NTPJ_ENTHA
ID NTPJ_ENTHA Reviewed; 451 AA.
AC P43440; I6SYN3;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2013, sequence version 2.
DT 25-MAY-2022, entry version 107.
DE RecName: Full=Potassium/sodium uptake protein NtpJ;
DE AltName: Full=K(+):Na(+) symporter;
GN Name=ntpJ; OrderedLocusNames=EHR_08275;
OS Enterococcus hirae (strain ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 /
OS NBRC 3181 / NCIMB 6459 / NCDO 1258 / NCTC 12367 / WDCM 00089 / R).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
OC Enterococcus.
OX NCBI_TaxID=768486;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459
RC / NCDO 1258 / NCTC 12367 / WDCM 00089 / R;
RX PubMed=8157629; DOI=10.1016/s0021-9258(19)78088-0;
RA Takase K., Kakinuma S., Yamato I., Konishi K., Igarashi K., Kakinuma Y.;
RT "Sequencing and characterization of the ntp gene cluster for vacuolar-type
RT Na(+)-translocating ATPase of Enterococcus hirae.";
RL J. Biol. Chem. 269:11037-11044(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459
RC / NCDO 1258 / NCTC 12367 / WDCM 00089 / R;
RX PubMed=22933757; DOI=10.1128/jb.01075-12;
RA Gaechter T., Wunderlin C., Schmidheini T., Solioz M.;
RT "Genome sequence of Enterococcus hirae (Streptococcus faecalis) ATCC 9790,
RT a model organism for the study of ion transport, bioenergetics, and copper
RT homeostasis.";
RL J. Bacteriol. 194:5126-5127(2012).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459
RC / NCDO 1258 / NCTC 12367 / WDCM 00089 / R;
RX PubMed=8626559; DOI=10.1074/jbc.271.17.10042;
RA Murata T., Takase K., Yamato I., Igarashi K., Kakinuma Y.;
RT "The ntpJ gene in the Enterococcus hirae ntp operon encodes a component of
RT KtrII potassium transport system functionally independent of vacuolar Na+-
RT ATPase.";
RL J. Biol. Chem. 271:10042-10047(1996).
RN [4]
RP FUNCTION AS A POTASSIUM AND SODIUM TRANSPORTER.
RC STRAIN=ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459
RC / NCDO 1258 / NCTC 12367 / WDCM 00089 / R;
RX PubMed=10762252; DOI=10.1128/jb.182.9.2507-2512.2000;
RA Kawano M., Abuki R., Igarashi K., Kakinuma Y.;
RT "Evidence for Na(+) influx via the NtpJ protein of the KtrII K(+) uptake
RT system in Enterococcus hirae.";
RL J. Bacteriol. 182:2507-2512(2000).
CC -!- FUNCTION: Mediates electrogenic transport of potassium as well as
CC sodium. Acts probably as a potassium-sodium cotransporter. Major sodium
CC reentry pathway at high pH values. {ECO:0000269|PubMed:10762252,
CC ECO:0000269|PubMed:8626559}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Mutants are defective in potassium accumulation
CC at high pH and lack KtrII activity. Disruption does not affect Na(+)-
CC ATPase activity. {ECO:0000269|PubMed:8626559}.
CC -!- SIMILARITY: Belongs to the TrkH potassium transport family.
CC {ECO:0000305}.
CC -!- CAUTION: Was originally thought to be part of the V-type sodium ATP
CC synthase. {ECO:0000305|PubMed:8157629}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AFM70582.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; D17462; BAA04279.1; -; Genomic_DNA.
DR EMBL; CP003504; AFM70582.1; ALT_INIT; Genomic_DNA.
DR PIR; G53610; G53610.
DR AlphaFoldDB; P43440; -.
DR SMR; P43440; -.
DR STRING; 768486.EHR_08275; -.
DR TCDB; 2.A.38.4.1; the k(+) transporter (trk) family.
DR EnsemblBacteria; AFM70582; AFM70582; EHR_08275.
DR KEGG; ehr:EHR_08275; -.
DR eggNOG; COG0168; Bacteria.
DR HOGENOM; CLU_026429_0_1_9; -.
DR Proteomes; UP000002895; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015379; F:potassium:chloride symporter activity; IEA:InterPro.
DR GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR InterPro; IPR003445; Cat_transpt.
DR InterPro; IPR004772; TrkH.
DR Pfam; PF02386; TrkH; 1.
DR TIGRFAMs; TIGR00933; 2a38; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Ion transport; Membrane; Potassium; Potassium transport;
KW Sodium; Sodium transport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..451
FT /note="Potassium/sodium uptake protein NtpJ"
FT /id="PRO_0000070480"
FT TRANSMEM 18..38
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 46..66
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 78..98
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..153
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 162..182
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 192..212
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 230..250
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 293..313
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 350..370
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 380..400
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 410..430
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 428
FT /note="F -> L (in Ref. 1; BAA04279)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 451 AA; 49249 MW; 59837234B857E87F CRC64;
MTIMKKRVRK RLSPVQLIAA GFFILILFGG SLLTLPFFSR SGESTHFIDA LFTATSAVCV
TGLTTLNTAE HWNSAGQFLI MTLIEIGGLG FMMIPILFFA IAKKKISFSM RIVLKEALNL
EEMSGVIKLM IYILKFAVVI QVIGAVALSV VFIPEFGWAK GIWFSIFHAV SSFCNAGFDL
LGDSLLADQT NVYLIMVVSA LIIAGGLGFI VWRDILSYHR VKKITLHSKV ALSVTALLLI
GGFILFLITE RNGLTLVKGT FTERLANTFF MSVTPRTAGY YSIDYLQMSH AGLILTMFLM
YIGGTSGSTA GGLKTTTLGI LLIQMHAMFK GKTRAEAFGR TIRQAAVLRA LTLFFVTLSL
CVVAIMVLSV TETIPKTSGI EYIAFEVFSA FGTVGLTMGL TPDLTLIGKL VIISLMYIGR
VGIMTVVFSL LVKANRAEAN YKYPEESIML G
