NTF2_YEAST
ID NTF2_YEAST Reviewed; 125 AA.
AC P33331; D3DLQ6;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 2.
DT 03-AUG-2022, entry version 168.
DE RecName: Full=Nuclear transport factor 2;
DE Short=NTF-2;
DE AltName: Full=Nuclear transport factor P10;
GN Name=NTF2; OrderedLocusNames=YER009W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169868;
RA Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E.,
RA Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E.,
RA Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S.,
RA Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D.,
RA Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y.,
RA Botstein D., Davis R.W.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
RL Nature 387:78-81(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-84.
RX PubMed=8889515; DOI=10.1093/genetics/144.2.495;
RA Haarer B.K., Corbett A., Kweon Y., Petzold A.S., Silver P., Brown S.S.;
RT "SEC3 mutations are synthetically lethal with profilin mutations and cause
RT defects in diploid-specific bud-site selection.";
RL Genetics 144:495-510(1996).
RN [5]
RP CHARACTERIZATION, AND FUNCTION.
RX PubMed=8600522; DOI=10.1126/science.272.5258.120;
RA Nehrbass U., Blobel G.;
RT "Role of the nuclear transport factor p10 in nuclear import.";
RL Science 272:120-122(1996).
RN [6]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN [7]
RP UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-53, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22106047; DOI=10.1002/pmic.201100166;
RA Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.;
RT "Sites of ubiquitin attachment in Saccharomyces cerevisiae.";
RL Proteomics 12:236-240(2012).
RN [8]
RP X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS), AND SUBCELLULAR LOCATION.
RX PubMed=12065398; DOI=10.1093/emboj/cdf305;
RA Bayliss R., Leung S.W., Baker R.P., Quimby B.B., Corbett A.H., Stewart M.;
RT "Structural basis for the interaction between NTF2 and nucleoporin FxFG
RT repeats.";
RL EMBO J. 21:2843-2853(2002).
CC -!- FUNCTION: Facilitates protein transport into the nucleus. Interacts
CC with various nucleoporins and with Ran-GDP. Could be part of a
CC multicomponent system of cytosolic factors that assemble at the pore
CC complex during nuclear import. In vitro, the NTF2-Ran-GDP association,
CC in the presence of GTP, triggers dissociation of the karyopherin alpha-
CC beta complex, allowing nuclear translocation of karyopherin alpha and
CC the NLS substrate. {ECO:0000269|PubMed:8600522}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:12065398}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U18778; AAB64542.1; -; Genomic_DNA.
DR EMBL; AY558448; AAS56774.1; -; Genomic_DNA.
DR EMBL; L22204; AAB49379.1; -; Genomic_DNA.
DR EMBL; BK006939; DAA07660.1; -; Genomic_DNA.
DR PIR; S50467; S50467.
DR RefSeq; NP_010925.1; NM_001178900.1.
DR PDB; 1GY7; X-ray; 1.60 A; A/B/C/D=1-125.
DR PDB; 1GYB; X-ray; 1.90 A; A/B/C/D=1-125.
DR PDBsum; 1GY7; -.
DR PDBsum; 1GYB; -.
DR AlphaFoldDB; P33331; -.
DR SMR; P33331; -.
DR BioGRID; 36740; 405.
DR DIP; DIP-2245N; -.
DR IntAct; P33331; 2.
DR MINT; P33331; -.
DR STRING; 4932.YER009W; -.
DR iPTMnet; P33331; -.
DR MaxQB; P33331; -.
DR PaxDb; P33331; -.
DR PRIDE; P33331; -.
DR TopDownProteomics; P33331; -.
DR EnsemblFungi; YER009W_mRNA; YER009W; YER009W.
DR GeneID; 856727; -.
DR KEGG; sce:YER009W; -.
DR SGD; S000000811; NTF2.
DR VEuPathDB; FungiDB:YER009W; -.
DR eggNOG; KOG2104; Eukaryota.
DR GeneTree; ENSGT00510000047030; -.
DR HOGENOM; CLU_131642_0_0_1; -.
DR InParanoid; P33331; -.
DR OMA; FTEFYYN; -.
DR BioCyc; YEAST:G3O-30196-MON; -.
DR ChiTaRS; NTF2; yeast.
DR EvolutionaryTrace; P33331; -.
DR PRO; PR:P33331; -.
DR Proteomes; UP000002311; Chromosome V.
DR RNAct; P33331; protein.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005635; C:nuclear envelope; IDA:SGD.
DR GO; GO:0044613; C:nuclear pore central transport channel; IBA:GO_Central.
DR GO; GO:0031267; F:small GTPase binding; IDA:SGD.
DR GO; GO:0006913; P:nucleocytoplasmic transport; IMP:SGD.
DR GO; GO:0006606; P:protein import into nucleus; IMP:SGD.
DR CDD; cd00780; NTF2; 1.
DR InterPro; IPR032710; NTF2-like_dom_sf.
DR InterPro; IPR045875; NTF2/Mtr2.
DR InterPro; IPR002075; NTF2_dom.
DR InterPro; IPR018222; Nuclear_transport_factor_2_euk.
DR PANTHER; PTHR12612; PTHR12612; 1.
DR Pfam; PF02136; NTF2; 1.
DR SUPFAM; SSF54427; SSF54427; 1.
DR PROSITE; PS50177; NTF2_DOMAIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Cytoplasm; Isopeptide bond; Protein transport;
KW Reference proteome; Transport; Ubl conjugation.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22814378"
FT CHAIN 2..125
FT /note="Nuclear transport factor 2"
FT /id="PRO_0000194792"
FT DOMAIN 8..121
FT /note="NTF2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00137"
FT MOD_RES 2
FT /note="N-acetylserine"
FT /evidence="ECO:0007744|PubMed:22814378"
FT CROSSLNK 53
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0007744|PubMed:22106047"
FT HELIX 5..23
FT /evidence="ECO:0007829|PDB:1GY7"
FT HELIX 24..30
FT /evidence="ECO:0007829|PDB:1GY7"
FT STRAND 31..39
FT /evidence="ECO:0007829|PDB:1GY7"
FT STRAND 42..46
FT /evidence="ECO:0007829|PDB:1GY7"
FT HELIX 47..56
FT /evidence="ECO:0007829|PDB:1GY7"
FT STRAND 62..75
FT /evidence="ECO:0007829|PDB:1GY7"
FT STRAND 80..90
FT /evidence="ECO:0007829|PDB:1GY7"
FT STRAND 97..108
FT /evidence="ECO:0007829|PDB:1GY7"
FT STRAND 111..122
FT /evidence="ECO:0007829|PDB:1GY7"
SQ SEQUENCE 125 AA; 14453 MW; 5660D8C00E634714 CRC64;
MSLDFNTLAQ NFTQFYYNQF DTDRSQLGNL YRNESMLTFE TSQLQGAKDI VEKLVSLPFQ
KVQHRITTLD AQPASPNGDV LVMITGDLLI DEEQNPQRFS QVFHLIPDGN SYYVFNDIFR
LNYSA
