EVEELDWRQE IARAILEAEL QPEERDCKGL FRQCKKSSEC
     CKGSSCESDL TGLCIFNLPG R