NTCP_MOUSE
ID NTCP_MOUSE Reviewed; 362 AA.
AC O08705; Q52L86;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=Sodium/bile acid cotransporter;
DE AltName: Full=Na(+)/bile acid cotransporter;
DE AltName: Full=Na(+)/taurocholate transport protein;
DE AltName: Full=Sodium/taurocholate cotransporting polypeptide;
DE AltName: Full=Solute carrier family 10 member 1;
GN Name=Slc10a1; Synonyms=Ntcp;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=BALB/cJ; TISSUE=Liver;
RA Saeki T.;
RL Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=BALB/cJ;
RA Hagenbuch B.;
RL Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Liver;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-330, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-330, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: As a major transporter of conjugated bile salts from plasma
CC into the hepatocyte, it has a key role in the enterohepatic circulation
CC of bile salts necessary for the solubilization and absorption of
CC dietary fat and fat-soluble vitamins. It exhibits broad substrate
CC specificity and transports various non-bile acid organic compounds as
CC well. It is strictly dependent on the extracellular presence of sodium.
CC Able to transport taurocholate, cholate, and the non-bile acid estron
CC sulfate. {ECO:0000250|UniProtKB:Q14973}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q14973};
CC Multi-pass membrane protein {ECO:0000250|UniProtKB:Q14973}.
CC -!- SIMILARITY: Belongs to the bile acid:sodium symporter (BASS) (TC
CC 2.A.28) family. {ECO:0000305}.
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DR EMBL; AB003303; BAA19846.1; -; mRNA.
DR EMBL; U95131; AAB81023.1; -; mRNA.
DR EMBL; BC021154; AAH21154.1; -; mRNA.
DR EMBL; BC094023; AAH94023.1; -; mRNA.
DR CCDS; CCDS49101.1; -.
DR RefSeq; NP_001171032.1; NM_001177561.1.
DR RefSeq; XP_006515696.1; XM_006515633.3.
DR AlphaFoldDB; O08705; -.
DR SMR; O08705; -.
DR STRING; 10090.ENSMUSP00000093229; -.
DR ChEMBL; CHEMBL2073713; -.
DR GlyGen; O08705; 5 sites.
DR iPTMnet; O08705; -.
DR PhosphoSitePlus; O08705; -.
DR jPOST; O08705; -.
DR MaxQB; O08705; -.
DR PaxDb; O08705; -.
DR PeptideAtlas; O08705; -.
DR PRIDE; O08705; -.
DR ProteomicsDB; 287832; -.
DR Antibodypedia; 47330; 225 antibodies from 28 providers.
DR DNASU; 20493; -.
DR Ensembl; ENSMUST00000095572; ENSMUSP00000093229; ENSMUSG00000021135.
DR Ensembl; ENSMUST00000218162; ENSMUSP00000151555; ENSMUSG00000021135.
DR GeneID; 20493; -.
DR KEGG; mmu:20493; -.
DR UCSC; uc011yoj.1; mouse.
DR CTD; 6554; -.
DR MGI; MGI:97379; Slc10a1.
DR VEuPathDB; HostDB:ENSMUSG00000021135; -.
DR eggNOG; KOG2718; Eukaryota.
DR GeneTree; ENSGT00950000182808; -.
DR HOGENOM; CLU_034788_7_5_1; -.
DR InParanoid; O08705; -.
DR OMA; MEIGIHN; -.
DR OrthoDB; 1148347at2759; -.
DR PhylomeDB; O08705; -.
DR TreeFam; TF315811; -.
DR Reactome; R-MMU-159418; Recycling of bile acids and salts.
DR BioGRID-ORCS; 20493; 4 hits in 75 CRISPR screens.
DR ChiTaRS; Slc10a1; mouse.
DR PRO; PR:O08705; -.
DR Proteomes; UP000000589; Chromosome 12.
DR RNAct; O08705; protein.
DR Bgee; ENSMUSG00000021135; Expressed in left lobe of liver and 50 other tissues.
DR ExpressionAtlas; O08705; baseline and differential.
DR Genevisible; O08705; MM.
DR GO; GO:0016323; C:basolateral plasma membrane; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IDA:MGI.
DR GO; GO:0015125; F:bile acid transmembrane transporter activity; ISO:MGI.
DR GO; GO:0008508; F:bile acid:sodium symporter activity; ISO:MGI.
DR GO; GO:0015721; P:bile acid and bile salt transport; ISO:MGI.
DR GO; GO:0038183; P:bile acid signaling pathway; IEA:Ensembl.
DR GO; GO:0071466; P:cellular response to xenobiotic stimulus; IEA:Ensembl.
DR GO; GO:0120188; P:regulation of bile acid secretion; IEA:Ensembl.
DR GO; GO:0010468; P:regulation of gene expression; IEA:Ensembl.
DR GO; GO:1904486; P:response to 17alpha-ethynylestradiol; IEA:Ensembl.
DR GO; GO:0045471; P:response to ethanol; IEA:Ensembl.
DR GO; GO:0031667; P:response to nutrient levels; IEA:Ensembl.
DR Gene3D; 1.20.1530.20; -; 1.
DR InterPro; IPR002657; BilAc:Na_symport/Acr3.
DR InterPro; IPR004710; Bilac:Na_transpt.
DR InterPro; IPR038770; Na+/solute_symporter_sf.
DR InterPro; IPR030208; SLC10A1.
DR PANTHER; PTHR10361; PTHR10361; 1.
DR PANTHER; PTHR10361:SF40; PTHR10361:SF40; 1.
DR Pfam; PF01758; SBF; 1.
DR TIGRFAMs; TIGR00841; bass; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Glycoprotein; Ion transport; Membrane; Phosphoprotein;
KW Reference proteome; Sodium; Sodium transport; Symport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..362
FT /note="Sodium/bile acid cotransporter"
FT /id="PRO_0000052336"
FT TRANSMEM 24..45
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 60..80
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 82..98
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 158..178
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 190..211
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 228..244
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 285..306
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 332..362
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 336..362
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 330
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT CARBOHYD 5
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 11
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 103
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 117
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 271
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 362 AA; 39413 MW; 7A70493E1804280F CRC64;
MEAHNVSAPF NFSLPPGFGH RATDTALSVI LVVMLLLIML SLGCTMEFSK IKAHFWKPKG
VIIAIVAQYG IMPLSAFLLG KVFHLTSIEA LAILICGCSP GGNLSNLFTL AMKGDMNLSI
VMTTCSSFTA LGMMPLLLYI YSKGIYDGDL KDKVPYKGIM LSLVMVLIPC AIGIFLKSKR
PHYVPYVLKA GMIITFSLSV AVTVLSVINV GNSIMFVMTP HLLATSSLMP FTGFLMGYIL
SALFRLNPSC RRTISMETGF QNVQLCSTIL NVTFPPEVIG PLFFFPLLYM IFQLAEGLLF
IIIFRCYLKI KPQKDQTKIT YKAAATEDAT PAALEKGTHN GNNPPTQPGL SPNGLNSGQM
AN
