NTPK_ENTHA
ID NTPK_ENTHA Reviewed; 156 AA.
AC P43457; I6S1L2;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=V-type sodium ATPase subunit K;
DE AltName: Full=Na(+)-translocating ATPase subunit K;
DE AltName: Full=Sodium ATPase proteolipid component;
GN Name=ntpK; Synonyms=ntpN; OrderedLocusNames=EHR_08240;
OS Enterococcus hirae (strain ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 /
OS NBRC 3181 / NCIMB 6459 / NCDO 1258 / NCTC 12367 / WDCM 00089 / R).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
OC Enterococcus.
OX NCBI_TaxID=768486;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 84-95.
RC STRAIN=ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459
RC / NCDO 1258 / NCTC 12367 / WDCM 00089 / R;
RX PubMed=8373385; DOI=10.1006/bbrc.1993.2152;
RA Kakinuma Y., Kakinuma S., Takase K., Konishi K., Igarashi K., Yamato I.;
RT "A gene encoding the 16-kDa proteolipid subunit of Enterococcus hirae
RT Na(+)-ATPase complex.";
RL Biochem. Biophys. Res. Commun. 195:1063-1069(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459
RC / NCDO 1258 / NCTC 12367 / WDCM 00089 / R;
RX PubMed=8157629; DOI=10.1016/s0021-9258(19)78088-0;
RA Takase K., Kakinuma S., Yamato I., Konishi K., Igarashi K., Kakinuma Y.;
RT "Sequencing and characterization of the ntp gene cluster for vacuolar-type
RT Na(+)-translocating ATPase of Enterococcus hirae.";
RL J. Biol. Chem. 269:11037-11044(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459
RC / NCDO 1258 / NCTC 12367 / WDCM 00089 / R;
RX PubMed=8144530; DOI=10.1016/s0021-9258(17)36902-8;
RA Solioz M., Davies K.;
RT "Operon of vacuolar-type Na(+)-ATPase of Enterococcus hirae.";
RL J. Biol. Chem. 269:9453-9459(1994).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459
RC / NCDO 1258 / NCTC 12367 / WDCM 00089 / R;
RX PubMed=22933757; DOI=10.1128/jb.01075-12;
RA Gaechter T., Wunderlin C., Schmidheini T., Solioz M.;
RT "Genome sequence of Enterococcus hirae (Streptococcus faecalis) ATCC 9790,
RT a model organism for the study of ion transport, bioenergetics, and copper
RT homeostasis.";
RL J. Bacteriol. 194:5126-5127(2012).
CC -!- FUNCTION: Involved in ATP-driven sodium extrusion.
CC -!- INTERACTION:
CC P43457; P43457: ntpK; NbExp=3; IntAct=EBI-15709960, EBI-15709960;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- PTM: The N-terminus is blocked.
CC -!- SIMILARITY: Belongs to the V-ATPase proteolipid subunit family.
CC {ECO:0000305}.
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DR EMBL; D16334; BAA03841.1; -; Genomic_DNA.
DR EMBL; D17462; BAA04271.1; -; Genomic_DNA.
DR EMBL; X76913; CAA54237.1; -; Genomic_DNA.
DR EMBL; CP003504; AFM70575.1; -; Genomic_DNA.
DR PIR; C54392; C54392.
DR RefSeq; WP_010738015.1; NZ_KB946231.1.
DR PDB; 2BL2; X-ray; 2.10 A; A/B/C/D/E/F/G/H/I/J=1-156.
DR PDB; 2CYD; X-ray; 2.80 A; A/B/C/D/E/F/G/H/I/J=1-156.
DR PDB; 2DB4; X-ray; 2.40 A; A/B/C/D/E/F/G/H/I/J=1-156.
DR PDB; 3AOU; X-ray; 3.14 A; A/B/C/D/E/F/G/H/I/J=1-156.
DR PDBsum; 2BL2; -.
DR PDBsum; 2CYD; -.
DR PDBsum; 2DB4; -.
DR PDBsum; 3AOU; -.
DR AlphaFoldDB; P43457; -.
DR SMR; P43457; -.
DR DIP; DIP-46127N; -.
DR STRING; 768486.EHR_08240; -.
DR DrugBank; DB02043; 1,2-Dipalmitoyl-Phosphatidyl-Glycerole.
DR DrugBank; DB02686; Undecyl-Beta-D-Maltopyranoside.
DR TCDB; 3.A.2.2.2; the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.
DR EnsemblBacteria; AFM70575; AFM70575; EHR_08240.
DR GeneID; 66464796; -.
DR KEGG; ehr:EHR_08240; -.
DR eggNOG; COG0636; Bacteria.
DR HOGENOM; CLU_126047_0_0_9; -.
DR OrthoDB; 1950613at2; -.
DR BioCyc; MetaCyc:MON-14153; -.
DR EvolutionaryTrace; P43457; -.
DR Proteomes; UP000002895; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0033179; C:proton-transporting V-type ATPase, V0 domain; IEA:InterPro.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0046961; F:proton-transporting ATPase activity, rotational mechanism; IEA:InterPro.
DR GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR Gene3D; 1.20.120.610; -; 1.
DR InterPro; IPR002379; ATPase_proteolipid_c-like_dom.
DR InterPro; IPR000245; ATPase_proteolipid_csu.
DR InterPro; IPR035921; F/V-ATP_Csub_sf.
DR Pfam; PF00137; ATP-synt_C; 2.
DR PRINTS; PR00122; VACATPASE.
DR SUPFAM; SSF81333; SSF81333; 2.
PE 1: Evidence at protein level;
KW 3D-structure; Cell membrane; Direct protein sequencing; Ion transport;
KW Membrane; Sodium; Sodium transport; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..156
FT /note="V-type sodium ATPase subunit K"
FT /id="PRO_0000071781"
FT TRANSMEM 11..31
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 60..80
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 89..109
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 132..152
FT /note="Helical"
FT /evidence="ECO:0000255"
FT HELIX 2..7
FT /evidence="ECO:0007829|PDB:2BL2"
FT TURN 8..11
FT /evidence="ECO:0007829|PDB:2DB4"
FT HELIX 12..45
FT /evidence="ECO:0007829|PDB:2BL2"
FT HELIX 49..51
FT /evidence="ECO:0007829|PDB:2BL2"
FT HELIX 52..60
FT /evidence="ECO:0007829|PDB:2BL2"
FT HELIX 61..63
FT /evidence="ECO:0007829|PDB:2BL2"
FT HELIX 64..78
FT /evidence="ECO:0007829|PDB:2BL2"
FT STRAND 82..84
FT /evidence="ECO:0007829|PDB:3AOU"
FT HELIX 86..123
FT /evidence="ECO:0007829|PDB:2BL2"
FT HELIX 125..127
FT /evidence="ECO:0007829|PDB:2BL2"
FT HELIX 128..136
FT /evidence="ECO:0007829|PDB:2BL2"
FT HELIX 139..155
FT /evidence="ECO:0007829|PDB:2BL2"
SQ SEQUENCE 156 AA; 16037 MW; 650D7F4633EB2E0B CRC64;
MMDYLITQNG GMVFAVLAMA TATIFSGIGS AKGVGMTGEA AAALTTSQPE KFGQALILQL
LPGTQGLYGF VIAFLIFINL GSDMSVVQGL NFLGASLPIA FTGLFSGIAQ GKVAAAGIQI
LAKKPEHATK GIIFAAMVET YAILGFVISF LLVLNA
