NTCP4_MOUSE
ID NTCP4_MOUSE Reviewed; 437 AA.
AC Q3UEZ8; Q8BJC7;
DT 12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Sodium/bile acid cotransporter 4;
DE AltName: Full=Na(+)/bile acid cotransporter 4;
DE AltName: Full=Solute carrier family 10 member 4;
GN Name=Slc10a4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Eye, and Sympathetic ganglion;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP TISSUE SPECIFICITY.
RX PubMed=18355966; DOI=10.1016/j.neuroscience.2008.01.049;
RA Geyer J., Fernandes C.F., Doring B., Burger S., Godoy J.R., Rafalzik S.,
RA Hubschle T., Gerstberger R., Petzinger E.;
RT "Cloning and molecular characterization of the orphan carrier protein
RT Slc10a4: expression in cholinergic neurons of the rat central nervous
RT system.";
RL Neuroscience 152:990-1005(2008).
CC -!- FUNCTION: Transporter for bile acids. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Highest expression in the brain and significantly
CC above background levels in the eye, prostate, and whole embryo tissue
CC preparations. {ECO:0000269|PubMed:18355966}.
CC -!- PTM: Activated following N-terminal proteolytic cleavage by thrombin
CC and/or proteases. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the bile acid:sodium symporter (BASS) (TC
CC 2.A.28) family. {ECO:0000305}.
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DR EMBL; AK087479; BAC39890.1; -; mRNA.
DR EMBL; AK149202; BAE28763.1; -; mRNA.
DR CCDS; CCDS19336.1; -.
DR RefSeq; NP_775579.2; NM_173403.2.
DR AlphaFoldDB; Q3UEZ8; -.
DR SMR; Q3UEZ8; -.
DR STRING; 10090.ENSMUSP00000031127; -.
DR GlyGen; Q3UEZ8; 4 sites.
DR PhosphoSitePlus; Q3UEZ8; -.
DR PaxDb; Q3UEZ8; -.
DR PRIDE; Q3UEZ8; -.
DR ProteomicsDB; 253031; -.
DR DNASU; 231290; -.
DR Ensembl; ENSMUST00000031127; ENSMUSP00000031127; ENSMUSG00000029219.
DR GeneID; 231290; -.
DR KEGG; mmu:231290; -.
DR UCSC; uc008xsk.1; mouse.
DR CTD; 201780; -.
DR MGI; MGI:3606480; Slc10a4.
DR VEuPathDB; HostDB:ENSMUSG00000029219; -.
DR eggNOG; KOG2718; Eukaryota.
DR GeneTree; ENSGT00950000182808; -.
DR HOGENOM; CLU_034788_7_3_1; -.
DR InParanoid; Q3UEZ8; -.
DR OMA; NIMMETT; -.
DR OrthoDB; 1148347at2759; -.
DR PhylomeDB; Q3UEZ8; -.
DR TreeFam; TF315811; -.
DR BioGRID-ORCS; 231290; 1 hit in 74 CRISPR screens.
DR PRO; PR:Q3UEZ8; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q3UEZ8; protein.
DR Bgee; ENSMUSG00000029219; Expressed in neural tube and 61 other tissues.
DR ExpressionAtlas; Q3UEZ8; baseline and differential.
DR Genevisible; Q3UEZ8; MM.
DR GO; GO:0098981; C:cholinergic synapse; IDA:SynGO.
DR GO; GO:0098691; C:dopaminergic synapse; IDA:SynGO.
DR GO; GO:0030285; C:integral component of synaptic vesicle membrane; IDA:SynGO.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0099154; C:serotonergic synapse; IDA:SynGO.
DR GO; GO:0008508; F:bile acid:sodium symporter activity; IBA:GO_Central.
DR GO; GO:0030534; P:adult behavior; IMP:MGI.
DR GO; GO:0015721; P:bile acid and bile salt transport; IBA:GO_Central.
DR GO; GO:0099162; P:regulation of neurotransmitter loading into synaptic vesicle; IDA:SynGO.
DR GO; GO:0009410; P:response to xenobiotic stimulus; IMP:MGI.
DR Gene3D; 1.20.1530.20; -; 1.
DR InterPro; IPR002657; BilAc:Na_symport/Acr3.
DR InterPro; IPR004710; Bilac:Na_transpt.
DR InterPro; IPR038770; Na+/solute_symporter_sf.
DR InterPro; IPR030204; SLC10A4.
DR PANTHER; PTHR10361; PTHR10361; 1.
DR PANTHER; PTHR10361:SF41; PTHR10361:SF41; 1.
DR Pfam; PF01758; SBF; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Glycoprotein; Ion transport; Membrane; Reference proteome;
KW Sodium; Sodium transport; Symport; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..437
FT /note="Sodium/bile acid cotransporter 4"
FT /id="PRO_0000263743"
FT TOPO_DOM 1..103
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..124
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 125..140
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 141..161
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 162..197
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 198..218
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 219..233
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 234..254
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 255..267
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 268..288
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 289..291
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 292..312
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 313..360
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 361..381
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 382..437
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 15..79
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 15..49
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 56..79
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 87..88
FT /note="Cleavage; by thrombin"
FT /evidence="ECO:0000250"
FT CARBOHYD 6
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 20
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 181
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 195
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 422
FT /note="V -> G (in Ref. 1; BAC39890)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 437 AA; 46642 MW; F3F4A0A79C4CE516 CRC64;
MDSLDNTTLL LAPSSLLPDN LTLSPNAGSP SASTLSPLAV TSSPGPGLSL APSPSIGFSP
EATPTPEPTS SSLTVGVAGQ GSSAFPRPWI PHEPPFWDTP LNHGLNVFVG AALCITMLGL
GCTVDVNHFG AHVRRPVGAL LAALCQFGFL PLLAFLLALI FKLDEVAAVA VLLCGCCPGG
NLSNLMSLLV DGDMNLSIIM TISSTLLALV LMPLCLWIYS RAWINTPLVQ LLPLGAVTLT
LCSTLIPIGL GVFIRYKYNR VADYIVKVSL WSLLVTLVVL FIMTGTMLGP ELLASIPATV
YVVAIFMPLA GYASGYGLAT LFHLPPNCKR TVCLETGSQN VQLCTAILKL AFPPRFIGSM
YMFPLLYALF QSAEAGVFVL IYKMYGSEIL HKREALDEDE DTDISYKKLK EEEMADTSYG
TVGTDDLVMM ETTQTAL
