NTRC_AZOC5
ID NTRC_AZOC5 Reviewed; 480 AA.
AC Q04848; A8IBK8;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 26-FEB-2008, sequence version 2.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=DNA-binding transcriptional regulator NtrC {ECO:0000250|UniProtKB:P0AFB8};
DE AltName: Full=Nitrogen regulation protein NR(I) {ECO:0000250|UniProtKB:P0AFB8};
DE AltName: Full=Nitrogen regulator I {ECO:0000250|UniProtKB:P0AFB8};
DE Short=NRI {ECO:0000250|UniProtKB:P0AFB8};
GN Name=ntrC; OrderedLocusNames=AZC_3086;
OS Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / LMG
OS 6465 / NBRC 14845 / NCIMB 13405 / ORS 571).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Xanthobacteraceae; Azorhizobium.
OX NCBI_TaxID=438753;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43989 / DSM 5975 / JCM 20966 / LMG 6465 / NBRC 14845 / NCIMB
RC 13405 / ORS 571;
RA Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T.,
RA Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B.,
RA Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H.;
RT "Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium
RT caulinodans ORS571.";
RL Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 413-480.
RX PubMed=1661370; DOI=10.1007/bf00293830;
RA Pawlowski K., Klosse U., de Bruijn F.J.;
RT "Characterization of a novel Azorhizobium caulinodans ORS571 two-component
RT regulatory system, NtrY/NtrX, involved in nitrogen fixation and
RT metabolism.";
RL Mol. Gen. Genet. 231:124-138(1991).
CC -!- FUNCTION: Member of the two-component regulatory system NtrB/NtrC,
CC which controls expression of the nitrogen-regulated (ntr) genes in
CC response to nitrogen limitation. Phosphorylated NtrC binds directly to
CC DNA and stimulates the formation of open promoter-sigma54-RNA
CC polymerase complexes. {ECO:0000250|UniProtKB:P0AFB8}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P0AFB8}.
CC -!- PTM: Phosphorylated and dephosphorylated by NtrB.
CC {ECO:0000250|UniProtKB:P0AFB8}.
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DR EMBL; AP009384; BAF89084.1; -; Genomic_DNA.
DR EMBL; X63841; CAA45328.1; -; Genomic_DNA.
DR PIR; S18622; S18622.
DR RefSeq; WP_012171610.1; NC_009937.1.
DR AlphaFoldDB; Q04848; -.
DR SMR; Q04848; -.
DR STRING; 438753.AZC_3086; -.
DR PRIDE; Q04848; -.
DR EnsemblBacteria; BAF89084; BAF89084; AZC_3086.
DR KEGG; azc:AZC_3086; -.
DR eggNOG; COG2204; Bacteria.
DR HOGENOM; CLU_000445_0_1_5; -.
DR OMA; DWTGNIR; -.
DR OrthoDB; 123059at2; -.
DR Proteomes; UP000000270; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0000156; F:phosphorelay response regulator activity; IEA:InterPro.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR GO; GO:0006808; P:regulation of nitrogen utilization; IEA:InterPro.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR002197; HTH_Fis.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR InterPro; IPR002078; Sigma_54_int.
DR InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR InterPro; IPR025943; Sigma_54_int_dom_ATP-bd_2.
DR InterPro; IPR025944; Sigma_54_int_dom_CS.
DR InterPro; IPR010114; Transcript_reg_NtrC.
DR Pfam; PF02954; HTH_8; 1.
DR Pfam; PF00072; Response_reg; 1.
DR Pfam; PF00158; Sigma54_activat; 1.
DR PRINTS; PR01590; HTHFIS.
DR SMART; SM00382; AAA; 1.
DR SMART; SM00448; REC; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR01818; ntrC; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
DR PROSITE; PS00675; SIGMA54_INTERACT_1; 1.
DR PROSITE; PS00676; SIGMA54_INTERACT_2; 1.
DR PROSITE; PS00688; SIGMA54_INTERACT_3; 1.
DR PROSITE; PS50045; SIGMA54_INTERACT_4; 1.
PE 3: Inferred from homology;
KW Activator; ATP-binding; Cytoplasm; DNA-binding; Nitrogen fixation;
KW Nucleotide-binding; Phosphoprotein; Reference proteome; Repressor;
KW Transcription; Transcription regulation; Two-component regulatory system.
FT CHAIN 1..480
FT /note="DNA-binding transcriptional regulator NtrC"
FT /id="PRO_0000081163"
FT DOMAIN 5..119
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DOMAIN 140..368
FT /note="Sigma-54 factor interaction"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT DNA_BIND 450..469
FT /note="H-T-H motif"
FT /evidence="ECO:0000250"
FT BINDING 168..175
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT BINDING 231..240
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT MOD_RES 54
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ SEQUENCE 480 AA; 53253 MW; 29AB7772D1EF1E66 CRC64;
MPTGSILVAD DDAAIRTVLN QALSRAGYEV RSCGNAATLW RWVSQGDGDL VITDVVMPDE
NAFDLLPRIK KARPDLPVIV MSAQNTFMTA IRASERGAYE YLPKPFDLKE LISIVGRALA
EPKKAEKLAL GSDEQENIPL VGRSAAMQEI YRLLARLMQT DLTVMIAGES GTGKELVARA
LHDYGKRRNG PFVAINMAAI PRDLIESELF GHEKGSFTGA TTRNAGRFEQ AEGGTLFLDE
IGDMPMEAQT RLLRVLQQGE YTTVGGRTPI KTDVRIIAAT NKDLRTLIQQ GLFREDLFFR
LNVVPLRLPP LRERSEDVPD LVRHFFLQAE REGLPAKQVD SAALDRLKRY RWPGNVRELE
NLVRRLAALY PQEIITPSII EAELSEPAPS ALSEDGPVDA TLGASVERHL NGYFSGFGED
LPPPGLYHRI LRDVEYPLLS AALAATRGNQ IKAAELLGLN RNTLRKKIRD LDIQVIRTSR
