NTR2_RAT
ID NTR2_RAT Reviewed; 416 AA.
AC Q63384;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Neurotensin receptor type 2;
DE Short=NT-R-2;
DE Short=NTR2;
DE AltName: Full=High-affinity levocabastine-sensitive neurotensin receptor;
GN Name=Ntsr2; Synonyms=Ntr2;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Hypothalamus;
RX PubMed=8647296; DOI=10.1016/0014-5793(96)00397-3;
RA Chalon P., Vita N., Kaghad M., Guillemont M., Bonin J., Delpech B.,
RA le Fur G., Ferrara P., Caput D.;
RT "Molecular cloning of a levocabastine-sensitive neurotensin binding site.";
RL FEBS Lett. 386:91-94(1996).
RN [2]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-410, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: Receptor for the tridecapeptide neurotensin. It is associated
CC with G proteins that activate a phosphatidylinositol-calcium second
CC messenger system.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Abundant in cortex and hypothalamus, and lower
CC levels seen in the heart and intestine.
CC -!- DEVELOPMENTAL STAGE: Expressed maximally in 7-day-old brain and
CC expression decreases progressively until adulthood (35-day-old brain).
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC Neurotensin receptor subfamily. NTSR2 sub-subfamily.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; X97121; CAA65787.1; -; mRNA.
DR PIR; S68822; S68822.
DR RefSeq; NP_073186.1; NM_022695.2.
DR AlphaFoldDB; Q63384; -.
DR SMR; Q63384; -.
DR STRING; 10116.ENSRNOP00000067549; -.
DR BindingDB; Q63384; -.
DR ChEMBL; CHEMBL5106; -.
DR DrugCentral; Q63384; -.
DR GuidetoPHARMACOLOGY; 310; -.
DR iPTMnet; Q63384; -.
DR PhosphoSitePlus; Q63384; -.
DR PaxDb; Q63384; -.
DR PRIDE; Q63384; -.
DR GeneID; 64636; -.
DR KEGG; rno:64636; -.
DR CTD; 23620; -.
DR RGD; 70962; Ntsr2.
DR eggNOG; KOG3656; Eukaryota.
DR InParanoid; Q63384; -.
DR OrthoDB; 890529at2759; -.
DR PhylomeDB; Q63384; -.
DR Reactome; R-RNO-375276; Peptide ligand-binding receptors.
DR Reactome; R-RNO-416476; G alpha (q) signalling events.
DR PRO; PR:Q63384; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0009986; C:cell surface; IDA:RGD.
DR GO; GO:0043198; C:dendritic shaft; IDA:RGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; ISO:RGD.
DR GO; GO:0043204; C:perikaryon; IDA:RGD.
DR GO; GO:0005886; C:plasma membrane; IDA:RGD.
DR GO; GO:0005802; C:trans-Golgi network; IDA:RGD.
DR GO; GO:0016492; F:G protein-coupled neurotensin receptor activity; ISO:RGD.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IBA:GO_Central.
DR GO; GO:0042802; F:identical protein binding; IPI:RGD.
DR GO; GO:0044877; F:protein-containing complex binding; IPI:RGD.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0007218; P:neuropeptide signaling pathway; IBA:GO_Central.
DR GO; GO:0007200; P:phospholipase C-activating G protein-coupled receptor signaling pathway; ISO:RGD.
DR GO; GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; IDA:RGD.
DR GO; GO:0043410; P:positive regulation of MAPK cascade; IDA:RGD.
DR GO; GO:0042391; P:regulation of membrane potential; ISO:RGD.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR003986; NT2_rcpt.
DR InterPro; IPR003984; NT_rcpt.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR01479; NEUROTENSINR.
DR PRINTS; PR01481; NEUROTENSN2R.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Lipoprotein;
KW Membrane; Palmitate; Phosphoprotein; Receptor; Reference proteome;
KW Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..416
FT /note="Neurotensin receptor type 2"
FT /id="PRO_0000069951"
FT TOPO_DOM 1..32
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 33..55
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 56..64
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 65..87
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 88..109
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 110..131
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 132..154
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 155..176
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 177..216
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 217..237
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 238..297
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 298..318
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 319..337
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 338..358
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 359..416
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MOD_RES 410
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT LIPID 377
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000255"
FT DISULFID 108..194
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
SQ SEQUENCE 416 AA; 46266 MW; 127FC5F5CB6FE208 CRC64;
METSSPWPPR PSPSAGLSLE ARLGVDTRLW AKVLFTALYS LIFAFGTAGN ALSVHVVLKA
RAGRPGRLRY HVLSLALSAL LLLLVSMPME LYNFVWSHYP WVFGDLGCRG YYFVRELCAY
ATVLSVASLS AERCLAVCQP LRARRLLTPR RTRRLLSLVW VASLGLALPM AVIMGQKHEV
ESADGEPEPA SRVCTVLVSR ATLQVFIQVN VLVSFALPLA LTAFLNGITV NHLMALYSQV
PSASAQVSSI PSRLELLSEE GLLGFITWRK TLSLGVQASL VRHKDASQIR SLQHSAQVLR
AIVAVYVICW LPYHARRLMY CYIPDDGWTN ELYDFYHYFY MVTNTLFYVS SAVTPILYNA
VSSSFRKLFL ESLGSLCGEQ HSLVPLPQEA PESTTSTYSF RLWGSPRNPS LGEIQV
