NTC20_YEAST
ID NTC20_YEAST Reviewed; 140 AA.
AC P38302; D6VQI3;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Pre-mRNA-splicing factor NTC20;
DE AltName: Full=PRP19-associated complex protein 20;
DE AltName: Full=Synergistic to PRP19 mutation protein 384;
GN Name=NTC20; Synonyms=SNT384; OrderedLocusNames=YBR188C; ORFNames=YBR1316;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7871891; DOI=10.1002/yea.320101116;
RA Demolis N., Jacquet M., Mallet L.;
RT "A 12.5 kb fragment of the yeast chromosome II contains two adjacent genes
RT encoding ribosomal proteins and six putative new genes, one of which
RT encodes a putative transcriptional factor.";
RL Yeast 10:1511-1525(1994).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA Mewes H.-W., Kleine K.;
RT "Complete DNA sequence of yeast chromosome II.";
RL EMBO J. 13:5795-5809(1994).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP FUNCTION, AND INTERACTION WITH CEF1; CLF1 AND SYF1.
RX PubMed=11102353; DOI=10.1093/genetics/156.4.1503;
RA Ben-Yehuda S., Dix I., Russell C.S., McGarvey M., Beggs J.D., Kupiec M.;
RT "Genetic and physical interactions between factors involved in both cell
RT cycle progression and pre-mRNA splicing in Saccharomyces cerevisiae.";
RL Genetics 156:1503-1517(2000).
RN [6]
RP FUNCTION, INTERACTION WITH CEF1, AND IDENTIFICATION IN THE PRP19-ASSOCIATED
RP COMPLEX.
RX PubMed=11018040; DOI=10.1074/jbc.m006958200;
RA Chen C.-H., Tsai W.-Y., Chen H.-R., Wang C.-H., Cheng S.-C.;
RT "Identification and characterization of two novel components of the Prp19p-
RT associated complex, Ntc30p and Ntc20p.";
RL J. Biol. Chem. 276:488-494(2001).
RN [7]
RP IDENTIFICATION IN THE CWC COMPLEX, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=11884590; DOI=10.1128/mcb.22.7.2011-2024.2002;
RA Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.;
RT "Proteomics analysis reveals stable multiprotein complexes in both fission
RT and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA
RT splicing factors, and snRNAs.";
RL Mol. Cell. Biol. 22:2011-2024(2002).
RN [8]
RP FUNCTION, AND IDENTIFICATION IN THE PRP19-ASSOCIATED COMPLEX.
RX PubMed=11842115; DOI=10.1093/nar/30.4.1029;
RA Chen C.-H., Yu W.-C., Tsao T.Y., Wang L.-Y., Chen H.-R., Lin J.-Y.,
RA Tsai W.-Y., Cheng S.-C.;
RT "Functional and physical interactions between components of the Prp19p-
RT associated complex.";
RL Nucleic Acids Res. 30:1029-1037(2002).
RN [9]
RP INTERACTION WITH CLF1; PRP46 AND SYF1.
RX PubMed=12088152; DOI=10.1017/s1355838202025050;
RA Ohi M.D., Gould K.L.;
RT "Characterization of interactions among the Cef1p-Prp19p-associated
RT splicing complex.";
RL RNA 8:798-815(2002).
RN [10]
RP FUNCTION.
RX PubMed=12871902; DOI=10.1093/genetics/164.3.895;
RA Vincent K., Wang Q., Jay S., Hobbs K., Rymond B.C.;
RT "Genetic interactions with CLF1 identify additional pre-mRNA splicing
RT factors and a link between activators of yeast vesicular transport and
RT splicing.";
RL Genetics 164:895-907(2003).
RN [11]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [12]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [13]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-139, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [14]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-139, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: Involved in pre-mRNA splicing. As a component of the NTC
CC complex, associates to the spliceosome to mediate conformational
CC rearrangement or to stabilize the structure of the spliceosome after U4
CC snRNA dissociation, which leads to spliceosome maturation.
CC {ECO:0000269|PubMed:11018040, ECO:0000269|PubMed:11102353,
CC ECO:0000269|PubMed:11842115, ECO:0000269|PubMed:12871902}.
CC -!- SUBUNIT: Belongs to the NTC complex (or PRP19-associated complex),
CC composed of at least CEF1, CLF1, ISY1, NTC20, SNT309, SYF1, SYF2, and
CC PRP19. The NTC complex associates with the spliceosome after the
CC release of the U1 and U4 snRNAs and forms the CWC spliceosome
CC subcomplex (or CEF1-associated complex) reminiscent of a late-stage
CC spliceosome composed also of the U2, U5 and U6 snRNAs and at least
CC BUD13, BRR2, CDC40, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24,
CC CWC25, CWC27, ECM2, HSH155, IST3, LEA1, MSL1, PRP8, PRP9, PRP11, PRP21,
CC PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNU114,
CC SPP2, RSE1 and YJU2. Interacts with CEF1, CLF1, ISY1, PRP46, and SYF1.
CC {ECO:0000269|PubMed:11018040, ECO:0000269|PubMed:11102353,
CC ECO:0000269|PubMed:11842115, ECO:0000269|PubMed:11884590,
CC ECO:0000269|PubMed:12088152}.
CC -!- INTERACTION:
CC P38302; Q03654: CEF1; NbExp=4; IntAct=EBI-20921, EBI-476;
CC P38302; Q12309: CLF1; NbExp=6; IntAct=EBI-20921, EBI-484;
CC P38302; P32523: PRP19; NbExp=5; IntAct=EBI-20921, EBI-493;
CC P38302; Q06091: SNT309; NbExp=2; IntAct=EBI-20921, EBI-818;
CC P38302; Q04048: SYF1; NbExp=6; IntAct=EBI-20921, EBI-540;
CC P38302; P53277: SYF2; NbExp=2; IntAct=EBI-20921, EBI-23308;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 1430 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; U02073; AAB60285.1; -; Genomic_DNA.
DR EMBL; Z36057; CAA85149.1; -; Genomic_DNA.
DR EMBL; AY558127; AAS56453.1; -; Genomic_DNA.
DR EMBL; BK006936; DAA07303.1; -; Genomic_DNA.
DR PIR; S46060; S46060.
DR RefSeq; NP_009747.3; NM_001178536.3.
DR AlphaFoldDB; P38302; -.
DR BioGRID; 32886; 80.
DR ComplexPortal; CPX-1651; PRP19-associated complex.
DR ComplexPortal; CPX-1885; NineTeen complex.
DR DIP; DIP-1683N; -.
DR IntAct; P38302; 15.
DR MINT; P38302; -.
DR STRING; 4932.YBR188C; -.
DR iPTMnet; P38302; -.
DR MaxQB; P38302; -.
DR PaxDb; P38302; -.
DR PRIDE; P38302; -.
DR TopDownProteomics; P38302; -.
DR EnsemblFungi; YBR188C_mRNA; YBR188C; YBR188C.
DR GeneID; 852486; -.
DR KEGG; sce:YBR188C; -.
DR SGD; S000000392; NTC20.
DR VEuPathDB; FungiDB:YBR188C; -.
DR HOGENOM; CLU_1836689_0_0_1; -.
DR InParanoid; P38302; -.
DR OMA; PQKNEHQ; -.
DR BioCyc; YEAST:G3O-29131-MON; -.
DR PRO; PR:P38302; -.
DR Proteomes; UP000002311; Chromosome II.
DR RNAct; P38302; protein.
DR GO; GO:0000974; C:Prp19 complex; IDA:SGD.
DR GO; GO:0071006; C:U2-type catalytic step 1 spliceosome; IDA:SGD.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IGI:SGD.
PE 1: Evidence at protein level;
KW mRNA processing; mRNA splicing; Nucleus; Phosphoprotein;
KW Reference proteome; Spliceosome.
FT CHAIN 1..140
FT /note="Pre-mRNA-splicing factor NTC20"
FT /id="PRO_0000057974"
FT MOD_RES 139
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956,
FT ECO:0007744|PubMed:19779198"
SQ SEQUENCE 140 AA; 15970 MW; 15C5234B25213223 CRC64;
MPSLRDLSLE RDQELNQLRA RINQLGKTGK EEANDFVGLN ISNEPVYDTV IQTGQSSNAT
NSFVQETIQK TKQKESGQPY IIPQKNEHQR YIDKVCETSD LKAKLAPIME VLEKKTNEKI
KGIIRKRVLQ EPDRDNDDSG