NTCP_RAT
ID NTCP_RAT Reviewed; 362 AA.
AC P26435; Q5BK99;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1992, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Sodium/bile acid cotransporter;
DE AltName: Full=Na(+)/bile acid cotransporter;
DE AltName: Full=Na(+)/taurocholate transport protein;
DE AltName: Full=Sodium/taurocholate cotransporting polypeptide;
DE AltName: Full=Solute carrier family 10 member 1;
GN Name=Slc10a1; Synonyms=Ntcp;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Liver;
RX PubMed=1961729; DOI=10.1073/pnas.88.23.10629;
RA Hagenbuch B., Stieger B., Foguet M., Luebbert H., Meier P.J.;
RT "Functional expression cloning and characterization of the hepatocyte
RT Na+/bile acid cotransport system.";
RL Proc. Natl. Acad. Sci. U.S.A. 88:10629-10633(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Liver;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-330, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: As a major transporter of conjugated bile salts from plasma
CC into the hepatocyte, it has a key role in the enterohepatic circulation
CC of bile salts necessary for the solubilization and absorption of
CC dietary fat and fat-soluble vitamins. It exhibits broad substrate
CC specificity and transports various non-bile acid organic compounds as
CC well. It is strictly dependent on the extracellular presence of sodium.
CC Able to transport taurocholate, cholate, and the non-bile acid estron
CC sulfate. {ECO:0000250|UniProtKB:Q14973}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q14973};
CC Multi-pass membrane protein {ECO:0000250|UniProtKB:Q14973}.
CC -!- TISSUE SPECIFICITY: Liver and kidney.
CC -!- SIMILARITY: Belongs to the bile acid:sodium symporter (BASS) (TC
CC 2.A.28) family. {ECO:0000305}.
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DR EMBL; M77479; AAA42112.1; -; mRNA.
DR EMBL; BC091152; AAH91152.1; -; mRNA.
DR PIR; A41601; A41601.
DR RefSeq; NP_058743.1; NM_017047.1.
DR AlphaFoldDB; P26435; -.
DR SMR; P26435; -.
DR IntAct; P26435; 2.
DR STRING; 10116.ENSRNOP00000007825; -.
DR BindingDB; P26435; -.
DR ChEMBL; CHEMBL4847; -.
DR TCDB; 2.A.28.1.1; the bile acid:na(+) symporter (bass) family.
DR GlyGen; P26435; 5 sites.
DR iPTMnet; P26435; -.
DR PhosphoSitePlus; P26435; -.
DR PaxDb; P26435; -.
DR PRIDE; P26435; -.
DR Ensembl; ENSRNOT00000007825; ENSRNOP00000007825; ENSRNOG00000005794.
DR GeneID; 24777; -.
DR KEGG; rno:24777; -.
DR UCSC; RGD:3681; rat.
DR CTD; 6554; -.
DR RGD; 3681; Slc10a1.
DR eggNOG; KOG2718; Eukaryota.
DR GeneTree; ENSGT00950000182808; -.
DR HOGENOM; CLU_034788_7_5_1; -.
DR InParanoid; P26435; -.
DR OMA; MEIGIHN; -.
DR OrthoDB; 1148347at2759; -.
DR PhylomeDB; P26435; -.
DR TreeFam; TF315811; -.
DR Reactome; R-RNO-159418; Recycling of bile acids and salts.
DR SABIO-RK; P26435; -.
DR PRO; PR:P26435; -.
DR Proteomes; UP000002494; Chromosome 6.
DR Bgee; ENSRNOG00000005794; Expressed in liver and 16 other tissues.
DR Genevisible; P26435; RN.
DR GO; GO:0016323; C:basolateral plasma membrane; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; ISO:RGD.
DR GO; GO:0015125; F:bile acid transmembrane transporter activity; IDA:RGD.
DR GO; GO:0008508; F:bile acid:sodium symporter activity; IDA:RGD.
DR GO; GO:0015721; P:bile acid and bile salt transport; IDA:RGD.
DR GO; GO:0038183; P:bile acid signaling pathway; IEP:RGD.
DR GO; GO:0071466; P:cellular response to xenobiotic stimulus; IEP:RGD.
DR GO; GO:0120188; P:regulation of bile acid secretion; IEP:RGD.
DR GO; GO:0010468; P:regulation of gene expression; IEP:RGD.
DR GO; GO:1904486; P:response to 17alpha-ethynylestradiol; IEP:RGD.
DR GO; GO:0045471; P:response to ethanol; IEP:RGD.
DR GO; GO:0031667; P:response to nutrient levels; IEP:RGD.
DR GO; GO:0014070; P:response to organic cyclic compound; IEP:RGD.
DR Gene3D; 1.20.1530.20; -; 1.
DR InterPro; IPR002657; BilAc:Na_symport/Acr3.
DR InterPro; IPR004710; Bilac:Na_transpt.
DR InterPro; IPR038770; Na+/solute_symporter_sf.
DR InterPro; IPR030208; SLC10A1.
DR PANTHER; PTHR10361; PTHR10361; 1.
DR PANTHER; PTHR10361:SF40; PTHR10361:SF40; 1.
DR Pfam; PF01758; SBF; 1.
DR TIGRFAMs; TIGR00841; bass; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Glycoprotein; Ion transport; Membrane; Phosphoprotein;
KW Reference proteome; Sodium; Sodium transport; Symport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..362
FT /note="Sodium/bile acid cotransporter"
FT /id="PRO_0000052337"
FT TRANSMEM 24..45
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 60..80
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 82..98
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 158..178
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 190..211
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 228..244
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 285..306
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 333..362
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 330
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT CARBOHYD 5
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 11
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 103
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 117
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 271
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 362 AA; 39295 MW; F0ABB76076A57550 CRC64;
MEVHNVSAPF NFSLPPGFGH RATDKALSII LVLMLLLIML SLGCTMEFSK IKAHLWKPKG
VIVALVAQFG IMPLAAFLLG KIFHLSNIEA LAILICGCSP GGNLSNLFTL AMKGDMNLSI
VMTTCSSFSA LGMMPLLLYV YSKGIYDGDL KDKVPYKGIM ISLVIVLIPC TIGIVLKSKR
PHYVPYILKG GMIITFLLSV AVTALSVINV GNSIMFVMTP HLLATSSLMP FSGFLMGYIL
SALFQLNPSC RRTISMETGF QNIQLCSTIL NVTFPPEVIG PLFFFPLLYM IFQLAEGLLI
IIIFRCYEKI KPPKDQTKIT YKAAATEDAT PAALEKGTHN GNIPPLQPGP SPNGLNSGQM
AN
