NTCP2_MOUSE
ID NTCP2_MOUSE Reviewed; 348 AA.
AC P70172; Q8VI83; Q925U7;
DT 26-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 2.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Ileal sodium/bile acid cotransporter;
DE AltName: Full=Apical sodium-dependent bile acid transporter;
DE Short=ASBT;
DE AltName: Full=Ileal Na(+)/bile acid cotransporter;
DE AltName: Full=Ileal sodium-dependent bile acid transporter;
DE Short=IBAT;
DE Short=ISBT;
DE AltName: Full=Na(+)-dependent ileal bile acid transporter;
DE AltName: Full=Sodium/taurocholate cotransporting polypeptide, ileal;
DE AltName: Full=Solute carrier family 10 member 2;
GN Name=Slc10a2; Synonyms=Ntcp2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=ICR;
RX PubMed=10101301; DOI=10.1093/oxfordjournals.jbchem.a022358;
RA Saeki T., Matoba K., Furukawa H., Kirifuji K., Kanamoto R., Iwami K.;
RT "Characterization, cDNA cloning, and functional expression of mouse ileal
RT sodium-dependent bile acid transporter.";
RL J. Biochem. 125:846-851(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE, AND VARIANTS VAL-29; SER-36; THR-40 AND ILE-178.
RC STRAIN=C57BL/6J;
RA Dawson P.A., Craddock A.L., Tietjen M.E., Haywood J.H.;
RT "Disruption of the ileal bile acid transporter gene in mice.";
RL Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE, AND VARIANTS VAL-29; SER-36; THR-40 AND ILE-178.
RC STRAIN=NZB/BlNJ, and SM/J;
RA Lyons M.A., Korstanje R., Li R., Sheehan S.M., Walsh K.A., Carey M.C.,
RA Churchill G.A., Paigen B.;
RT "New genetic determinants of cholesterol gallstone formation revealed in an
RT intercross of mouse strains NZB and SM.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE OF 1-125.
RA Saeki T., Kirifuji K., Kanamoto R., Iwami K.;
RT "Identification of transcription start sites in mouse ileal sodium-
RT dependent bile acid transporter gene.";
RL Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-335, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Plays a critical role in the sodium-dependent reabsorption of
CC bile acids from the lumen of the small intestine. Plays a key role in
CC cholesterol metabolism (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Monomer and homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the bile acid:sodium symporter (BASS) (TC
CC 2.A.28) family. {ECO:0000305}.
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DR EMBL; AB002693; BAA19606.1; -; mRNA.
DR EMBL; D87059; BAA13237.1; -; mRNA.
DR EMBL; AF271073; AAK55514.1; -; Genomic_DNA.
DR EMBL; AF266724; AAK55514.1; JOINED; Genomic_DNA.
DR EMBL; AF266725; AAK55514.1; JOINED; Genomic_DNA.
DR EMBL; AF266726; AAK55514.1; JOINED; Genomic_DNA.
DR EMBL; AF266727; AAK55514.1; JOINED; Genomic_DNA.
DR EMBL; AF266728; AAK55514.1; JOINED; Genomic_DNA.
DR EMBL; AY529655; AAS20611.1; -; mRNA.
DR EMBL; AB078635; BAB84081.1; -; Genomic_DNA.
DR CCDS; CCDS22089.1; -.
DR RefSeq; NP_035518.1; NM_011388.3.
DR AlphaFoldDB; P70172; -.
DR SMR; P70172; -.
DR BioGRID; 203275; 1.
DR STRING; 10090.ENSMUSP00000023835; -.
DR BindingDB; P70172; -.
DR ChEMBL; CHEMBL2073708; -.
DR GlyGen; P70172; 2 sites.
DR iPTMnet; P70172; -.
DR PhosphoSitePlus; P70172; -.
DR jPOST; P70172; -.
DR PaxDb; P70172; -.
DR PRIDE; P70172; -.
DR ProteomicsDB; 287831; -.
DR Antibodypedia; 11241; 204 antibodies from 31 providers.
DR DNASU; 20494; -.
DR Ensembl; ENSMUST00000023835; ENSMUSP00000023835; ENSMUSG00000023073.
DR GeneID; 20494; -.
DR KEGG; mmu:20494; -.
DR UCSC; uc009kub.1; mouse.
DR CTD; 6555; -.
DR MGI; MGI:1201406; Slc10a2.
DR VEuPathDB; HostDB:ENSMUSG00000023073; -.
DR eggNOG; KOG2718; Eukaryota.
DR GeneTree; ENSGT00950000182808; -.
DR HOGENOM; CLU_034788_7_5_1; -.
DR InParanoid; P70172; -.
DR OMA; QFTIMPL; -.
DR OrthoDB; 1148347at2759; -.
DR PhylomeDB; P70172; -.
DR TreeFam; TF315811; -.
DR Reactome; R-MMU-159418; Recycling of bile acids and salts.
DR BioGRID-ORCS; 20494; 1 hit in 75 CRISPR screens.
DR PRO; PR:P70172; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; P70172; protein.
DR Bgee; ENSMUSG00000023073; Expressed in proximal tubule and 35 other tissues.
DR ExpressionAtlas; P70172; baseline and differential.
DR Genevisible; P70172; MM.
DR GO; GO:0016324; C:apical plasma membrane; IDA:MGI.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005902; C:microvillus; IDA:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0000502; C:proteasome complex; ISO:MGI.
DR GO; GO:0008508; F:bile acid:sodium symporter activity; ISO:MGI.
DR GO; GO:0015721; P:bile acid and bile salt transport; ISO:MGI.
DR GO; GO:0009617; P:response to bacterium; IEP:MGI.
DR Gene3D; 1.20.1530.20; -; 1.
DR InterPro; IPR002657; BilAc:Na_symport/Acr3.
DR InterPro; IPR004710; Bilac:Na_transpt.
DR InterPro; IPR038770; Na+/solute_symporter_sf.
DR InterPro; IPR030207; SLC10A2.
DR PANTHER; PTHR10361; PTHR10361; 1.
DR PANTHER; PTHR10361:SF19; PTHR10361:SF19; 1.
DR Pfam; PF01758; SBF; 1.
DR TIGRFAMs; TIGR00841; bass; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Ion transport; Membrane; Phosphoprotein; Reference proteome;
KW Sodium; Sodium transport; Symport; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..348
FT /note="Ileal sodium/bile acid cotransporter"
FT /id="PRO_0000052340"
FT TOPO_DOM 1..28
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 29..49
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 50..87
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 88..108
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 109..126
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 127..147
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 148..157
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 158..178
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 179..195
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 196..216
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 217..224
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 225..245
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 246..284
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 285..305
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 306..348
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 328..348
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 335
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CARBOHYD 3
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 10
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VARIANT 29
FT /note="I -> V (in strain: NZB/BlNJ and C57BL/6)"
FT /evidence="ECO:0000269|Ref.2, ECO:0000269|Ref.3"
FT VARIANT 36
FT /note="T -> S (in strain: NZB/BlNJ and C57BL/6)"
FT /evidence="ECO:0000269|Ref.2, ECO:0000269|Ref.3"
FT VARIANT 40
FT /note="I -> T (in strain: NZB/BlNJ and C57BL/6)"
FT /evidence="ECO:0000269|Ref.2, ECO:0000269|Ref.3"
FT VARIANT 178
FT /note="F -> I (in strain: NZB/BlNJ and C57BL/6)"
FT /evidence="ECO:0000269|Ref.2, ECO:0000269|Ref.3"
SQ SEQUENCE 348 AA; 38134 MW; D00B5E43431875D7 CRC64;
MDNSSVCPPN ATVCEGDSCV VPESNFNAIL NTVMSTVLTI LLAMVMFSMG CNVEVHKFLG
HIKRPWGIFV GFLCQFGIMP LTGFILSVAS GILPVQAVVV LIMGCCPGGT GSNILAYWID
GDMDLSVSMT TCSTLLALGM MPLCLFVYTK MWVDSGTIVI PYDSIGISLV ALVIPVSFGM
FVNHKWPQKA KIILKIGSIT GVILIVLIAV IGGILYQSAW IIEPKLWIIG TIFPIAGYSL
GFFLARLAGQ PWYRCRTVAL ETGMQNTQLC STIVQLSFSP EDLNLVFTFP LIYTVFQLVF
AAVILGIYVT YRKCYGKNDA EFLEKTDNEM DSRPSFDETN KGFQPDEK
