NTCA_NOSS1
ID NTCA_NOSS1 Reviewed; 223 AA.
AC P0A4U6; Q05061;
DT 15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2005, sequence version 1.
DT 25-MAY-2022, entry version 93.
DE RecName: Full=Global nitrogen regulator;
DE AltName: Full=DNA-binding protein VF1;
DE AltName: Full=Nitrogen-responsive regulatory protein;
GN Name=ntcA; Synonyms=bifA; OrderedLocusNames=alr4392;
OS Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576).
OC Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Nostoc.
OX NCBI_TaxID=103690;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8391534; DOI=10.1128/jb.175.13.4025-4035.1993;
RA Wei T.-F., Ramasubramanian T.S., Pu F., Golden J.W.;
RT "Anabaena sp. strain PCC 7120 bifA gene encoding a sequence-specific DNA-
RT binding protein cloned by in vivo transcriptional interference selection.";
RL J. Bacteriol. 175:4025-4035(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8366058; DOI=10.1128/jb.175.17.5710-5713.1993;
RA Frias J.E., Merida A., Herrero A., Martin-Nieto J.M., Flores E.;
RT "General distribution of the nitrogen control gene ntcA in cyanobacteria.";
RL J. Bacteriol. 175:5710-5713(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 7120 / SAG 25.82 / UTEX 2576;
RX PubMed=11759840; DOI=10.1093/dnares/8.5.205;
RA Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A.,
RA Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M.,
RA Takazawa M., Yamada M., Yasuda M., Tabata S.;
RT "Complete genomic sequence of the filamentous nitrogen-fixing
RT cyanobacterium Anabaena sp. strain PCC 7120.";
RL DNA Res. 8:205-213(2001).
CC -!- FUNCTION: Required for full expression of proteins subject to ammonium
CC repression. Transcriptional activator of genes subject to nitrogen
CC control.
CC -!- FUNCTION: Has affinity for the xisA upstream region. Binds to a 66 bp
CC region containing three repeats of the consensus recognition sequence
CC 5'-ACATT-3'.
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DR EMBL; L10036; AAD04183.1; -; Genomic_DNA.
DR EMBL; X71608; CAA50608.1; -; Genomic_DNA.
DR EMBL; BA000019; BAB76091.1; -; Genomic_DNA.
DR PIR; AH2354; AH2354.
DR PIR; B48640; B48640.
DR RefSeq; WP_010998529.1; NZ_RSCN01000051.1.
DR PDB; 3LA2; X-ray; 2.60 A; A/B=1-223.
DR PDB; 3LA3; X-ray; 2.40 A; A/B=1-223.
DR PDB; 3LA7; X-ray; 1.90 A; A/B=1-223.
DR PDBsum; 3LA2; -.
DR PDBsum; 3LA3; -.
DR PDBsum; 3LA7; -.
DR AlphaFoldDB; P0A4U6; -.
DR SMR; P0A4U6; -.
DR DIP; DIP-59350N; -.
DR STRING; 103690.17133528; -.
DR EnsemblBacteria; BAB76091; BAB76091; BAB76091.
DR GeneID; 58726067; -.
DR KEGG; ana:alr4392; -.
DR eggNOG; COG0664; Bacteria.
DR OMA; VLCRDFG; -.
DR OrthoDB; 1596937at2; -.
DR EvolutionaryTrace; P0A4U6; -.
DR Proteomes; UP000002483; Chromosome.
DR CollecTF; EXPREG_00000760; -.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR CDD; cd00038; CAP_ED; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 2.60.120.10; -; 1.
DR InterPro; IPR018490; cNMP-bd-like.
DR InterPro; IPR000595; cNMP-bd_dom.
DR InterPro; IPR012318; HTH_CRP.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR018335; Tscrpt_reg_HTH_Crp-type_CS.
DR InterPro; IPR022299; Tscrpt_reg_NtcA.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00027; cNMP_binding; 1.
DR Pfam; PF13545; HTH_Crp_2; 1.
DR PRINTS; PR00034; HTHCRP.
DR SMART; SM00100; cNMP; 1.
DR SMART; SM00419; HTH_CRP; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF51206; SSF51206; 1.
DR TIGRFAMs; TIGR03697; NtcA_cyano; 1.
DR PROSITE; PS50042; CNMP_BINDING_3; 1.
DR PROSITE; PS00042; HTH_CRP_1; 1.
DR PROSITE; PS51063; HTH_CRP_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; DNA-binding; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..223
FT /note="Global nitrogen regulator"
FT /id="PRO_0000100184"
FT DOMAIN 143..216
FT /note="HTH crp-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00387"
FT DNA_BIND 176..195
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00387"
FT HELIX 7..16
FT /evidence="ECO:0007829|PDB:3LA7"
FT STRAND 24..28
FT /evidence="ECO:0007829|PDB:3LA7"
FT STRAND 33..35
FT /evidence="ECO:0007829|PDB:3LA7"
FT STRAND 43..50
FT /evidence="ECO:0007829|PDB:3LA7"
FT STRAND 52..57
FT /evidence="ECO:0007829|PDB:3LA7"
FT STRAND 63..69
FT /evidence="ECO:0007829|PDB:3LA7"
FT STRAND 74..76
FT /evidence="ECO:0007829|PDB:3LA7"
FT HELIX 78..81
FT /evidence="ECO:0007829|PDB:3LA7"
FT HELIX 83..85
FT /evidence="ECO:0007829|PDB:3LA3"
FT STRAND 90..104
FT /evidence="ECO:0007829|PDB:3LA7"
FT HELIX 105..112
FT /evidence="ECO:0007829|PDB:3LA7"
FT HELIX 116..141
FT /evidence="ECO:0007829|PDB:3LA7"
FT HELIX 145..160
FT /evidence="ECO:0007829|PDB:3LA7"
FT STRAND 161..163
FT /evidence="ECO:0007829|PDB:3LA7"
FT STRAND 165..170
FT /evidence="ECO:0007829|PDB:3LA7"
FT HELIX 176..183
FT /evidence="ECO:0007829|PDB:3LA7"
FT HELIX 187..199
FT /evidence="ECO:0007829|PDB:3LA7"
FT STRAND 202..206
FT /evidence="ECO:0007829|PDB:3LA7"
FT STRAND 209..212
FT /evidence="ECO:0007829|PDB:3LA7"
FT HELIX 215..218
FT /evidence="ECO:0007829|PDB:3LA3"
FT TURN 219..221
FT /evidence="ECO:0007829|PDB:3LA3"
SQ SEQUENCE 223 AA; 24918 MW; 07FCE891766E7021 CRC64;
MIVTQDKALA NVFRQMATGA FPPVVETFER NKTIFFPGDP AERVYFLLKG AVKLSRVYEA
GEEITVALLR ENSVFGVLSL LTGNKSDRFY HAVAFTPVEL LSAPIEQVEQ ALKENPELSM
LMLRGLSSRI LQTEMMIETL AHRDMGSRLV SFLLILCRDF GVPCADGITI DLKLSHQAIA
EAIGSTRVTV TRLLGDLREK KMISIHKKKI TVHKPVTLSR QFT
