NTPA_ENTHA
ID NTPA_ENTHA Reviewed; 593 AA.
AC Q08636; I6TB93;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=V-type sodium ATPase catalytic subunit A;
DE EC=7.2.2.1;
DE AltName: Full=Na(+)-translocating ATPase subunit A;
DE AltName: Full=V-type sodium pump catalytic subunit A;
GN Name=ntpA; OrderedLocusNames=EHR_08260;
OS Enterococcus hirae (strain ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 /
OS NBRC 3181 / NCIMB 6459 / NCDO 1258 / NCTC 12367 / WDCM 00089 / R).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
OC Enterococcus.
OX NCBI_TaxID=768486;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-20; 345-359
RP AND 381-407.
RC STRAIN=ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459
RC / NCDO 1258 / NCTC 12367 / WDCM 00089 / R;
RX PubMed=8505293; DOI=10.1016/s0021-9258(19)50245-9;
RA Takase K., Yamato I., Kakinuma Y.;
RT "Cloning and sequencing of the genes coding for the A and B subunits of
RT vacuolar-type Na(+)-ATPase from Enterococcus hirae. Coexistence of
RT vacuolar- and F0F1-type ATPases in one bacterial cell.";
RL J. Biol. Chem. 268:11610-11616(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459
RC / NCDO 1258 / NCTC 12367 / WDCM 00089 / R;
RX PubMed=22933757; DOI=10.1128/jb.01075-12;
RA Gaechter T., Wunderlin C., Schmidheini T., Solioz M.;
RT "Genome sequence of Enterococcus hirae (Streptococcus faecalis) ATCC 9790,
RT a model organism for the study of ion transport, bioenergetics, and copper
RT homeostasis.";
RL J. Bacteriol. 194:5126-5127(2012).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-187.
RC STRAIN=ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459
RC / NCDO 1258 / NCTC 12367 / WDCM 00089 / R;
RX PubMed=8144530; DOI=10.1016/s0021-9258(17)36902-8;
RA Solioz M., Davies K.;
RT "Operon of vacuolar-type Na(+)-ATPase of Enterococcus hirae.";
RL J. Biol. Chem. 269:9453-9459(1994).
CC -!- FUNCTION: Involved in ATP-driven sodium extrusion.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + 4 Na(+)(in) = ADP + H(+) + 4 Na(+)(out) +
CC phosphate; Xref=Rhea:RHEA:58156, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:29101, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=7.2.2.1;
CC -!- INTERACTION:
CC Q08636; Q08637: ntpB; NbExp=3; IntAct=EBI-16032906, EBI-16032937;
CC -!- INDUCTION: By increasing intracellular Na(+) concentration.
CC -!- SIMILARITY: Belongs to the ATPase alpha/beta chains family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; D13816; BAA02969.1; -; Genomic_DNA.
DR EMBL; D17462; BAA04275.1; -; Genomic_DNA.
DR EMBL; CP003504; AFM70579.1; -; Genomic_DNA.
DR EMBL; X76913; CAA54241.1; -; Genomic_DNA.
DR PIR; A46733; A46733.
DR RefSeq; WP_010718637.1; NZ_KB946231.1.
DR PDB; 3VR2; X-ray; 2.80 A; A/B/C=1-593.
DR PDB; 3VR3; X-ray; 3.40 A; A/B/C=1-593.
DR PDB; 3VR4; X-ray; 2.17 A; A/B/C=1-593.
DR PDB; 3VR5; X-ray; 3.90 A; A/B/C=1-593.
DR PDB; 3VR6; X-ray; 2.68 A; A/B/C=1-593.
DR PDB; 5KNB; X-ray; 3.25 A; A/B/C=1-593.
DR PDB; 5KNC; X-ray; 3.02 A; A/B/C=1-593.
DR PDB; 5KND; X-ray; 2.89 A; A/B/C=1-593.
DR PDB; 5ZE9; X-ray; 2.10 A; A/B/C=1-593.
DR PDB; 5ZEA; X-ray; 3.38 A; A/B/C/G/H/I=1-587.
DR PDB; 7COP; X-ray; 2.77 A; A/B/C=1-593.
DR PDB; 7COQ; X-ray; 3.44 A; A/B/C=1-593.
DR PDB; 7COR; X-ray; 2.90 A; A/B/C=1-593.
DR PDB; 7COS; X-ray; 3.95 A; A/B/C/G/H/I=1-593.
DR PDB; 7DQC; X-ray; 2.71 A; A/B/C=1-593.
DR PDB; 7DQD; X-ray; 3.38 A; A/B/C/I/J/K=1-593.
DR PDB; 7DQE; X-ray; 2.69 A; A/B/C=1-593.
DR PDBsum; 3VR2; -.
DR PDBsum; 3VR3; -.
DR PDBsum; 3VR4; -.
DR PDBsum; 3VR5; -.
DR PDBsum; 3VR6; -.
DR PDBsum; 5KNB; -.
DR PDBsum; 5KNC; -.
DR PDBsum; 5KND; -.
DR PDBsum; 5ZE9; -.
DR PDBsum; 5ZEA; -.
DR PDBsum; 7COP; -.
DR PDBsum; 7COQ; -.
DR PDBsum; 7COR; -.
DR PDBsum; 7COS; -.
DR PDBsum; 7DQC; -.
DR PDBsum; 7DQD; -.
DR PDBsum; 7DQE; -.
DR AlphaFoldDB; Q08636; -.
DR SMR; Q08636; -.
DR DIP; DIP-60125N; -.
DR IntAct; Q08636; 3.
DR STRING; 768486.EHR_08260; -.
DR TCDB; 3.A.2.2.2; the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.
DR EnsemblBacteria; AFM70579; AFM70579; EHR_08260.
DR KEGG; ehr:EHR_08260; -.
DR eggNOG; COG1155; Bacteria.
DR HOGENOM; CLU_008162_3_1_9; -.
DR OrthoDB; 875807at2; -.
DR BioCyc; MetaCyc:MON-14146; -.
DR Proteomes; UP000002895; Chromosome.
DR GO; GO:0045259; C:proton-transporting ATP synthase complex; IEA:UniProt.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0046933; F:proton-transporting ATP synthase activity, rotational mechanism; IEA:UniProtKB-UniRule.
DR GO; GO:0046961; F:proton-transporting ATPase activity, rotational mechanism; IEA:InterPro.
DR GO; GO:0046932; F:sodium-transporting ATP synthase activity, rotational mechanism; IEA:UniProtKB-EC.
DR GO; GO:0046962; F:sodium-transporting ATPase activity, rotational mechanism; IEA:UniProtKB-EC.
DR GO; GO:0042777; P:proton motive force-driven plasma membrane ATP synthesis; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.1140.10; -; 1.
DR Gene3D; 2.40.30.20; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00309; ATP_synth_A_arch; 1.
DR InterPro; IPR031686; ATP-synth_a_Xtn.
DR InterPro; IPR023366; ATP_synth_asu-like_sf.
DR InterPro; IPR020003; ATPase_a/bsu_AS.
DR InterPro; IPR004100; ATPase_F1/V1/A1_a/bsu_N.
DR InterPro; IPR036121; ATPase_F1/V1/A1_a/bsu_N_sf.
DR InterPro; IPR000194; ATPase_F1/V1/A1_a/bsu_nucl-bd.
DR InterPro; IPR024034; ATPase_F1/V1_b/a_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR022878; V-ATPase_asu.
DR PANTHER; PTHR43607; PTHR43607; 1.
DR Pfam; PF00006; ATP-synt_ab; 1.
DR Pfam; PF02874; ATP-synt_ab_N; 1.
DR Pfam; PF16886; ATP-synt_ab_Xtn; 1.
DR SUPFAM; SSF50615; SSF50615; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00152; ATPASE_ALPHA_BETA; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Direct protein sequencing; Ion transport;
KW Nucleotide-binding; Sodium; Sodium transport; Translocase; Transport.
FT CHAIN 1..593
FT /note="V-type sodium ATPase catalytic subunit A"
FT /id="PRO_0000144622"
FT BINDING 232..239
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT STRAND 4..10
FT /evidence="ECO:0007829|PDB:5ZE9"
FT STRAND 13..18
FT /evidence="ECO:0007829|PDB:5ZE9"
FT STRAND 26..30
FT /evidence="ECO:0007829|PDB:5ZE9"
FT TURN 31..34
FT /evidence="ECO:0007829|PDB:5ZE9"
FT STRAND 35..43
FT /evidence="ECO:0007829|PDB:5ZE9"
FT STRAND 46..53
FT /evidence="ECO:0007829|PDB:5ZE9"
FT STRAND 63..76
FT /evidence="ECO:0007829|PDB:5ZE9"
FT STRAND 82..84
FT /evidence="ECO:0007829|PDB:7DQD"
FT HELIX 91..98
FT /evidence="ECO:0007829|PDB:5ZE9"
FT STRAND 100..102
FT /evidence="ECO:0007829|PDB:3VR4"
FT STRAND 118..122
FT /evidence="ECO:0007829|PDB:5ZE9"
FT STRAND 129..131
FT /evidence="ECO:0007829|PDB:7DQC"
FT STRAND 135..144
FT /evidence="ECO:0007829|PDB:5ZE9"
FT STRAND 146..150
FT /evidence="ECO:0007829|PDB:5ZE9"
FT STRAND 157..162
FT /evidence="ECO:0007829|PDB:5ZE9"
FT STRAND 165..167
FT /evidence="ECO:0007829|PDB:5ZE9"
FT STRAND 169..171
FT /evidence="ECO:0007829|PDB:7DQE"
FT STRAND 174..177
FT /evidence="ECO:0007829|PDB:5ZE9"
FT STRAND 182..185
FT /evidence="ECO:0007829|PDB:5ZE9"
FT STRAND 189..192
FT /evidence="ECO:0007829|PDB:5ZE9"
FT STRAND 200..203
FT /evidence="ECO:0007829|PDB:5ZE9"
FT HELIX 214..219
FT /evidence="ECO:0007829|PDB:5ZE9"
FT STRAND 227..231
FT /evidence="ECO:0007829|PDB:5ZE9"
FT HELIX 238..248
FT /evidence="ECO:0007829|PDB:5ZE9"
FT STRAND 252..260
FT /evidence="ECO:0007829|PDB:5ZE9"
FT HELIX 263..272
FT /evidence="ECO:0007829|PDB:5ZE9"
FT HELIX 273..275
FT /evidence="ECO:0007829|PDB:5ZE9"
FT TURN 279..281
FT /evidence="ECO:0007829|PDB:5ZE9"
FT STRAND 282..284
FT /evidence="ECO:0007829|PDB:3VR2"
FT HELIX 285..288
FT /evidence="ECO:0007829|PDB:5ZE9"
FT STRAND 289..294
FT /evidence="ECO:0007829|PDB:5ZE9"
FT HELIX 301..319
FT /evidence="ECO:0007829|PDB:5ZE9"
FT STRAND 323..329
FT /evidence="ECO:0007829|PDB:5ZE9"
FT HELIX 331..344
FT /evidence="ECO:0007829|PDB:5ZE9"
FT HELIX 351..353
FT /evidence="ECO:0007829|PDB:5ZE9"
FT HELIX 358..367
FT /evidence="ECO:0007829|PDB:5ZE9"
FT STRAND 370..373
FT /evidence="ECO:0007829|PDB:5ZE9"
FT STRAND 375..378
FT /evidence="ECO:0007829|PDB:7DQE"
FT STRAND 381..390
FT /evidence="ECO:0007829|PDB:5ZE9"
FT HELIX 393..395
FT /evidence="ECO:0007829|PDB:5ZE9"
FT STRAND 397..399
FT /evidence="ECO:0007829|PDB:7COP"
FT HELIX 400..406
FT /evidence="ECO:0007829|PDB:5ZE9"
FT STRAND 410..412
FT /evidence="ECO:0007829|PDB:5ZE9"
FT HELIX 417..421
FT /evidence="ECO:0007829|PDB:5ZE9"
FT TURN 430..432
FT /evidence="ECO:0007829|PDB:5ZE9"
FT HELIX 438..449
FT /evidence="ECO:0007829|PDB:5ZE9"
FT HELIX 453..477
FT /evidence="ECO:0007829|PDB:5ZE9"
FT HELIX 479..481
FT /evidence="ECO:0007829|PDB:5ZE9"
FT HELIX 484..499
FT /evidence="ECO:0007829|PDB:5ZE9"
FT STRAND 505..507
FT /evidence="ECO:0007829|PDB:7DQE"
FT TURN 508..511
FT /evidence="ECO:0007829|PDB:5ZE9"
FT HELIX 515..537
FT /evidence="ECO:0007829|PDB:5ZE9"
FT STRAND 538..540
FT /evidence="ECO:0007829|PDB:7DQD"
FT HELIX 542..547
FT /evidence="ECO:0007829|PDB:5ZE9"
FT HELIX 550..558
FT /evidence="ECO:0007829|PDB:5ZE9"
FT HELIX 559..561
FT /evidence="ECO:0007829|PDB:5ZE9"
FT HELIX 564..567
FT /evidence="ECO:0007829|PDB:5ZE9"
FT HELIX 568..571
FT /evidence="ECO:0007829|PDB:5ZE9"
FT HELIX 573..586
FT /evidence="ECO:0007829|PDB:5ZE9"
SQ SEQUENCE 593 AA; 65770 MW; 3E209C2D8687B48A CRC64;
MQIGKIIKVS GPLVMAENMS EASIQDMCLV GDLGVIGEII EMRQDVASIQ VYEETSGIGP
GEPVRSTGEA LSVELGPGII SQMFDGIQRP LDTFMEVTQS NFLGRGVQLP ALDHEKQWWF
EATIEEGTEV SAGDIIGYVD ETKIIQHKIM VPNGIKGTVQ KIESGSFTID DPICVIETEQ
GLKELTMMQK WPVRRGRPIK QKLNPDVPMI TGQRVIDTFF PVTKGGAAAV PGPFGAGKTV
VQHQIAKWSD VDLVVYVGCG ERGNEMTDVV NEFPELIDPN TGESLMERTV LIANTSNMPV
AAREASIYTG ITIAEYFRDM GYDVAIMADS TSRWAEALRE MSGRLEEMPG DEGYPAYLGS
RLAEYYERSG RVIALGSDQR EGSITAISAV SPSGGDISEP VTQNTLRVVK VFWGLDSSLA
QKRHFPSINW IQSYSLYSTE VGRYMDQILQ QDWSDMVTEG MRILQEEEQL NEIVRLVGID
SLSDNDRLTL EVAKSIREDY LQQNAFDDVD TFTSREKQFN MLKVILTFGK EARKALSLGA
YFNEIMEGTV AVRERISRSK YIPEEELAKI SSINEEIKET IQLIVSEGGM TDD
