NTR2_CAEEL
ID NTR2_CAEEL Reviewed; 398 AA.
AC O62169;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Nematocin receptor 2 {ECO:0000303|PubMed:23112336};
GN Name=ntr-2 {ECO:0000303|PubMed:23112336};
GN ORFNames=F14F4.1 {ECO:0000312|WormBase:F14F4.1};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|EMBL:AFJ42490.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RX PubMed=23112336; DOI=10.1126/science.1226860;
RA Beets I., Janssen T., Meelkop E., Temmerman L., Suetens N., Rademakers S.,
RA Jansen G., Schoofs L.;
RT "Vasopressin/oxytocin-related signaling regulates gustatory associative
RT learning in C. elegans.";
RL Science 338:543-545(2012).
RN [2] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3] {ECO:0000305}
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=23112335; DOI=10.1126/science.1226201;
RA Garrison J.L., Macosko E.Z., Bernstein S., Pokala N., Albrecht D.R.,
RA Bargmann C.I.;
RT "Oxytocin/vasopressin-related peptides have an ancient role in reproductive
RT behavior.";
RL Science 338:540-543(2012).
CC -!- FUNCTION: Not directly activated by nematocin (PubMed:23112336,
CC PubMed:23112335). May modulate activity of the nematocin receptor ntr-
CC 1, leading to reduced intracellular cAMP production (PubMed:23112335).
CC Plays a role in male mating behavior (PubMed:23112335).
CC {ECO:0000269|PubMed:23112335, ECO:0000269|PubMed:23112336}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:23112336};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Detected in the ADL sensory neurons, the RMED and
CC RMEV motor neurons, and the PQR tail neuron (PubMed:23112336). In
CC males, detected in SPC tail neurons involved in spicule penetration and
CC sperm transfer, and male-specific oblique muscles involved in vulval
CC contact (PubMed:23112335). {ECO:0000269|PubMed:23112335,
CC ECO:0000269|PubMed:23112336}.
CC -!- DISRUPTION PHENOTYPE: Viable and fertile. Males have reduced
CC reproductive success, due to a range of aberrant mating behaviors.
CC Double knockouts with ntr-1 partially rescue the reproductive
CC phenotypes. {ECO:0000269|PubMed:23112335}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC Vasopressin/oxytocin receptor subfamily. {ECO:0000305}.
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DR EMBL; JQ277479; AFJ42490.1; -; mRNA.
DR EMBL; BX284606; CAA16265.1; -; Genomic_DNA.
DR PIR; T20901; T20901.
DR RefSeq; NP_510477.1; NM_078076.1.
DR AlphaFoldDB; O62169; -.
DR SMR; O62169; -.
DR STRING; 6239.F14F4.1; -.
DR PaxDb; O62169; -.
DR EnsemblMetazoa; F14F4.1.1; F14F4.1.1; WBGene00008808.
DR GeneID; 184471; -.
DR KEGG; cel:CELE_F14F4.1; -.
DR UCSC; F14F4.1; c. elegans.
DR CTD; 184471; -.
DR WormBase; F14F4.1; CE17670; WBGene00008808; ntr-2.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT00970000195859; -.
DR HOGENOM; CLU_009579_15_0_1; -.
DR InParanoid; O62169; -.
DR OMA; RAMDSQK; -.
DR OrthoDB; 890925at2759; -.
DR PhylomeDB; O62169; -.
DR PRO; PR:O62169; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00008808; Expressed in larva and 1 other tissue.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IEA:UniProtKB-KW.
DR GO; GO:0007610; P:behavior; IEA:UniProtKB-KW.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 2: Evidence at transcript level;
KW Behavior; Cell membrane; Disulfide bond; G-protein coupled receptor;
KW Glycoprotein; Membrane; Receptor; Reference proteome; Transducer;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..398
FT /note="Nematocin receptor 2"
FT /id="PRO_0000438125"
FT TOPO_DOM 1..25
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 26..46
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 47..58
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 59..79
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 80..99
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 100..120
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 121..143
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 144..164
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 165..187
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 188..208
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 209..271
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 272..292
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 293..302
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 303..325
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 326..398
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT CARBOHYD 3
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 7
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT DISULFID 98..173
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
SQ SEQUENCE 398 AA; 45991 MW; 41AC4F9E56F3030D CRC64;
MNNNTLNITN QRTAAAMSQI YFLVVYQTAV MIVSLLGNLF LLFVIFRANQ VMKRRVSPVQ
LLIIHTCVAD LLFALLSLGT EILTLRTYPQ YYGSNFVCKL MRYVQMFPMY ASPFLLVAIS
ADRYQAICRP LAHFRSSRYR RPNWMAAIAW GLALVLSIPQ FFVWTKHSKT GRCSTIYGQN
KNTVKITYVI MFNTLAWLLP SILAAVFYYC VCKAVRLSST KSVRAMDSQK RNGKYSSGAT
EDYIEELRKK SKGFRQQMSE FDRKRVQTVR LTITIVACNF FLWMPFCLIN VIQALWPEIS
HIMFINYVAI LGNLNSCLNP WIYILFNRSH VRKALCRSRR SFTEVTKKRS FENFECSSTA
TMNNNYNNCH AYTAFSNRSQ LKFDSYATDS TSLKTNSN
