NTCP4_HUMAN
ID NTCP4_HUMAN Reviewed; 437 AA.
AC Q96EP9; Q8WUZ2;
DT 12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2004, sequence version 2.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Sodium/bile acid cotransporter 4;
DE AltName: Full=Na(+)/bile acid cotransporter 4;
DE AltName: Full=Solute carrier family 10 member 4;
GN Name=SLC10A4;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Mikkaichi T., Natori M., Abe T., Hishinuma T., Goto J.;
RT "Identification of a novel bile acid transporter in human, SLC10A4.";
RL Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP TISSUE SPECIFICITY.
RX PubMed=18355966; DOI=10.1016/j.neuroscience.2008.01.049;
RA Geyer J., Fernandes C.F., Doring B., Burger S., Godoy J.R., Rafalzik S.,
RA Hubschle T., Gerstberger R., Petzinger E.;
RT "Cloning and molecular characterization of the orphan carrier protein
RT Slc10a4: expression in cholinergic neurons of the rat central nervous
RT system.";
RL Neuroscience 152:990-1005(2008).
RN [4]
RP FUNCTION, PROTEOLYTIC CLEAVAGE, AND SUBCELLULAR LOCATION.
RX PubMed=23589386; DOI=10.1093/jb/mvt031;
RA Abe T., Kanemitu Y., Nakasone M., Kawahata I., Yamakuni T., Nakajima A.,
RA Suzuki N., Nishikawa M., Hishinuma T., Tomioka Y.;
RT "SLC10A4 is a protease-activated transporter that transports bile acids.";
RL J. Biochem. 154:93-101(2013).
CC -!- FUNCTION: Transporter for bile acids. {ECO:0000269|PubMed:23589386}.
CC -!- INTERACTION:
CC Q96EP9; P29400-2: COL4A5; NbExp=3; IntAct=EBI-17456472, EBI-12211159;
CC Q96EP9; Q96LL9: DNAJC30; NbExp=3; IntAct=EBI-17456472, EBI-8639143;
CC Q96EP9; Q96G79: SLC35A4; NbExp=3; IntAct=EBI-17456472, EBI-12363689;
CC Q96EP9; Q8N511: TMEM199; NbExp=3; IntAct=EBI-17456472, EBI-10265825;
CC Q96EP9; A2RU14: TMEM218; NbExp=3; IntAct=EBI-17456472, EBI-10173151;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:23589386};
CC Multi-pass membrane protein {ECO:0000269|PubMed:23589386}.
CC -!- TISSUE SPECIFICITY: Highly expressed in brain and small intestine, and
CC moderately expressed in colon, heart, prostate, and testis. Very low
CC levels were detected in kidney, liver, ovary, placenta, spleen, and
CC thymus. {ECO:0000269|PubMed:18355966}.
CC -!- PTM: Activated following N-terminal proteolytic cleavage by thrombin
CC and/or proteases. {ECO:0000269|PubMed:23589386}.
CC -!- SIMILARITY: Belongs to the bile acid:sodium symporter (BASS) (TC
CC 2.A.28) family. {ECO:0000305}.
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DR EMBL; AY704414; AAW30130.1; -; mRNA.
DR EMBL; BC012048; AAH12048.2; -; mRNA.
DR EMBL; BC019066; AAH19066.1; -; mRNA.
DR CCDS; CCDS3482.1; -.
DR RefSeq; NP_689892.1; NM_152679.3.
DR AlphaFoldDB; Q96EP9; -.
DR SMR; Q96EP9; -.
DR BioGRID; 128402; 5.
DR IntAct; Q96EP9; 6.
DR STRING; 9606.ENSP00000273861; -.
DR TCDB; 2.A.28.1.7; the bile acid:na(+) symporter (bass) family.
DR GlyGen; Q96EP9; 5 sites.
DR iPTMnet; Q96EP9; -.
DR PhosphoSitePlus; Q96EP9; -.
DR BioMuta; SLC10A4; -.
DR DMDM; 74731592; -.
DR jPOST; Q96EP9; -.
DR MassIVE; Q96EP9; -.
DR MaxQB; Q96EP9; -.
DR PaxDb; Q96EP9; -.
DR PeptideAtlas; Q96EP9; -.
DR PRIDE; Q96EP9; -.
DR ProteomicsDB; 76439; -.
DR Antibodypedia; 23816; 97 antibodies from 21 providers.
DR DNASU; 201780; -.
DR Ensembl; ENST00000273861.5; ENSP00000273861.4; ENSG00000145248.7.
DR GeneID; 201780; -.
DR KEGG; hsa:201780; -.
DR MANE-Select; ENST00000273861.5; ENSP00000273861.4; NM_152679.4; NP_689892.1.
DR UCSC; uc003gyc.3; human.
DR CTD; 201780; -.
DR GeneCards; SLC10A4; -.
DR HGNC; HGNC:22980; SLC10A4.
DR HPA; ENSG00000145248; Tissue enriched (brain).
DR MIM; 618563; gene.
DR neXtProt; NX_Q96EP9; -.
DR OpenTargets; ENSG00000145248; -.
DR PharmGKB; PA134941471; -.
DR VEuPathDB; HostDB:ENSG00000145248; -.
DR eggNOG; KOG2718; Eukaryota.
DR GeneTree; ENSGT00950000182808; -.
DR HOGENOM; CLU_034788_7_3_1; -.
DR InParanoid; Q96EP9; -.
DR OMA; NIMMETT; -.
DR OrthoDB; 1148347at2759; -.
DR PhylomeDB; Q96EP9; -.
DR TreeFam; TF315811; -.
DR PathwayCommons; Q96EP9; -.
DR SignaLink; Q96EP9; -.
DR BioGRID-ORCS; 201780; 7 hits in 1074 CRISPR screens.
DR GenomeRNAi; 201780; -.
DR Pharos; Q96EP9; Tbio.
DR PRO; PR:Q96EP9; -.
DR Proteomes; UP000005640; Chromosome 4.
DR RNAct; Q96EP9; protein.
DR Bgee; ENSG00000145248; Expressed in substantia nigra pars reticulata and 111 other tissues.
DR Genevisible; Q96EP9; HS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008508; F:bile acid:sodium symporter activity; IBA:GO_Central.
DR GO; GO:0015721; P:bile acid and bile salt transport; IBA:GO_Central.
DR Gene3D; 1.20.1530.20; -; 1.
DR InterPro; IPR002657; BilAc:Na_symport/Acr3.
DR InterPro; IPR004710; Bilac:Na_transpt.
DR InterPro; IPR038770; Na+/solute_symporter_sf.
DR InterPro; IPR030204; SLC10A4.
DR PANTHER; PTHR10361; PTHR10361; 1.
DR PANTHER; PTHR10361:SF41; PTHR10361:SF41; 1.
DR Pfam; PF01758; SBF; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Glycoprotein; Ion transport; Membrane; Reference proteome;
KW Sodium; Sodium transport; Symport; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..437
FT /note="Sodium/bile acid cotransporter 4"
FT /id="PRO_0000263742"
FT TOPO_DOM 1..103
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..124
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 125..140
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 141..161
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 162..197
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 198..218
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 219..233
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 234..254
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 255..267
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 268..288
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 289..291
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 292..312
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 313..360
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 361..381
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 382..437
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 37..82
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 87..88
FT /note="Cleavage; by thrombin"
FT CARBOHYD 6
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 18
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 24
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 181
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 195
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 437 AA; 46504 MW; 055E989629CC13D1 CRC64;
MDGNDNVTLL FAPLLRDNYT LAPNASSLGP GTDLALAPAS SAGPGPGLSL GPGPSFGFSP
GPTPTPEPTT SGLAGGAASH GPSPFPRPWA PHALPFWDTP LNHGLNVFVG AALCITMLGL
GCTVDVNHFG AHVRRPVGAL LAALCQFGLL PLLAFLLALA FKLDEVAAVA VLLCGCCPGG
NLSNLMSLLV DGDMNLSIIM TISSTLLALV LMPLCLWIYS WAWINTPIVQ LLPLGTVTLT
LCSTLIPIGL GVFIRYKYSR VADYIVKVSL WSLLVTLVVL FIMTGTMLGP ELLASIPAAV
YVIAIFMPLA GYASGYGLAT LFHLPPNCKR TVCLETGSQN VQLCTAILKL AFPPQFIGSM
YMFPLLYALF QSAEAGIFVL IYKMYGSEML HKRDPLDEDE DTDISYKKLK EEEMADTSYG
TVKAENIIMM ETAQTSL
