NTP1_CAEEL
ID NTP1_CAEEL Reviewed; 543 AA.
AC Q18411; W6RRY0; W6RTH8;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 13-APR-2016, sequence version 2.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Nucleoside-triphosphatase ntp-1;
DE EC=3.6.1.15;
GN Name=ntp-1 {ECO:0000312|WormBase:C33H5.14b};
GN ORFNames=C33H5.14 {ECO:0000312|WormBase:C33H5.14b};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP CATALYTIC ACTIVITY, AND SUBCELLULAR LOCATION.
RX PubMed=15102851; DOI=10.1074/jbc.m402624200;
RA Uccelletti D., O'Callaghan C., Berninsone P., Zemtseva I., Abeijon C.,
RA Hirschberg C.B.;
RT "ire-1-dependent transcriptional up-regulation of a lumenal uridine
RT diphosphatase from Caenorhabditis elegans.";
RL J. Biol. Chem. 279:27390-27398(2004).
CC -!- FUNCTION: Seems to be able to hydrolyze CTP, ATP and UTP.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-
CC diphosphate + H(+) + phosphate; Xref=Rhea:RHEA:23680,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57930, ChEBI:CHEBI:61557; EC=3.6.1.15;
CC Evidence={ECO:0000269|PubMed:15102851};
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000305}; Multi-
CC pass membrane protein {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=b {ECO:0000312|WormBase:C33H5.14b};
CC IsoId=Q18411-1; Sequence=Displayed;
CC Name=a {ECO:0000312|WormBase:C33H5.14a};
CC IsoId=Q18411-2; Sequence=VSP_058210;
CC -!- MISCELLANEOUS: In contrast to uda-1, expression is not induced by
CC stress.
CC -!- SIMILARITY: Belongs to the GDA1/CD39 NTPase family. {ECO:0000305}.
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DR EMBL; BX284604; CDM63521.1; -; Genomic_DNA.
DR EMBL; BX284604; CDM63522.1; -; Genomic_DNA.
DR PIR; T34147; T34147.
DR RefSeq; NP_001294166.1; NM_001307237.1. [Q18411-2]
DR RefSeq; NP_001294167.1; NM_001307238.1. [Q18411-1]
DR AlphaFoldDB; Q18411; -.
DR SMR; Q18411; -.
DR STRING; 6239.C33H5.14.1; -.
DR EPD; Q18411; -.
DR PaxDb; Q18411; -.
DR EnsemblMetazoa; C33H5.14a.1; C33H5.14a.1; WBGene00016380. [Q18411-2]
DR EnsemblMetazoa; C33H5.14b.1; C33H5.14b.1; WBGene00016380. [Q18411-1]
DR GeneID; 177567; -.
DR KEGG; cel:CELE_C33H5.14; -.
DR UCSC; C33H5.14.1; c. elegans. [Q18411-1]
DR CTD; 177567; -.
DR WormBase; C33H5.14a; CE49527; WBGene00016380; ntp-1. [Q18411-2]
DR WormBase; C33H5.14b; CE49492; WBGene00016380; ntp-1. [Q18411-1]
DR eggNOG; KOG1386; Eukaryota.
DR GeneTree; ENSGT01050000244974; -.
DR HOGENOM; CLU_010246_6_1_1; -.
DR InParanoid; Q18411; -.
DR OMA; WYTTSNF; -.
DR OrthoDB; 1337265at2759; -.
DR Reactome; R-CEL-8850843; Phosphate bond hydrolysis by NTPDase proteins.
DR PRO; PR:Q18411; -.
DR Proteomes; UP000001940; Chromosome IV.
DR Bgee; WBGene00016380; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0005794; C:Golgi apparatus; IDA:WormBase.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IDA:WormBase.
DR GO; GO:0016887; F:ATP hydrolysis activity; IDA:WormBase.
DR GO; GO:0043273; F:CTPase activity; IDA:WormBase.
DR GO; GO:0003924; F:GTPase activity; IDA:WormBase.
DR GO; GO:0004382; F:guanosine-diphosphatase activity; IDA:WormBase.
DR GO; GO:0017110; F:nucleoside-diphosphatase activity; IBA:GO_Central.
DR GO; GO:0045134; F:uridine-diphosphatase activity; IDA:WormBase.
DR GO; GO:0046034; P:ATP metabolic process; IDA:WormBase.
DR GO; GO:0046036; P:CTP metabolic process; IDA:WormBase.
DR GO; GO:0046710; P:GDP metabolic process; IDA:WormBase.
DR GO; GO:0046039; P:GTP metabolic process; IDA:WormBase.
DR GO; GO:0009134; P:nucleoside diphosphate catabolic process; IBA:GO_Central.
DR GO; GO:0006256; P:UDP catabolic process; IBA:GO_Central.
DR GO; GO:0046048; P:UDP metabolic process; IDA:WormBase.
DR InterPro; IPR000407; GDA1_CD39_NTPase.
DR PANTHER; PTHR11782; PTHR11782; 1.
DR Pfam; PF01150; GDA1_CD39; 1.
DR PROSITE; PS01238; GDA1_CD39_NTPASE; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Golgi apparatus; Hydrolase; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..543
FT /note="Nucleoside-triphosphatase ntp-1"
FT /id="PRO_0000209924"
FT TRANSMEM 40..60
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 497..517
FT /note="Helical"
FT /evidence="ECO:0000255"
FT ACT_SITE 212
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT VAR_SEQ 1..34
FT /note="Missing (in isoform a)"
FT /evidence="ECO:0000305"
FT /id="VSP_058210"
SQ SEQUENCE 543 AA; 61239 MW; 5119A30BAEF22118 CRC64;
MRWRYTIRDQ SPFEESSNCH RLTSSETANT FRWPMRTYNV YGFLLTCTCL LLILTIIPMS
GGSSERVQRS VRSVVETKNN IKYGVICDAG SSGTRLFVYT LKPLSGGLTN IDTLIHESEP
VVKKVTPGLS SFGDKPEQVV EYLTPLLRFA EEHIPYEQLG ETDLLIFATA GMRLLPEAQK
DAIIKNLQNG LKSVTALRVS DSNIRIIDGA WEGIYSWIAV NYILGRFDKE NDSKVGMIDM
GGASVQIAFE IANEKESYNG GNVYEINLGS IETNEDYKYK IYSTTFLGYG ANEGLKKYEN
SLVKSGNSND SCSPRGLNRL IGEFTVNGTG EWDVCLAQVS SLIGDKAQPS CPNPTCFLRN
VIAPSVNLST VQLYGFSEYW YTTSNFGSGG EYHYQKFTDE VRKYCQKDWN DIQDGFKRNE
FPNADIERLG TNCFKAAWVT SVLHDGFNVD KTKHLFQSVL KIAGEEMQWA LGAMLYHSKD
LKFNLLEQLE VAQSTQQISN FFSFFVILII VLAVALYRQL QSESTYKKYN FLRTDSKPDF
LNV
