NTF2B_ARATH
ID NTF2B_ARATH Reviewed; 126 AA.
AC Q9C7F5; Q1H566;
DT 14-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 25-MAY-2022, entry version 114.
DE RecName: Full=Nuclear transport factor 2B {ECO:0000303|PubMed:16428596};
DE Short=AtNTF2b {ECO:0000303|PubMed:16428596};
DE Short=NTF-2;
GN Name=NTF2B {ECO:0000303|PubMed:16428596};
GN OrderedLocusNames=At1g27970 {ECO:0000312|Araport:AT1G27970};
GN ORFNames=F13K9.26 {ECO:0000312|EMBL:AAG51491.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH RAN1, SUBCELLULAR LOCATION,
RP GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=16428596; DOI=10.1104/pp.105.075499;
RA Zhao Q., Leung S., Corbett A.H., Meier I.;
RT "Identification and characterization of the Arabidopsis orthologs of
RT nuclear transport factor 2, the nuclear import factor of ran.";
RL Plant Physiol. 140:869-878(2006).
RN [5]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
CC -!- FUNCTION: Facilitates protein transport into the nucleus. Interacts
CC with various nucleoporins and with Ran-GDP. Could be part of a
CC multicomponent system of cytosolic factors that assemble at the pore
CC complex during nuclear import. {ECO:0000269|PubMed:16428596}.
CC -!- SUBUNIT: Interacts with RAN1. {ECO:0000269|PubMed:16428596}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16428596}. Nucleus
CC {ECO:0000269|PubMed:16428596}. Nucleus envelope
CC {ECO:0000269|PubMed:16428596}. Note=Accumulates at the nuclear rim.
CC Excluded from the nucleolus. {ECO:0000269|PubMed:16428596}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9C7F5-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Expressed in roots, stems, leaves and flowers, and,
CC at low levels, in siliques. {ECO:0000269|PubMed:16428596}.
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DR EMBL; AC069471; AAG51491.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30896.1; -; Genomic_DNA.
DR EMBL; BT025598; ABF59016.1; -; mRNA.
DR PIR; B86405; B86405.
DR RefSeq; NP_174118.1; NM_102562.4. [Q9C7F5-1]
DR AlphaFoldDB; Q9C7F5; -.
DR SMR; Q9C7F5; -.
DR BioGRID; 24925; 5.
DR STRING; 3702.AT1G27970.2; -.
DR iPTMnet; Q9C7F5; -.
DR PaxDb; Q9C7F5; -.
DR PRIDE; Q9C7F5; -.
DR ProteomicsDB; 248938; -. [Q9C7F5-1]
DR EnsemblPlants; AT1G27970.1; AT1G27970.1; AT1G27970. [Q9C7F5-1]
DR GeneID; 839690; -.
DR Gramene; AT1G27970.1; AT1G27970.1; AT1G27970. [Q9C7F5-1]
DR KEGG; ath:AT1G27970; -.
DR Araport; AT1G27970; -.
DR eggNOG; KOG2104; Eukaryota.
DR HOGENOM; CLU_131642_0_0_1; -.
DR InParanoid; Q9C7F5; -.
DR OMA; FTEFYYN; -.
DR PhylomeDB; Q9C7F5; -.
DR PRO; PR:Q9C7F5; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C7F5; baseline and differential.
DR Genevisible; Q9C7F5; AT.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005635; C:nuclear envelope; IDA:UniProtKB.
DR GO; GO:0044613; C:nuclear pore central transport channel; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0006913; P:nucleocytoplasmic transport; IBA:GO_Central.
DR GO; GO:0006606; P:protein import into nucleus; IDA:UniProtKB.
DR CDD; cd00780; NTF2; 1.
DR InterPro; IPR032710; NTF2-like_dom_sf.
DR InterPro; IPR045875; NTF2/Mtr2.
DR InterPro; IPR002075; NTF2_dom.
DR InterPro; IPR018222; Nuclear_transport_factor_2_euk.
DR PANTHER; PTHR12612; PTHR12612; 1.
DR Pfam; PF02136; NTF2; 1.
DR SUPFAM; SSF54427; SSF54427; 1.
DR PROSITE; PS50177; NTF2_DOMAIN; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Cytoplasm; Nucleus; Protein transport;
KW Reference proteome; Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22223895"
FT CHAIN 2..126
FT /note="Nuclear transport factor 2B"
FT /id="PRO_0000194780"
FT DOMAIN 9..123
FT /note="NTF2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00137"
FT MOD_RES 2
FT /note="N-acetylserine"
FT /evidence="ECO:0007744|PubMed:22223895"
SQ SEQUENCE 126 AA; 14002 MW; 263E06A7A8903E3B CRC64;
MSQMDPDAVS KAFVEHYYST FDTNRVGLAG LYQEASMLTF EGQKIQGVQS IVAKLTSLPF
QQCKHHISTV DCQPSGPASG MLVFVSGNLQ LAGEEHALKF SQMFHLMPTP QGSFYVFNDI
FRLNYA
