NTCA_SYNE7
ID NTCA_SYNE7 Reviewed; 222 AA.
AC P29283; Q31S10;
DT 01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-1992, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Global nitrogen regulator;
GN Name=ntcA; OrderedLocusNames=Synpcc7942_0127;
OS Synechococcus elongatus (strain PCC 7942 / FACHB-805) (Anacystis nidulans
OS R2).
OC Bacteria; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus.
OX NCBI_TaxID=1140;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1630321; DOI=10.1111/j.1365-2958.1992.tb01357.x;
RA Vega-Palas M.A., Flores E., Herrero A.;
RT "NtcA, a global nitrogen regulator from the cyanobacterium Synechococcus
RT that belongs to the Crp family of bacterial regulators.";
RL Mol. Microbiol. 6:1853-1859(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 7942 / FACHB-805;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M.,
RA Kyrpides N., Lykidis A., Richardson P.;
RT "Complete sequence of chromosome 1 of Synechococcus elongatus PCC 7942.";
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Required for full expression of proteins subject to ammonium
CC repression. Transcriptional activator of genes subject to nitrogen
CC control.
CC -!- INTERACTION:
CC P29283; Q7X386: Synpcc7942_2061; NbExp=3; IntAct=EBI-15870797, EBI-700982;
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DR EMBL; X60197; CAA42755.1; -; Genomic_DNA.
DR EMBL; CP000100; ABB56159.1; -; Genomic_DNA.
DR PIR; S25244; S25244.
DR PDB; 2XGX; X-ray; 2.85 A; A/B=1-222.
DR PDB; 2XHK; X-ray; 2.30 A; A/B=1-222.
DR PDB; 2XKO; X-ray; 2.25 A; A/B=1-222.
DR PDB; 2XKP; X-ray; 3.05 A; A/B/C/D/E/F=1-222.
DR PDBsum; 2XGX; -.
DR PDBsum; 2XHK; -.
DR PDBsum; 2XKO; -.
DR PDBsum; 2XKP; -.
DR AlphaFoldDB; P29283; -.
DR SMR; P29283; -.
DR DIP; DIP-59550N; -.
DR IntAct; P29283; 1.
DR STRING; 1140.Synpcc7942_0127; -.
DR PRIDE; P29283; -.
DR EnsemblBacteria; ABB56159; ABB56159; Synpcc7942_0127.
DR KEGG; syf:Synpcc7942_0127; -.
DR eggNOG; COG0664; Bacteria.
DR HOGENOM; CLU_075053_3_2_3; -.
DR OMA; VLCRDFG; -.
DR BioCyc; SYNEL:NTCA; -.
DR EvolutionaryTrace; P29283; -.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR CDD; cd00038; CAP_ED; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 2.60.120.10; -; 1.
DR InterPro; IPR018490; cNMP-bd-like.
DR InterPro; IPR000595; cNMP-bd_dom.
DR InterPro; IPR012318; HTH_CRP.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR018335; Tscrpt_reg_HTH_Crp-type_CS.
DR InterPro; IPR022299; Tscrpt_reg_NtcA.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00027; cNMP_binding; 1.
DR Pfam; PF13545; HTH_Crp_2; 1.
DR PRINTS; PR00034; HTHCRP.
DR SMART; SM00100; cNMP; 1.
DR SMART; SM00419; HTH_CRP; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF51206; SSF51206; 1.
DR TIGRFAMs; TIGR03697; NtcA_cyano; 1.
DR PROSITE; PS50042; CNMP_BINDING_3; 1.
DR PROSITE; PS00042; HTH_CRP_1; 1.
DR PROSITE; PS51063; HTH_CRP_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; DNA-binding; Transcription;
KW Transcription regulation.
FT CHAIN 1..222
FT /note="Global nitrogen regulator"
FT /id="PRO_0000100186"
FT DOMAIN 142..215
FT /note="HTH crp-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00387"
FT DNA_BIND 175..194
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00387"
FT BINDING 6..128
FT /ligand="a nucleoside 3',5'-cyclic phosphate"
FT /ligand_id="ChEBI:CHEBI:58464"
FT HELIX 12..15
FT /evidence="ECO:0007829|PDB:2XKO"
FT STRAND 18..20
FT /evidence="ECO:0007829|PDB:2XKO"
FT STRAND 23..27
FT /evidence="ECO:0007829|PDB:2XKO"
FT STRAND 32..34
FT /evidence="ECO:0007829|PDB:2XKO"
FT STRAND 42..49
FT /evidence="ECO:0007829|PDB:2XKO"
FT STRAND 51..56
FT /evidence="ECO:0007829|PDB:2XKO"
FT STRAND 58..60
FT /evidence="ECO:0007829|PDB:2XKP"
FT STRAND 62..68
FT /evidence="ECO:0007829|PDB:2XKO"
FT STRAND 72..74
FT /evidence="ECO:0007829|PDB:2XKP"
FT HELIX 78..81
FT /evidence="ECO:0007829|PDB:2XKO"
FT HELIX 82..84
FT /evidence="ECO:0007829|PDB:2XKO"
FT STRAND 89..95
FT /evidence="ECO:0007829|PDB:2XKO"
FT STRAND 97..103
FT /evidence="ECO:0007829|PDB:2XKO"
FT HELIX 104..106
FT /evidence="ECO:0007829|PDB:2XKO"
FT HELIX 107..113
FT /evidence="ECO:0007829|PDB:2XKO"
FT HELIX 116..140
FT /evidence="ECO:0007829|PDB:2XKO"
FT HELIX 144..159
FT /evidence="ECO:0007829|PDB:2XKO"
FT STRAND 160..163
FT /evidence="ECO:0007829|PDB:2XKO"
FT STRAND 166..171
FT /evidence="ECO:0007829|PDB:2XKO"
FT HELIX 175..182
FT /evidence="ECO:0007829|PDB:2XKO"
FT HELIX 186..198
FT /evidence="ECO:0007829|PDB:2XKO"
FT STRAND 201..205
FT /evidence="ECO:0007829|PDB:2XKO"
FT STRAND 208..212
FT /evidence="ECO:0007829|PDB:2XKO"
FT HELIX 214..219
FT /evidence="ECO:0007829|PDB:2XKO"
SQ SEQUENCE 222 AA; 24833 MW; C6A9DC575D15A69A CRC64;
MLANENSLLT MFRELGSGKL PLQIEQFERG KTIFFPGDPA ERVYLLVKGA VKLSRVYESG
EEITVALLRE NSVFGVLSLL TGQRSDRFYH AVAFTPVQLF SVPIEFMQKA LIERPELANV
MLQGLSSRIL QTEMMIETLA HRDMGSRLVS FLLILCRDFG IPSPDGITID LKLSHQAIAE
AIGSTRVTVT RLLGDLRESK LIAIHKKRIT VFNPVALSQQ FS
