NTF2_MOUSE
ID NTF2_MOUSE Reviewed; 127 AA.
AC P61971; P13662; Q3TI35;
DT 07-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT 07-JUN-2004, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Nuclear transport factor 2 {ECO:0000305};
DE Short=NTF-2;
GN Name=Nutf2 {ECO:0000312|MGI:MGI:1915301};
GN Synonyms=Ntf2 {ECO:0000250|UniProtKB:P61972};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and DBA/2J; TISSUE=Kidney;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Mediates the import of GDP-bound RAN from the cytoplasm into
CC the nucleus which is essential for the function of RAN in cargo
CC receptor-mediated nucleocytoplasmic transport. Thereby, plays
CC indirectly a more general role in cargo receptor-mediated
CC nucleocytoplasmic transport. Interacts with GDP-bound RAN in the
CC cytosol, recruits it to the nuclear pore complex via its interaction
CC with nucleoporins and promotes its nuclear import.
CC {ECO:0000250|UniProtKB:P61970}.
CC -!- SUBUNIT: Homodimer. Interacts with RAN (GDP-bound form); the
CC interaction is direct and regulates RAN nuclear import. Interacts with
CC the nucleoporins NUP54, NUP58 and NUP62 (via FG repeats); recruits
CC NUTF2 to the nuclear pore complex a step required for NUTF2-mediated
CC GDP-bound RAN nuclear import. Interacts with CAPG; mediates its nuclear
CC import. {ECO:0000250|UniProtKB:P61970}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC {ECO:0000250|UniProtKB:P61970}. Nucleus outer membrane
CC {ECO:0000250|UniProtKB:P61972}. Nucleus, nuclear pore complex
CC {ECO:0000250|UniProtKB:P61972}. Nucleus inner membrane
CC {ECO:0000250|UniProtKB:P61972}. Nucleus, nucleoplasm
CC {ECO:0000250|UniProtKB:P61970}. Note=At steady state it is essentially
CC nucleoplasmic, enriched in nucleoplasmic foci.
CC {ECO:0000250|UniProtKB:P61970}.
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DR EMBL; AK002461; BAB22117.1; -; mRNA.
DR EMBL; AK012500; BAB28283.1; -; mRNA.
DR EMBL; AK020512; BAB32122.1; -; mRNA.
DR EMBL; AK028410; BAC25936.1; -; mRNA.
DR EMBL; AK051049; BAC34511.1; -; mRNA.
DR EMBL; AK168026; BAE40011.1; -; mRNA.
DR EMBL; BC003955; AAH03955.1; -; mRNA.
DR EMBL; BC083165; AAH83165.1; -; mRNA.
DR EMBL; BC086773; AAH86773.1; -; mRNA.
DR CCDS; CCDS22617.1; -.
DR RefSeq; NP_080808.1; NM_026532.3.
DR RefSeq; XP_001474007.1; XM_001473957.5.
DR RefSeq; XP_006531388.1; XM_006531325.3.
DR AlphaFoldDB; P61971; -.
DR BMRB; P61971; -.
DR SMR; P61971; -.
DR BioGRID; 212629; 4.
DR STRING; 10090.ENSMUSP00000008594; -.
DR iPTMnet; P61971; -.
DR PhosphoSitePlus; P61971; -.
DR SwissPalm; P61971; -.
DR EPD; P61971; -.
DR jPOST; P61971; -.
DR MaxQB; P61971; -.
DR PaxDb; P61971; -.
DR PeptideAtlas; P61971; -.
DR PRIDE; P61971; -.
DR ProteomicsDB; 287833; -.
DR DNASU; 68051; -.
DR Ensembl; ENSMUST00000008594; ENSMUSP00000008594; ENSMUSG00000008450.
DR Ensembl; ENSMUST00000095978; ENSMUSP00000131850; ENSMUSG00000071497.
DR Ensembl; ENSMUST00000212042; ENSMUSP00000148713; ENSMUSG00000008450.
DR GeneID; 68051; -.
DR KEGG; mmu:68051; -.
DR UCSC; uc009nej.1; mouse.
DR CTD; 10204; -.
DR MGI; MGI:1915301; Nutf2.
DR VEuPathDB; HostDB:ENSMUSG00000008450; -.
DR VEuPathDB; HostDB:ENSMUSG00000071497; -.
DR eggNOG; KOG2104; Eukaryota.
DR GeneTree; ENSGT00510000047030; -.
DR HOGENOM; CLU_131642_1_1_1; -.
DR InParanoid; P61971; -.
DR OMA; FTEFYYN; -.
DR OrthoDB; 1437819at2759; -.
DR PhylomeDB; P61971; -.
DR TreeFam; TF314422; -.
DR BioGRID-ORCS; 68051; 25 hits in 53 CRISPR screens.
DR ChiTaRS; Nutf2; mouse.
DR PRO; PR:P61971; -.
DR Proteomes; UP000000589; Chromosome 19.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; P61971; protein.
DR Bgee; ENSMUSG00000008450; Expressed in yolk sac and 279 other tissues.
DR ExpressionAtlas; P61971; baseline and differential.
DR Genevisible; P61971; MM.
DR GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR GO; GO:0005637; C:nuclear inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0031965; C:nuclear membrane; ISS:UniProtKB.
DR GO; GO:0005640; C:nuclear outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0044613; C:nuclear pore central transport channel; IBA:GO_Central.
DR GO; GO:0005654; C:nucleoplasm; ISS:UniProtKB.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR GO; GO:0061608; F:nuclear import signal receptor activity; ISS:UniProtKB.
DR GO; GO:0031267; F:small GTPase binding; ISS:UniProtKB.
DR GO; GO:0017056; F:structural constituent of nuclear pore; ISO:MGI.
DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR GO; GO:0006913; P:nucleocytoplasmic transport; IBA:GO_Central.
DR GO; GO:0006611; P:protein export from nucleus; ISO:MGI.
DR GO; GO:0006606; P:protein import into nucleus; IDA:MGI.
DR GO; GO:0090204; P:protein localization to nuclear pore; ISS:UniProtKB.
DR CDD; cd00780; NTF2; 1.
DR InterPro; IPR032710; NTF2-like_dom_sf.
DR InterPro; IPR045875; NTF2/Mtr2.
DR InterPro; IPR002075; NTF2_dom.
DR InterPro; IPR018222; Nuclear_transport_factor_2_euk.
DR PANTHER; PTHR12612; PTHR12612; 1.
DR Pfam; PF02136; NTF2; 1.
DR SUPFAM; SSF54427; SSF54427; 1.
DR PROSITE; PS50177; NTF2_DOMAIN; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cytoplasm; Membrane; mRNA transport; Nuclear pore complex;
KW Nucleus; Protein transport; Reference proteome; Translocation; Transport.
FT CHAIN 1..127
FT /note="Nuclear transport factor 2"
FT /id="PRO_0000194776"
FT DOMAIN 10..121
FT /note="NTF2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00137"
FT MOD_RES 4
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:P61970"
SQ SEQUENCE 127 AA; 14478 MW; 817752F20E262FD3 CRC64;
MGDKPIWEQI GSSFIQHYYQ LFDNDRTQLG AIYIDASCLT WEGQQFQGKA AIVEKLSSLP
FQKIQHSITA QDHQPTPDSC IISMVVGQLK ADEDPIMGFH QMFLLKNIND AWVCTNDMFR
LALHNFG
