NTRC_RHOCB
ID NTRC_RHOCB Reviewed; 458 AA.
AC P09432; D5AUA4;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 2.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=DNA-binding transcriptional regulator NtrC {ECO:0000250|UniProtKB:P0AFB8};
DE AltName: Full=Nitrogen regulation protein NR(I) {ECO:0000250|UniProtKB:P0AFB8};
DE AltName: Full=Nitrogen regulator I {ECO:0000250|UniProtKB:P0AFB8};
DE Short=NRI {ECO:0000250|UniProtKB:P0AFB8};
GN Name=ntrC; Synonyms=nifR1; OrderedLocusNames=RCAP_rcc01798;
OS Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC Rhodobacteraceae; Rhodobacter.
OX NCBI_TaxID=272942;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC BAA-309 / NBRC 16581 / SB1003;
RX PubMed=2710108; DOI=10.1007/bf00427050;
RA Jones R., Haselkorn R.;
RT "The DNA sequence of the Rhodobacter capsulatus ntrA, ntrB and ntrC gene
RT analogues required for nitrogen fixation.";
RL Mol. Gen. Genet. 215:507-516(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC BAA-309 / NBRC 16581 / SB1003;
RX PubMed=8355615; DOI=10.1111/j.1365-2958.1993.tb01636.x;
RA Foster-Hartnett D., Cullen P.J., Gabbert K.K., Kranz R.G.;
RT "Sequence, genetic, and lacZ fusion analyses of a nifR3-ntrB-ntrC operon in
RT Rhodobacter capsulatus.";
RL Mol. Microbiol. 8:903-914(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-309 / NBRC 16581 / SB1003;
RX PubMed=20418398; DOI=10.1128/jb.00366-10;
RA Strnad H., Lapidus A., Paces J., Ulbrich P., Vlcek C., Paces V.,
RA Haselkorn R.;
RT "Complete genome sequence of the photosynthetic purple nonsulfur bacterium
RT Rhodobacter capsulatus SB 1003.";
RL J. Bacteriol. 192:3545-3546(2010).
CC -!- FUNCTION: Member of the two-component regulatory system NtrB/NtrC,
CC which controls expression of the nitrogen-regulated (ntr) genes in
CC response to nitrogen limitation. Phosphorylated NtrC binds directly to
CC DNA and stimulates the formation of open promoter-sigma54-RNA
CC polymerase complexes. {ECO:0000250|UniProtKB:P0AFB8}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P0AFB8}.
CC -!- PTM: Phosphorylated and dephosphorylated by NtrB.
CC {ECO:0000250|UniProtKB:P0AFB8}.
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DR EMBL; X12359; CAA30922.1; -; Genomic_DNA.
DR EMBL; X72382; CAA51075.1; -; Genomic_DNA.
DR EMBL; CP001312; ADE85543.1; -; Genomic_DNA.
DR PIR; S03321; S03321.
DR RefSeq; WP_013067522.1; NC_014034.1.
DR AlphaFoldDB; P09432; -.
DR SMR; P09432; -.
DR STRING; 272942.RCAP_rcc01798; -.
DR EnsemblBacteria; ADE85543; ADE85543; RCAP_rcc01798.
DR GeneID; 31490673; -.
DR KEGG; rcp:RCAP_rcc01798; -.
DR eggNOG; COG2204; Bacteria.
DR HOGENOM; CLU_000445_0_1_5; -.
DR OMA; YDYFKKP; -.
DR OrthoDB; 123059at2; -.
DR Proteomes; UP000002361; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR002197; HTH_Fis.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR InterPro; IPR002078; Sigma_54_int.
DR InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR InterPro; IPR025944; Sigma_54_int_dom_CS.
DR Pfam; PF02954; HTH_8; 1.
DR Pfam; PF00072; Response_reg; 1.
DR Pfam; PF14532; Sigma54_activ_2; 1.
DR PRINTS; PR01590; HTHFIS.
DR SMART; SM00382; AAA; 1.
DR SMART; SM00448; REC; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
DR PROSITE; PS00675; SIGMA54_INTERACT_1; 1.
DR PROSITE; PS00688; SIGMA54_INTERACT_3; 1.
DR PROSITE; PS50045; SIGMA54_INTERACT_4; 1.
PE 3: Inferred from homology;
KW Activator; ATP-binding; Cytoplasm; DNA-binding; Nitrogen fixation;
KW Nucleotide-binding; Phosphoprotein; Reference proteome; Repressor;
KW Transcription; Transcription regulation; Two-component regulatory system.
FT CHAIN 1..458
FT /note="DNA-binding transcriptional regulator NtrC"
FT /id="PRO_0000081174"
FT DOMAIN 4..118
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DOMAIN 143..343
FT /note="Sigma-54 factor interaction"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT DNA_BIND 426..445
FT /note="H-T-H motif"
FT /evidence="ECO:0000250"
FT BINDING 171..178
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT MOD_RES 53
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT CONFLICT 66
FT /note="P -> A (in Ref. 1; CAA30922 and 2; CAA51075)"
FT /evidence="ECO:0000305"
FT CONFLICT 121
FT /note="K -> T (in Ref. 1; CAA30922 and 2; CAA51075)"
FT /evidence="ECO:0000305"
FT CONFLICT 216..221
FT /note="VKGGSL -> RQGRLV (in Ref. 1; CAA30922 and 2;
FT CAA51075)"
FT /evidence="ECO:0000305"
FT CONFLICT 248
FT /note="Missing (in Ref. 1; CAA30922 and 2; CAA51075)"
FT /evidence="ECO:0000305"
FT CONFLICT 267
FT /note="R -> RR (in Ref. 1; CAA30922 and 2; CAA51075)"
FT /evidence="ECO:0000305"
FT CONFLICT 313
FT /note="R -> A (in Ref. 1; CAA30922 and 2; CAA51075)"
FT /evidence="ECO:0000305"
FT CONFLICT 366..369
FT /note="DALR -> EPL (in Ref. 1; CAA30922 and 2; CAA51075)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 458 AA; 50044 MW; BC5D30F5E19D5301 CRC64;
MDGTVLVADD DRTIRTVLTQ ALTRAGCKVH ATASLMTLMR WVEEGKGDLV ISDVVMPDGN
GLEALPRIAR ERPGLPVIVI SAQNTIMTAI QAAEADAYDY LPKPFDLPDL MKRAARALEL
KRRAQVVPKV IETSRPEGTD LPLVGRTAAM QALYRLVARV MNADLPVMIM GESGTGKSLI
AKAIHDFSDR RTLPFVVAQA ADLLGADGPS SLLARVKGGS LVFDEVGDYD DETQGRIVRM
LDALPDPAPR IMATTQVDLG ALMEAGRFRQ DLYYRLGGVT LAVPALRERV EDIPLLAEHF
LGRAERDGLG MRRFSAEAMG LVRAYAWPGN VRQLENTVRR LVVTASEEEI TRTEVEFVLG
NQPAVDALRG AGGEGEKLSA SIARHLRRYF DLHGGNLPPP GLYDRILAEM EAPLIEIALD
ATGGNQAKCA DLLGINRNTL RKKITDLDIQ VTRRRKLM
