ID   NTA_ORPS2               Reviewed;          33 AA.
AC   P0DP95;
DT   25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2017, sequence version 1.
DT   25-MAY-2022, entry version 10.
DE   RecName: Full=Mu-theraphotoxin-Osp1a {ECO:0000303|PubMed:25754331};
DE            Short=Mu-TRTX-Osp1a {ECO:0000303|PubMed:25754331};
OS   Orphnaecus sp. (strain Sibaliw/Philippines) (Tarantula spider).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Mygalomorphae; Theraphosidae; Orphnaecus; unclassified Orphnaecus.
OX   NCBI_TaxID=2024663;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RC   TISSUE=Venom;
RX   PubMed=25754331; DOI=10.1111/bph.13081;
RA   Klint J.K., Smith J.J., Vetter I., Rupasinghe D.B., Er S.Y., Senff S.,
RA   Herzig V., Mobli M., Lewis R.J., Bosmans F., King G.F.;
RT   "Seven novel modulators of the analgesic target NaV 1.7 uncovered using a
RT   high-throughput venom-based discovery approach.";
RL   Br. J. Pharmacol. 172:2445-2458(2015).
CC   -!- FUNCTION: Voltage-gated sodium channel Nav1.7/SCN9A inhibitor.
CC       {ECO:0000269|PubMed:25754331}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:25754331}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:25754331}.
CC   -!- MASS SPECTROMETRY: Mass=3692.5; Method=MALDI; Note=Monoisotopic mass.;
CC       Evidence={ECO:0000269|PubMed:25754331};
CC   -!- SIMILARITY: Belongs to the neurotoxin 10 (Hwtx-1) family.
CC       {ECO:0000305}.
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DR   AlphaFoldDB; P0DP95; -.
DR   SMR; P0DP95; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0017080; F:sodium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR011696; Huwentoxin-1.
DR   InterPro; IPR013140; Huwentoxin_CS1.
DR   Pfam; PF07740; Toxin_12; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW   Knottin; Secreted; Toxin; Voltage-gated sodium channel impairing toxin.
FT   CHAIN           1..33
FT                   /note="Mu-theraphotoxin-Osp1a"
FT                   /evidence="ECO:0000269|PubMed:25754331"
FT                   /id="PRO_0000441853"
FT   DISULFID        2..17
FT                   /evidence="ECO:0000250|UniProtKB:D2Y2D1"
FT   DISULFID        9..22
FT                   /evidence="ECO:0000250|UniProtKB:D2Y2D1"
FT   DISULFID        16..29
FT                   /evidence="ECO:0000250|UniProtKB:D2Y2D1"
SQ   SEQUENCE   33 AA;  3701 MW;  20D51AE348D7861A CRC64;
     GCKGFGKACK YGADECCKNL VCSKKHKWCK YTL